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HAMAP rule MF_01487

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General rule information [?]

Accession MF_01487
Dates 17-DEC-2013 (Created)
14-DEC-2023 (Last updated, Version 20)
Name RecD
Scope(s) Bacteria
Template(s) P04993 (RECD_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01487 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RECD
RecName: Full=RecBCD enzyme subunit RecD;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/"></a>;<br /> AltName: Full=DNA 5'-3' helicase subunit RecD;<br /> AltName: Full=Exonuclease V subunit RecD;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Short=ExoV subunit RecD;<br /> AltName: Full=Helicase/nuclease RecBCD subunit RecD;
Gene name Name=recD;

Comments [?]

FUNCTIONA helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre- assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
CATALYTIC ACTIVITY Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA at the replication fork by translocating in the 5'-3' direction. This creates two antiparallel DNA single strands (ssDNA). The leading ssDNA polymer is the template for DNA polymerase III holoenzyme which synthesizes a continuous strand.; EC=;
CATALYTIC ACTIVITY Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=;
SUBUNITHeterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding.
MISCELLANEOUSIn the RecBCD complex, RecB has a slow 3'-5' helicase, an exonuclease activity and loads RecA onto ssDNA, RecD has a fast 5'- 3' helicase activity, while RecC stimulates the ATPase and processivity of the RecB helicase and contributes to recognition of the Chi site.
SIMILARITYBelongs to the RecD family.

Keywords [?]

case <FT:1>
end case
DNA damage
DNA repair
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a>

Gene Ontology [?]

GO:0043139; Molecular function:5'-3' DNA helicase activity
case <FT:1>
GO:0005524; Molecular function:ATP binding
end case
GO:0003677; Molecular function:DNA binding
GO:0000724; Biological process:double-strand break repair via homologous recombination

Cross-references [?]

Pfam PF21185; RecD_N; 0-1;
Pfam PF13538; UvrD_C_2; 1;
NCBIfam TIGR01447; recD; 1;

Features [?]

From: RECD_ECOLI (P04993)
Key From To Description Tag Condition FTGroup
BINDING 171 178 /ligand="ATP"

Additional information [?]

Size range 554-640 amino acids
Related rules MF_01488
Fusion Nter: None Cter: None
Comments There is a related helicase (RecD2, MF_01488) that is not found associated with RecB and RecC, although some organisms have both genes.

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