HAMAP rule MF_01433

General rule information [?]

Accession	MF_01433
Dates	20-DEC-2005 (Created) 19-NOV-2022 (Last updated, Version 19)

Name	Pyrim_hydro_RihB

Scope

Bacteria; Enterobacteriaceae

Template

P33022 (RIHB_ECOLI)

Triggered by

HAMAP; MF_01433 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

RIHB

Protein name

RecName:		Full=Pyrimidine-specific ribonucleoside hydrolase RihB;
		EC 3.2.2.8;
AltName:		Full=Cytidine/uridine-specific hydrolase;

Gene name

rihB

Comments [?]

Function	Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively. Has a clear preference for cytidine over uridine. Strictly specific for ribonucleosides.
Catalytic activity	RHEA:56816: a pyrimidine ribonucleoside + H2O = a pyrimidine nucleobase + D-ribose EC 3.2.2.8

case <FTGroup:1>

Cofactor

Ca(2+)
Note: Binds 1 Ca(2+) ion per monomer.

end case

Subunit	Homotetramer.
Similarity	Belongs to the IUNH family. RihB subfamily.

Keywords [?]

Glycosidase
Hydrolase

case <FTGroup:1>

Calcium
Metal-binding

end case

Gene Ontology [?]

case <FTGroup:1>

GO:0005509; Molecular function: calcium ion binding.

end case

GO:0050263; Molecular function: ribosylpyrimidine nucleosidase activity.
GO:0006206; Biological process: pyrimidine nucleobase metabolic process.

Cross-references [?]

PROSITE	PS01247; IUNH; 1;
Pfam	PF01156; IU_nuc_hydro; 1;

Features [?]

From: RIHB_ECOLI (P33022)

Key From To Description Tag Condition FTGroup

ACT_SITE 11 11 Proton acceptor [DE]

BINDING 11 11 /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108 [DE] 1

BINDING 16 16 /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108 D 1

BINDING 124 124 /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108 V 1

BINDING 240 240 /ligand="Ca(2+)" /ligand_id="ChEBI:CHEBI:29108 D 1

BINDING 227 227 /ligand="substrate Q

BINDING 239 239 /ligand="substrate H

Additional information [?]

Size range	313-313 amino acids
Related rules	None
Fusion	None

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