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Annotation rule MF_01485
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General rule information [?]

Accession MF_01485
Dates 10-DEC-2013 (Created)
19-NOV-2019 (Last updated, Version 11)
Name RecB
Scope
Bacteria; Actinobacteria
Bacteria; Bacteroidetes
Bacteria; Chlorobi
Bacteria; Deferribacteres
Bacteria; Proteobacteria
Template P08394 (RECB_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RECB
Protein name
RecName: Full=RecBCD enzyme subunit RecB;
EC 3.1.11.5;
AltName: Full=Exonuclease V subunit RecB;
Short=ExoV subunit RecB;
AltName: Full=Helicase/nuclease RecBCD subunit RecB;
Gene name
recB

Comments [?]

Function A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.
Catalytic activity Reaction=Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.; EC=3.1.11.5;
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
Subunit Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA.
Domain The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC.
The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA.
Similarity Belongs to the helicase family. UvrD subfamily.

Keywords [?]

ATP-binding
DNA damage
DNA repair
DNA-binding
Exonuclease
Helicase
Hydrolase
case <FTGroup:1>
Magnesium
Metal-binding
end case
Nuclease
Nucleotide-binding

Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
GO:0003678; Molecular function: DNA helicase activity.
GO:0005524; Molecular function: ATP binding.
GO:0003677; Molecular function: DNA binding.
GO:0000724; Biological process: double-strand break repair via homologous recombination.

Cross-references [?]

PROSITE PS51198; UVRD_HELICASE_ATP_BIND; 1; trigger=PRU00560;
PS51217; UVRD_HELICASE_CTER; 1; trigger=PRU00617;
Pfam PF00580; UvrD-helicase; 1;
PF13361; UvrD_C; 1;
TIGRFAMs TIGR00609; recB; 1;

Features [?]

From: RECB_ECOLI (P08394)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     850       DNA-binding and helicase activity, interacts with RecC        
REGION     900     Cter       Nuclease activity, interacts with RecD and RecA        
ACT_SITE     1080     1080       For nuclease activity     D   1
METAL     956     956       Magnesium; via tele nitrogen     H   1
METAL     1067     1067       Magnesium     D   1
METAL     1080     1080       Magnesium     D   1

Additional information [?]

Size range 987-1496 amino acids
Related rules None
Fusion None


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