HAMAP rule MF_01520
General rule information
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Accession | MF_01520 |
Dates | 13-AUG-2004 (Created)
12-MAR-2024 (Last updated, Version 36) |
Name | IspDF |
Scope(s) |
Bacteria |
Template(s) | Q9PM68 (ISPDF_CAMJE); Q46893 (ISPD_ECOLI); P62617 (ISPF_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_01520 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | ISPDF |
Protein name | RecName: Full=Bifunctional enzyme IspD/IspF; Includes: RecName: Full=2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; EC=2.7.7.60; AltName: Full=4-diphosphocytidyl-2C-methyl-D-erythritol synthase; AltName: Full=MEP cytidylyltransferase; Short=MCT; Includes: RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; Short=MECPP-synthase; Short=MECDP-synthase; Short=MECPS; EC=4.6.1.12; |
Gene name | Name=ispDF; |
Comments
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FUNCTION | Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D- erythritol 4-phosphate (MEP) (IspD), and catalyzes the conversion of 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C- methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP) (IspF). |
CATALYTIC ACTIVITY | Reaction=2-C-methyl-D-erythritol 4-phosphate + CTP + H(+) = 4-CDP-2-C- methyl-D-erythritol + diphosphate; Xref=Rhea:RHEA:13429, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:57823, ChEBI:CHEBI:58262; EC=2.7.7.60; |
CATALYTIC ACTIVITY | Reaction=4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D- erythritol 2,4-cyclic diphosphate + CMP; Xref=Rhea:RHEA:23864, ChEBI:CHEBI:57919, ChEBI:CHEBI:58483, ChEBI:CHEBI:60377; EC=4.6.1.12; |
COFACTOR | Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; |
PATHWAY | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 2/6. |
PATHWAY | Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6. |
SIMILARITY | In the N-terminal section; belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily. |
SIMILARITY | In the C-terminal section; belongs to the IspF family. |
Keywords
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Gene Ontology
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GO:0008685; Molecular function:2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity |
GO:0050518; Molecular function:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity |
GO:0019288; Biological process:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway |
Cross-references
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Pfam | PF01128; IspD; 1; |
Pfam | PF02542; YgbB; 1; |
NCBIfam | TIGR00453; IspD; 1; |
NCBIfam | TIGR00151; IspF; 1; |
PROSITE | PS01295; ISPD; 1; |
PROSITE | PS01350; ISPF; 1; |
Features
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From: ISPDF_CAMJE (Q9PM68) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | Nter | 210 | /note="2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase" | |||||||||
REGION | 211 | Cter | /note="2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase" | |||||||||
BINDING | 217 | 219 | /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919" |
D-x-H | ||||||||
BINDING | 243 | 244 | /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919" |
H-S | ||||||||
BINDING | 265 | 267 | /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919" |
D-[IL]-G | ||||||||
BINDING | 270 | 274 | /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919" |
[FY]-x-D-x-[DNE] | ||||||||
BINDING | 309 | 315 | /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919" |
[AL]-x(2)-P-[KR]-M-[LAG] | ||||||||
BINDING | 341 | 344 | /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919" |
[TS]-[T]-x-[ED] | ||||||||
BINDING | 217 | 217 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
D | ||||||||
BINDING | 219 | 219 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
H | ||||||||
BINDING | 251 | 251 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" |
H | ||||||||
BINDING | 348 | 348 | /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919" |
[FY] | ||||||||
BINDING | 351 | 351 | /ligand="4-CDP-2-C-methyl-D-erythritol 2-phosphate" /ligand_id="ChEBI:CHEBI:57919" |
[RK] | ||||||||
SITE | 16 | 16 | /note="Transition state stabilizer" | R | ||||||||
SITE | 23 | 23 | /note="Transition state stabilizer" | K | ||||||||
SITE | 139 | 139 | /note="Positions MEP for the nucleophilic attack" | [RH] | ||||||||
SITE | 191 | 191 | /note="Positions MEP for the nucleophilic attack" | K | ||||||||
SITE | 243 | 243 | /note="Transition state stabilizer" | [HDN] | ||||||||
SITE | 342 | 342 | /note="Transition state stabilizer" | [TS] |
Additional information
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Size range | 359-422 amino acids |
Related rules |
MF_00107 MF_00108 |
Fusion | Nter: None Cter: None |
Comments | See MF_00108 and MF_00107 for monofunctional ispD and ispF |