Home  |  Contact
Annotation rule MF_01453
Send feedback

General rule information [?]

Accession MF_01453
Dates 4-JUN-2009 (Created)
19-NOV-2019 (Last updated, Version 15)
Name AddB_type2
Scope
Bacteria; Firmicutes
Templates A2RH76 (ADDB_LACLM); Q97QQ0 (ADDB_STRPN): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ADDB
Protein name
RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B;
EC 3.1.-.-;
EC 3.6.4.12;
AltName: Full=ATP-dependent helicase/nuclease RexB;
Gene name
rexB

Comments [?]

Function The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. This subunit has 5' -> 3' nuclease activity.
Catalytic activity RHEA:13065: ATP + H2O = ADP + H(+) + phosphate
EC 3.6.4.12
case <FTGroup:1>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster.
end case
Cofactor Mg(2+)
Subunit Heterodimer of AddA and RexB.
Similarity Belongs to the helicase family. AddB/RexB type 2 subfamily.

Keywords [?]

ATP-binding
DNA damage
DNA repair
Exonuclease
Hydrolase
Nuclease
Nucleotide-binding
case <FTGroup:1>
4Fe-4S
Iron
Iron-sulfur
Metal-binding
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0008409; Molecular function: 5'-3' exonuclease activity.
GO:0003690; Molecular function: double-stranded DNA binding.
case <FTGroup:1>
GO:0051536; Molecular function: iron-sulfur cluster binding.
end case
GO:0000724; Biological process: double-strand break repair via homologous recombination.

Cross-references [?]

TIGRFAMs TIGR02774; RexB_recomb; 1;

Features [?]

From: ADDB_LACLM (A2RH76)
Key     From     To       Description   Tag   Condition   FTGroup
METAL (Optional)     766     766       Iron-sulfur (4Fe-4S)     C   1
METAL (Optional)     1056     1056       Iron-sulfur (4Fe-4S)     C   1
METAL (Optional)     1059     1059       Iron-sulfur (4Fe-4S)     C   1
METAL (Optional)     1065     1065       Iron-sulfur (4Fe-4S)     C   1

Additional information [?]

Size range 1071-1260 amino acids
Related rules MF_01452 (ADDB)
Fusion None
Comments The iron-sulfur center has been shown to exist in the related AddB protein from B.subtilis; its suggested presence in a few members of this family is based solely on sequence conservation. Two related subfamiles of this protein exist; addB in Bacillus and Staphylococcus (MF_01452), and rexB in Lactobacillus and Streptococcus (this family). In most Lactobacillales there is no 4Fe-4S center; this has been suggested (PubMed:19129187) to occur in organisms which inhabit a low- iron environment.


View rule in raw text format (no links)