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HAMAP rule MF_01453

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General rule information [?]

Accession MF_01453
Dates 4-JUN-2009 (Created)
10-NOV-2023 (Last updated, Version 20)
Name AddB_type2
Scope(s) Bacteria
Template(s) A2RH76 (ADDB_LACLM); Q97QQ0 (ADDB_STRPN); [ Recover all ]
Triggered by HAMAP; MF_01453 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ADDB
RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/3.1.-.-">3.1.-.-</a>;<br /> AltName: Full=ATP-dependent helicase/nuclease subunit RexB;
Gene name Name=rexB;

Comments [?]

FUNCTIONThe heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. This subunit has 5' -> 3' nuclease activity but not helicase activity.
case <FTGroup:1>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.;
end case
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNITHeterodimer of AddA and RexB.
MISCELLANEOUSDespite having helicase-like domains, this subunit does not have helicase activity.
SIMILARITYBelongs to the helicase family. AddB/RexB type 2 subfamily.

Keywords [?]

DNA damage
DNA repair
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a>
case <FTGroup:1>
end case

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0008409; Molecular function:5'-3' exonuclease activity
GO:0003690; Molecular function:double-stranded DNA binding
case <FTGroup:1>
GO:0051536; Molecular function:iron-sulfur cluster binding
end case
GO:0000724; Biological process:double-strand break repair via homologous recombination

Cross-references [?]

Pfam PF21445; ADDB_N; 1;
Pfam PF12705; PDDEXK_1; 1;
NCBIfam TIGR02774; RexB_recomb; 0-1;

Features [?]

Key From To Description Tag Condition FTGroup
BINDING 766 766 /ligand="[4Fe-4S] cluster"
C 1
BINDING 1056 1056 /ligand="[4Fe-4S] cluster"
C 1
BINDING 1059 1059 /ligand="[4Fe-4S] cluster"
C 1
BINDING 1065 1065 /ligand="[4Fe-4S] cluster"
C 1

Additional information [?]

Size range 1071-1260 amino acids
Related rules MF_01452
Fusion Nter: None Cter: None
Comments The iron-sulfur center has been shown to exist in the related AddB protein from B.subtilis; its suggested presence in a few members of this family is based solely on sequence conservation. Two related subfamiles of this protein exist; addB in Bacillus and Staphylococcus (MF_01452), and rexB in Lactobacillus and Streptococcus (this family). In most Lactobacillales there is no 4Fe-4S center; this has been suggested (PubMed:19129187) to occur in organisms which inhabit a low- iron environment.

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