HAMAP rule MF_01453
General rule information
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Accession | MF_01453 |
Dates | 4-JUN-2009 (Created)
10-NOV-2023 (Last updated, Version 20) |
Name | AddB_type2 |
Scope(s) |
Bacteria Bacillota |
Template(s) | A2RH76 (ADDB_LACLM); Q97QQ0 (ADDB_STRPN); [ Recover all ] |
Triggered by |
HAMAP; MF_01453 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | ADDB |
RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.1.-.-">3.1.-.-</a>;<br /> AltName: Full=ATP-dependent helicase/nuclease subunit RexB; | |
Gene name | Name=rexB; |
Comments
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FUNCTION | The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. This subunit has 5' -> 3' nuclease activity but not helicase activity. |
case <FTGroup:1> | |
COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.; |
end case | |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
SUBUNIT | Heterodimer of AddA and RexB. |
MISCELLANEOUS | Despite having helicase-like domains, this subunit does not have helicase activity. |
SIMILARITY | Belongs to the helicase family. AddB/RexB type 2 subfamily. |
Keywords
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ATP-binding | |
DNA damage | |
DNA repair | |
DNA-binding | |
Exonuclease | |
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a> | |
Hydrolase | |
Nuclease | |
Nucleotide-binding | |
case <FTGroup:1> | |
4Fe-4S | |
Iron | |
Iron-sulfur | |
Metal-binding | |
end case |
Gene Ontology
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GO:0005524; Molecular function:ATP binding | |
GO:0008409; Molecular function:5'-3' exonuclease activity | |
GO:0003690; Molecular function:double-stranded DNA binding | |
case <FTGroup:1> | |
GO:0051536; Molecular function:iron-sulfur cluster binding | |
end case | |
GO:0000724; Biological process:double-strand break repair via homologous recombination |
Cross-references
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Features
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From: ADDB_LACLM (A2RH76) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 766 | 766 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
BINDING | 1056 | 1056 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
BINDING | 1059 | 1059 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
BINDING | 1065 | 1065 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 |
Additional information
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Size range | 1071-1260 amino acids |
Related rules |
MF_01452 |
Fusion | Nter: None Cter: None |
Comments | The iron-sulfur center has been shown to exist in the related AddB protein from B.subtilis; its suggested presence in a few members of this family is based solely on sequence conservation. Two related subfamiles of this protein exist; addB in Bacillus and Staphylococcus (MF_01452), and rexB in Lactobacillus and Streptococcus (this family). In most Lactobacillales there is no 4Fe-4S center; this has been suggested (PubMed:19129187) to occur in organisms which inhabit a low- iron environment. |