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Annotation rule MF_01569
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General rule information [?]

Accession MF_01569
Dates 18-AUG-2006 (Created)
19-NOV-2019 (Last updated, Version 15)
Name Pro_tRNA_synth_type1
Scope
Bacteria
Templates P16659 (SYP_ECOLI); O66690 (SYP_AQUAE): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
SYP
Protein name
RecName: Full=Proline--tRNA ligase;
EC 6.1.1.15;
AltName: Full=Prolyl-tRNA synthetase;
Short=ProRS;
Gene name
proS

Comments [?]

Function Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.
Catalytic activity RHEA:14305: ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
EC 6.1.1.15
Subunit Homodimer.
Subcellular location Cytoplasm.
Domain Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.
Similarity Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Keywords [?]

Cytoplasm
Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004827; Molecular function: proline-tRNA ligase activity.
GO:0006433; Biological process: prolyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS50862; AA_TRNA_LIGASE_II; 1;
Pfam PF03129; HGTP_anticodon; 1;
PF00587; tRNA-synt_2b; 1;
PF04073; YbaK; 1;
PRINTS PR01046; TRNASYNTHPRO; 1;
TIGRFAMs TIGR00409; ProS_fam_II; 1;

Additional information [?]

Size range 557-620 amino acids
Related rules MF_01570 (SYP); MF_01571 (SYP)
Fusion None
Comments Some species have a second copy of proS in another subfamily (MF_01571).


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