HAMAP rule MF_01409
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01409 |
| Accession | MF_01409 |
| Dates | 28-FEB-2005 (Created)
31-OCT-2024 (Last updated, Version 20) |
| Name | HMG-CoA_synth_arch |
| Scope(s) |
Archaea |
| Template(s) | A0A384E143; [ Recover all ] |
| Triggered by |
HAMAP; MF_01409 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | HMGCS |
| Protein name | RecName: Full=Hydroxymethylglutaryl-CoA synthase; Short=HMG-CoA synthase; Short=HMGCS; EC=2.3.3.10; |
Comments
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| FUNCTION | Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds that are building blocks of archaeal membrane lipids. |
| CATALYTIC ACTIVITY | Reaction=acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3- methylglutaryl-CoA + CoA + H(+); Xref=Rhea:RHEA:10188, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43074, ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.10; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:10189; |
| PATHWAY | Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3. |
| SUBUNIT | Interacts with acetoacetyl-CoA thiolase that catalyzes the precedent step in the pathway and with a DUF35 protein. The acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site, which allows for efficient coupling of the endergonic thiolase reaction with the exergonic HMGCS reaction. |
| SIMILARITY | Belongs to the thiolase-like superfamily. Archaeal HMG-CoA synthase family. |
Keywords
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Gene Ontology
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| GO:0003985; Molecular function:acetyl-CoA C-acetyltransferase activity |
| GO:0019287; Biological process:isopentenyl diphosphate biosynthetic process, mevalonate pathway |
Cross-references
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| Pfam | PF01154; HMG_CoA_synt_N; 1; |
| Pfam | PF08541; ACP_syn_III_C; 1; |
| NCBIfam | TIGR00748; HMG_CoA_syn_Arc; 1; |
Features
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| From: HMGCS_METAR (Q0W7K1) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 80 | 80 | /note="Proton donor/acceptor" | E | ||||||||
| ACT_SITE | 112 | 112 | /note="Acyl-thioester intermediate" | C | ||||||||
| ACT_SITE | 234 | 234 | /note="Proton donor/acceptor" | H | ||||||||
| BINDING | 29 | 29 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
[DE] | ||||||||
| BINDING | 30 | 30 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
[AV] | ||||||||
| BINDING | 112 | 112 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
C | ||||||||
| BINDING | 153 | 153 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
[TS] | ||||||||
| BINDING | 199 | 199 | /ligand="CoA" /ligand_id="ChEBI:CHEBI:57287" /ligand_note="ligand shared with acetoacetyl-CoA thiolase" |
[RK] | ||||||||
| BINDING | 201 | 201 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
[TS] | ||||||||
| BINDING | 234 | 234 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
H | ||||||||
| BINDING | 239 | 239 | /ligand="CoA" /ligand_id="ChEBI:CHEBI:57287" /ligand_note="ligand shared with acetoacetyl-CoA thiolase" |
[KR] | ||||||||
| BINDING | 243 | 243 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
[RK] | ||||||||
| BINDING | 266 | 266 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
N | ||||||||
| BINDING | 296 | 296 | /ligand="(3S)-hydroxy-3-methylglutaryl-CoA" /ligand_id="ChEBI:CHEBI:43074" |
S | ||||||||
Additional information
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| Size range | 343-365 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |