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| Annotation rule MF_01541 |
General rule information
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| Accession | MF_01541 |
| Dates | 22-AUG-2005 (Created) 19-NOV-2019 (Last updated, Version 17) |
| Name | CysJ |
| Scope | Bacteria; Proteobacteria |
| Templates | P38038 (CYSJ_ECOLI); P38039 (CYSJ_SALTY): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component. |
| Catalytic activity | RHEA:13801: 3 H2O + hydrogen sulfide + 3 NADP(+) = 4 H(+) + 3 NADPH + sulfite
EC 1.8.1.2 |
| Cofactor | FAD Note: Binds 1 FAD per subunit. |
| FMN Note: Binds 1 FMN per subunit. |
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| Pathway | Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. |
| Subunit | Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein. |
| Similarity | Belongs to the NADPH-dependent sulphite reductase flavoprotein subunit CysJ family. |
| In the N-terminal section; belongs to the flavodoxin family. | |
| In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. |
Keywords
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Amino-acid biosynthesis
Cysteine biosynthesis
Electron transport
FAD
Flavoprotein
FMN
NADP
Oxidoreductase
Transport
Cysteine biosynthesis
Electron transport
FAD
Flavoprotein
FMN
NADP
Oxidoreductase
Transport
Gene Ontology
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GO:0004783; Molecular function: sulfite reductase (NADPH) activity.
GO:0000103; Biological process: sulfate assimilation.
GO:0070814; Biological process: hydrogen sulfide biosynthetic process.
GO:0000103; Biological process: sulfate assimilation.
GO:0070814; Biological process: hydrogen sulfide biosynthetic process.
Cross-references
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| PROSITE | PS50902; FLAVODOXIN_LIKE; 1; |
| PS51384; FAD_FR; 1; trigger=PRU00716; | |
| Pfam | PF00667; FAD_binding_1; 1; |
| PF00258; Flavodoxin_1; 1; | |
| PF00175; NAD_binding_1; 1; | |
| PRINTS | PR00369; FLAVODOXIN; 1; |
| PR00371; FPNCR; 1; | |
| TIGRFAMs | TIGR01931; CysJ; 1; |
Features
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| From: CYSJ_ECOLI (P38038) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | 64 | 202 | Flavodoxin-like | |||||||||
| NP_BIND | 70 | 75 | FMN | S-Q-T-G-N-[AG] | ||||||||
| NP_BIND | 117 | 120 | FMN | S-T-[QHN]-G | ||||||||
| NP_BIND | 153 | 162 | FMN | L-G-D-x-[ST]-Y-x(2)-F-[CA] | ||||||||
| NP_BIND | 386 | 389 | FAD | R-[LF]-Y-S | ||||||||
| NP_BIND | 404 | 406 | FAD | [ST]-V-[GAS] | ||||||||
| NP_BIND | 419 | 422 | FAD | G-[GV]-[AS]-S | ||||||||
| NP_BIND | 519 | 520 | NADP | S-[RQ] | ||||||||
| NP_BIND | 525 | 529 | NADP | K-[IV]-Y-V-Q | ||||||||
| BINDING | 322 | 322 | FAD | T | ||||||||
| BINDING | 356 | 356 | FAD; via carbonyl oxygen | |||||||||
| BINDING (Optional) | 410 | 410 | FAD | Y | ||||||||
| BINDING | 561 | 561 | NADP | D | ||||||||
| BINDING (Optional) | 599 | 599 | FAD | Y | ||||||||
Additional information
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| Size range | 595-623 amino acids |
| Related rules | None |
| Fusion | None |