HAMAP rule MF_01485
General rule information
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| Accession | MF_01485 |
| Dates | 10-DEC-2013 (Created)
10-NOV-2023 (Last updated, Version 20) |
| Name | RecB |
| Scope(s) |
Bacteria Actinomycetota Bacteroidota Chlamydiota Chlorobiota Deferribacteres Pseudomonadota Spirochaetota |
| Template(s) | P08394 (RECB_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01485 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RECB |
| RecName: Full=RecBCD enzyme subunit RecB;<br /> EC=<a href="https://enzyme.expasy.org/EC/3.1.11.5">3.1.11.5</a>;<br /> EC=<a href="https://enzyme.expasy.org/EC/5.6.2.4">5.6.2.4</a>;<br /> AltName: Full=DNA 3'-5' helicase subunit RecB;<br /> AltName: Full=Exonuclease V subunit RecB;<br /> Short=ExoV subunit RecB;<br /> AltName: Full=Helicase/nuclease RecBCD subunit RecB; | |
| Gene name | Name=recB; |
Comments
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| FUNCTION | A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA. |
| CATALYTIC ACTIVITY | Reaction=Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'- phosphooligonucleotides.; EC=3.1.11.5; |
| CATALYTIC ACTIVITY | Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.; EC=5.6.2.4; |
| CATALYTIC ACTIVITY | Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| SUBUNIT | Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA. |
| DOMAIN | The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC. |
| DOMAIN | The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA. |
| MISCELLANEOUS | In the RecBCD complex, RecB has a slow 3'-5' helicase, an exonuclease activity and loads RecA onto ssDNA, RecD has a fast 5'- 3' helicase activity, while RecC stimulates the ATPase and processivity of the RecB helicase and contributes to recognition of the Chi site. |
| SIMILARITY | Belongs to the helicase family. UvrD subfamily. |
Keywords
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| ATP-binding | |
| DNA damage | |
| DNA repair | |
| DNA-binding | |
| Exonuclease | |
| <a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a> | |
| Hydrolase | |
| Isomerase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
| Nuclease | |
| Nucleotide-binding | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| GO:0043138; Molecular function:3'-5' DNA helicase activity | |
| GO:0005524; Molecular function:ATP binding | |
| GO:0003677; Molecular function:DNA binding | |
| GO:0000724; Biological process:double-strand break repair via homologous recombination | |
Cross-references
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| PROSITE | PS51198; UVRD_HELICASE_ATP_BIND; 1; |
| PROSITE | PS51217; UVRD_HELICASE_CTER; 1; |
| Pfam | PF00580; UvrD-helicase; 1; |
| Pfam | PF13361; UvrD_C; 1; |
| NCBIfam | TIGR00609; recB; 1; |
Features
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| From: RECB_ECOLI (P08394) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | Nter | 850 | /note="DNA-binding and helicase activity, interacts with RecC" | |||||||||
| /note="DNA-binding and helicase activity, interacts with | ||||||||||||
| REGION | 900 | Cter | /note="Nuclease activity, interacts with RecD and RecA" | |||||||||
| ACT_SITE | 1080 | 1080 | /note="For nuclease activity" | D | 1 | |||||||
| BINDING | 956 | 956 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
H | 1 | |||||||
| BINDING | 1067 | 1067 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 1080 | 1080 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
Additional information
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| Size range | 987-1496 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |