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HAMAP rule MF_01569

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General rule information [?]

Accession MF_01569
Dates 18-AUG-2006 (Created)
1-JUN-2023 (Last updated, Version 16)
Name Pro_tRNA_synth_type1
Scope(s) Bacteria
Template(s) P16659 (SYP_ECOLI); O66690 (SYP_AQUAE); [ Recover all ]
Triggered by HAMAP; MF_01569 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SYP
Protein name RecName: Full=Proline--tRNA ligase;
AltName: Full=Prolyl-tRNA synthetase;
Gene name Name=proS;

Comments [?]

FUNCTIONCatalyzes the attachment of proline to tRNA(Pro) in a two- step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala- tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.
CATALYTIC ACTIVITY Reaction=ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl- tRNA(Pro); Xref=Rhea:RHEA:14305, Rhea:RHEA-COMP:9700, Rhea:RHEA- COMP:9702, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:60039, ChEBI:CHEBI:78442, ChEBI:CHEBI:78532, ChEBI:CHEBI:456215; EC=;
DOMAINConsists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.
SIMILARITYBelongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004827; Molecular function:proline-tRNA ligase activity
GO:0006433; Biological process:prolyl-tRNA aminoacylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF03129; HGTP_anticodon; 1;
Pfam PF00587; tRNA-synt_2b; 1;
Pfam PF04073; YbaK; 1;
NCBIfam TIGR00409; ProS_fam_II; 1;

Features [?]

Additional information [?]

Size range 557-620 amino acids
Related rules MF_01570
Fusion Nter: None Cter: None
Comments Some species have a second copy of proS in another subfamily (MF_01571).

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