HAMAP rule MF_01487
General rule information
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| Accession | MF_01487 |
| Dates | 17-DEC-2013 (Created)
14-DEC-2023 (Last updated, Version 20) |
| Name | RecD |
| Scope(s) |
Bacteria Actinomycetota Bacteroidota Chlamydiota Chlorobiota Deferribacteres Pseudomonadota Spirochaetota |
| Template(s) | P04993 (RECD_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01487 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RECD |
| RecName: Full=RecBCD enzyme subunit RecD;<br /> EC=<a href="https://enzyme.expasy.org/EC/5.6.2.3">5.6.2.3</a>;<br /> AltName: Full=DNA 5'-3' helicase subunit RecD;<br /> AltName: Full=Exonuclease V subunit RecD;<br /> Short=ExoV subunit RecD;<br /> AltName: Full=Helicase/nuclease RecBCD subunit RecD; | |
| Gene name | Name=recD; |
Comments
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| FUNCTION | A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre- assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD. |
| CATALYTIC ACTIVITY | Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.; EC=5.6.2.3; |
| CATALYTIC ACTIVITY | Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.3; |
| SUBUNIT | Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. |
| MISCELLANEOUS | In the RecBCD complex, RecB has a slow 3'-5' helicase, an exonuclease activity and loads RecA onto ssDNA, RecD has a fast 5'- 3' helicase activity, while RecC stimulates the ATPase and processivity of the RecB helicase and contributes to recognition of the Chi site. |
| SIMILARITY | Belongs to the RecD family. |
Keywords
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| case <FT:1> | |
| ATP-binding | |
| Nucleotide-binding | |
| end case | |
| DNA damage | |
| DNA repair | |
| DNA-binding | |
| Exonuclease | |
| <a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a> | |
| Hydrolase | |
| Isomerase | |
| Nuclease | |
Gene Ontology
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| GO:0043139; Molecular function:5'-3' DNA helicase activity | |
| case <FT:1> | |
| GO:0005524; Molecular function:ATP binding | |
| end case | |
| GO:0003677; Molecular function:DNA binding | |
| GO:0000724; Biological process:double-strand break repair via homologous recombination | |
Cross-references
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Features
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| From: RECD_ECOLI (P04993) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 171 | 178 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-G-P-G-T-G-K-T | ||||||||
Additional information
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| Size range | 554-640 amino acids |
| Related rules |
MF_01488 |
| Fusion | Nter: None Cter: None |
| Comments | There is a related helicase (RecD2, MF_01488) that is not found associated with RecB and RecC, although some organisms have both genes. |