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Annotation rule MF_01541
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General rule information [?]

Accession MF_01541
Dates 22-AUG-2005 (Created)
19-NOV-2019 (Last updated, Version 17)
Name CysJ
Scope
Bacteria; Proteobacteria
Templates P38038 (CYSJ_ECOLI); P38039 (CYSJ_SALTY): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
CYSJ
Protein name
RecName: Full=Sulfite reductase [NADPH] flavoprotein alpha-component;
Short=SiR-FP;
EC 1.8.1.2;
Gene name
cysJ

Comments [?]

Function Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component.
Catalytic activity RHEA:13801: 3 H2O + hydrogen sulfide + 3 NADP(+) = 4 H(+) + 3 NADPH + sulfite
EC 1.8.1.2
Cofactor FAD
Note: Binds 1 FAD per subunit.
FMN
Note: Binds 1 FMN per subunit.
Pathway Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.
Subunit Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein.
Similarity Belongs to the NADPH-dependent sulphite reductase flavoprotein subunit CysJ family.
In the N-terminal section; belongs to the flavodoxin family.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Keywords [?]


Gene Ontology [?]

GO:0004783; Molecular function: sulfite reductase (NADPH) activity.
GO:0000103; Biological process: sulfate assimilation.
GO:0070814; Biological process: hydrogen sulfide biosynthetic process.

Cross-references [?]

PROSITE PS50902; FLAVODOXIN_LIKE; 1;
PS51384; FAD_FR; 1; trigger=PRU00716;
Pfam PF00667; FAD_binding_1; 1;
PF00258; Flavodoxin_1; 1;
PF00175; NAD_binding_1; 1;
PRINTS PR00369; FLAVODOXIN; 1;
PR00371; FPNCR; 1;
TIGRFAMs TIGR01931; CysJ; 1;

Features [?]

From: CYSJ_ECOLI (P38038)
Key     From     To       Description   Tag   Condition   FTGroup
DOMAIN     64     202       Flavodoxin-like        
NP_BIND     70     75       FMN     S-Q-T-G-N-[AG]  
NP_BIND     117     120       FMN     S-T-[QHN]-G  
NP_BIND     153     162       FMN     L-G-D-x-[ST]-Y-x(2)-F-[CA]  
NP_BIND     386     389       FAD     R-[LF]-Y-S  
NP_BIND     404     406       FAD     [ST]-V-[GAS]  
NP_BIND     419     422       FAD     G-[GV]-[AS]-S  
NP_BIND     519     520       NADP     S-[RQ]  
NP_BIND     525     529       NADP     K-[IV]-Y-V-Q  
BINDING     322     322       FAD     T  
BINDING     356     356       FAD; via carbonyl oxygen        
BINDING (Optional)     410     410       FAD     Y  
BINDING     561     561       NADP     D  
BINDING (Optional)     599     599       FAD     Y  

Additional information [?]

Size range 595-623 amino acids
Related rules None
Fusion None