HAMAP rule MF_01453
General rule information
[?]
| PURL | https://purl.expasy.org/hamap/rule/MF_01453 |
| Accession | MF_01453 |
| Dates | 04-JUN-2009 (Created)
10-NOV-2023 (Last updated, Version 21) |
| Name | AddB_type2 |
| Scope(s) |
Bacteria Bacillota |
| Template(s) | A2RH76; Q97QQ0; [ Recover all ] |
| Triggered by |
HAMAP; MF_01453 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | ADDB |
| Protein name | RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B; EC=3.1.-.-; AltName: Full=ATP-dependent helicase/nuclease subunit RexB; |
| Gene name | Name=rexB; |
Comments
[?]
| FUNCTION | The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. This subunit has 5' -> 3' nuclease activity but not helicase activity. |
| case <FTGroup:1> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.; |
| end case | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SUBUNIT | Heterodimer of AddA and RexB. |
| MISCELLANEOUS | Despite having helicase-like domains, this subunit does not have helicase activity. |
| SIMILARITY | Belongs to the helicase family. AddB/RexB type 2 subfamily. |
Keywords
[?]
| ATP-binding | |
| DNA damage | |
| DNA repair | |
| DNA-binding | |
| Exonuclease | |
| Helicase | |
| Hydrolase | |
| Nuclease | |
| Nucleotide-binding | |
| case <FTGroup:1> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| end case | |
Gene Ontology
[?]
| GO:0005524; Molecular function:ATP binding | |
| GO:0008409; Molecular function:5'-3' exonuclease activity | |
| GO:0003690; Molecular function:double-stranded DNA binding | |
| case <FTGroup:1> | |
| GO:0051536; Molecular function:iron-sulfur cluster binding | |
| end case | |
| GO:0000724; Biological process:double-strand break repair via homologous recombination | |
Cross-references
[?]
Features
[?]
| From: ADDB_LACLM (A2RH76) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 766 | 766 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 1056 | 1056 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 1059 | 1059 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 1065 | 1065 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
Additional information
[?]
| Size range | 1071-1260 amino acids |
| Related rules |
MF_01452 |
| Fusion | Nter: None Cter: None |
| Comments | The iron-sulfur center has been shown to exist in the related AddB protein from B.subtilis; its suggested presence in a few members of this family is based solely on sequence conservation. Two related subfamiles of this protein exist; addB in Bacillus and Staphylococcus (MF_01452), and rexB in Lactobacillus and Streptococcus (this family). In most Lactobacillales there is no 4Fe-4S center; this has been suggested (PubMed:19129187) to occur in organisms which inhabit a low- iron environment. |