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HAMAP rule MF_01492

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General rule information [?]

Accession MF_01492
Dates 9-MAY-2014 (Created)
1-JUN-2023 (Last updated, Version 12)
Name RNase_J_arch
Template Q9V076 (RNJ_PYRAB)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Ribonuclease J;
Short=RNase J;
EC 3.1.-.-;
Gene name

Comments [?]

Function An RNase that has 5'-3' exonuclease activity. May be involved in RNA degradation.
Subunit Homodimer.
case <FTGroup:1> or <FTGroup:2>
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily.

Keywords [?]

case <FTGroup:1> or <FTGroup:2>
end case

Gene Ontology [?]

GO:0004534; Molecular function: 5'-3' RNA exonuclease activity.
GO:0006401; Biological process: RNA catabolic process.
case <FTGroup:1> or <FTGroup:2>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS01292; UPF0036; 1;
Pfam PF00753; Lactamase_B; 1;
PF07521; RMMBL; 1;
NCBIfam TIGR00649; MG423; 1;

Features [?]

From: RNJ_PYRAB (Q9V076)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     84     84       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="catalytic     H   1
BINDING     86     86       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="catalytic     H   1
BINDING (Optional)     88     88       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /ligand_note="catalytic     D   2
BINDING (Optional)     89     89       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /ligand_note="catalytic     H   2
BINDING     155     155       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="catalytic     H   1
BINDING     177     177       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" /ligand_note="catalytic     [DE]   1
BINDING (Optional)     177     177       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /ligand_note="catalytic     [DE]   2
BINDING (Optional)     410     410       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" /ligand_note="catalytic     H   2
BINDING     384     388       /ligand="substrate     H-[VTA]-S-[GS]-H  

Additional information [?]

Size range 399-475 amino acids
Related rules MF_01491 (RNJ)
Fusion None
Comments Duplicated in a few Archaea. A closely-related protein exists in bacteria, which is about 100 residues longer at the C-terminus and has exo- and endoribonuclease activity.