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HAMAP rule MF_01487

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General rule information [?]

Accession MF_01487
Dates 17-DEC-2013 (Created)
1-JUN-2023 (Last updated, Version 18)
Name RecD
Bacteria; Actinomycetota
Bacteria; Bacteroidota
Bacteria; Chlorobiota
Bacteria; Deferribacteres
Bacteria; Pseudomonadota
Template P04993 (RECD_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=RecBCD enzyme subunit RecD;
AltName: Full=Exonuclease V subunit RecD;
Short=ExoV subunit RecD;
AltName: Full=Helicase/nuclease RecBCD subunit RecD;
Gene name

Comments [?]

Function A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.
Catalytic activity Reaction=Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.; EC=;
Subunit Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding.
Similarity Belongs to the RecD family.

Keywords [?]

case <FT:1>
end case

Gene Ontology [?]

GO:0003678; Molecular function: DNA helicase activity.
case <FT:1>
GO:0005524; Molecular function: ATP binding.
end case
GO:0003677; Molecular function: DNA binding.
GO:0000724; Biological process: double-strand break repair via homologous recombination.

Cross-references [?]

Pfam PF13538; UvrD_C_2; 1;
NCBIfam TIGR01447; recD; 1;

Features [?]

From: RECD_ECOLI (P04993)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     171     178       /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616     G-G-P-G-T-G-K-T  

Additional information [?]

Size range 575-640 amino acids
Related rules MF_01488 (RECDL supersedes the current rule)
Fusion None
Comments There is a related helicase (RecD-like, MF_01488) that is not found associated with RecB and RecC, although some organisms have both genes.