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HAMAP rule MF_01470

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General rule information [?]

Accession MF_01470
Dates 26-MAR-2012 (Created)
7-DEC-2023 (Last updated, Version 32)
Name Cas1
Scope(s) Bacteria
Template(s) Q02ML7 (CAS1_PSEAB); Q46896 (CAS1_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01470 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CAS1
Protein name RecName: Full=CRISPR-associated endonuclease Cas1;
Gene name Name=cas1;

Comments [?]

FUNCTIONCRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
SUBUNITHomodimer, forms a heterotetramer with a Cas2 homodimer.
SIMILARITYBelongs to the CRISPR-associated endonuclease Cas1 family.

Keywords [?]

Gene Ontology [?]

GO:0004519; Molecular function:endonuclease activity
GO:0046872; Molecular function:metal ion binding
GO:0051607; Biological process:defense response to virus
GO:0043571; Biological process:maintenance of CRISPR repeat elements

Cross-references [?]

Pfam PF01867; Cas_Cas1; 1;
NCBIfam TIGR00287; Cas1; 1;

Features [?]

From: CAS1_PSEAB (Q02ML7)
Key From To Description Tag Condition FTGroup
BINDING 190 190 /ligand="Mn(2+)"
BINDING 254 254 /ligand="Mn(2+)"
BINDING 268 268 /ligand="Mn(2+)"

Additional information [?]

Size range 250-437 amino acids
Related rules None
Fusion Nter: <Cas4>; <Reverse transcriptase> Cter: None
Comments Frequently duplicated, there are many subfamiles, this one however is the master family. Sometimes found fused after Cas4 or a reverse transcriptase domain. One of the METRM (Q2FPW6) is missing residues predicted to be involved in metal-binding and is atypical.

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