HAMAP rule MF_01541
General rule information
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| Accession | MF_01541 |
| Dates | 22-AUG-2005 (Created)
1-JUN-2023 (Last updated, Version 21) |
| Name | CysJ |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P38038 (CYSJ_ECOLI); P38039 (CYSJ_SALTY); [ Recover all ] |
| Triggered by |
HAMAP; MF_01541 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CYSJ |
| Protein name | RecName: Full=Sulfite reductase [NADPH] flavoprotein alpha-component; Short=SiR-FP; EC=1.8.1.2; |
| Gene name | Name=cysJ; |
Comments
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| FUNCTION | Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component. |
| CATALYTIC ACTIVITY | Reaction=3 H2O + hydrogen sulfide + 3 NADP(+) = 4 H(+) + 3 NADPH + sulfite; Xref=Rhea:RHEA:13801, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17359, ChEBI:CHEBI:29919, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.8.1.2; |
| COFACTOR | Name=FAD; Xref=ChEBI:CHEBI:57692; Note=Binds 1 FAD per subunit.; |
| COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per subunit.; |
| PATHWAY | Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. |
| SUBUNIT | Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein. |
| SIMILARITY | Belongs to the NADPH-dependent sulphite reductase flavoprotein subunit CysJ family. |
| SIMILARITY | In the N-terminal section; belongs to the flavodoxin family. |
| SIMILARITY | In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. |
Keywords
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| Amino-acid biosynthesis |
| Cysteine biosynthesis |
| Electron transport |
| FAD |
| Flavoprotein |
| FMN |
| NADP |
| Oxidoreductase |
| Transport |
Gene Ontology
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| GO:0004783; Molecular function:sulfite reductase (NADPH) activity |
| GO:0000103; Biological process:sulfate assimilation |
| GO:0070814; Biological process:hydrogen sulfide biosynthetic process |
Cross-references
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| PROSITE | PS50902; FLAVODOXIN_LIKE; 1; |
| PROSITE | PS51384; FAD_FR; 1; |
| Pfam | PF00667; FAD_binding_1; 1; |
| Pfam | PF00258; Flavodoxin_1; 1; |
| Pfam | PF00175; NAD_binding_1; 1; |
| PRINTS | PR00369; FLAVODOXIN; 1; |
| PRINTS | PR00371; FPNCR; 1; |
| NCBIfam | TIGR01931; CysJ; 1; |
Features
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| From: CYSJ_ECOLI (P38038) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | 64 | 202 | /note="Flavodoxin-like" | |||||||||
| BINDING | 70 | 75 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
S-Q-T-G-N-[AG] | ||||||||
| BINDING | 117 | 120 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
S-T-[QHN]-G | ||||||||
| BINDING | 153 | 162 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
L-G-D-x-[ST]-Y-x(2)-F-[CA] | ||||||||
| BINDING | 386 | 389 | /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692" |
R-[LF]-Y-S | ||||||||
| BINDING | 404 | 406 | /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692" |
[ST]-V-[GAS] | ||||||||
| BINDING | 419 | 422 | /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692" |
G-[GV]-[AS]-S | ||||||||
| BINDING | 519 | 520 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
S-[RQ] | ||||||||
| BINDING | 525 | 529 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
K-[IV]-Y-V-Q | ||||||||
| BINDING | 322 | 322 | /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692" |
T | ||||||||
| BINDING | 356 | 356 | /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692" |
|||||||||
| BINDING | 410 | 410 | /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692" |
Y | ||||||||
| BINDING | 561 | 561 | /ligand="NADP(+)" /ligand_id="ChEBI:CHEBI:58349" |
D | ||||||||
| BINDING | 599 | 599 | /ligand="FAD" /ligand_id="ChEBI:CHEBI:57692" |
Y | ||||||||
Additional information
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| Size range | 595-623 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |