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Annotation rule MF_01470
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General rule information [?]

Accession MF_01470
Dates 26-MAR-2012 (Created)
19-NOV-2022 (Last updated, Version 27)
Name Cas1
Scope
Bacteria
Archaea
Templates Q02ML7 (CAS1_PSEAB); Q46896 (CAS1_ECOLI): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
CAS1
Protein name
RecName: Full=CRISPR-associated endonuclease Cas1;
EC 3.1.-.-;
Gene name
cas1

Comments [?]

Function CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.
Cofactor Mg(2+)
Mn(2+)
Subunit Homodimer, forms a heterotetramer with a Cas2 homodimer.
Similarity Belongs to the CRISPR-associated endonuclease Cas1 family.

Keywords [?]

Antiviral defense
DNA-binding
Endonuclease
Hydrolase
Magnesium
Manganese
Metal-binding
Nuclease

Gene Ontology [?]

GO:0004519; Molecular function: endonuclease activity.
GO:0046872; Molecular function: metal ion binding.
GO:0051607; Biological process: defense response to virus.
GO:0043571; Biological process: maintenance of CRISPR repeat elements.

Cross-references [?]

Pfam PF01867; Cas_Cas1; 1;
TIGRFAMs TIGR00287; Cas1; 1;

Features [?]

From: CAS1_PSEAB (Q02ML7)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     190     190       /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035     E  
BINDING     254     254       /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035     H  
BINDING     268     268       /ligand="Mn(2+)" /ligand_id="ChEBI:CHEBI:29035     [DE]  

Additional information [?]

Size range 250-437 amino acids
Related rules None
Fusion Nter: <Cas4>, <Reverse transcriptase>; Cter: None
Comments Frequently duplicated, there are many subfamiles, this one however is the master family. Sometimes found fused after Cas4 or a reverse transcriptase domain. One of the METRM (Q2FPW6) is missing residues predicted to be involved in metal-binding and is atypical.


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