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Annotation rule MF_01485 |
Accession | MF_01485 |
Dates | 10-DEC-2013 (Created) 19-NOV-2019 (Last updated, Version 11) |
Name | RecB |
Scope | Bacteria; Actinobacteria
Bacteria; Bacteroidetes
Bacteria; Chlorobi
Bacteria; Deferribacteres
Bacteria; Proteobacteria |
Template | P08394 (RECB_ECOLI) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA. |
Catalytic activity | Reaction=Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.; EC=3.1.11.5; |
Cofactor | Mg(2+) Note: Binds 1 Mg(2+) ion per subunit. |
Subunit | Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA. |
Domain | The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC. |
The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA. | |
Similarity | Belongs to the helicase family. UvrD subfamily. |
PROSITE | PS51198; UVRD_HELICASE_ATP_BIND; 1; trigger=PRU00560; |
PS51217; UVRD_HELICASE_CTER; 1; trigger=PRU00617; | |
Pfam | PF00580; UvrD-helicase; 1; |
PF13361; UvrD_C; 1; | |
TIGRFAMs | TIGR00609; recB; 1; |
From: RECB_ECOLI (P08394) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | Nter | 850 | DNA-binding and helicase activity, interacts with RecC | |||||||||
REGION | 900 | Cter | Nuclease activity, interacts with RecD and RecA | |||||||||
ACT_SITE | 1080 | 1080 | For nuclease activity | D | 1 | |||||||
METAL | 956 | 956 | Magnesium; via tele nitrogen | H | 1 | |||||||
METAL | 1067 | 1067 | Magnesium | D | 1 | |||||||
METAL | 1080 | 1080 | Magnesium | D | 1 |
Size range | 987-1496 amino acids |
Related rules | None |
Fusion | None |