HAMAP rule MF_01578
General rule information
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| Accession | MF_01578 |
| Dates | 23-FEB-2007 (Created)
19-NOV-2022 (Last updated, Version 19) |
| Name | Shikimate_DH_YdiB |
| Scope(s) |
Bacteria Enterobacterales |
| Template(s) | P0A6D5 (YDIB_ECOLI); Q8ZPR4 (YDIB_SALTY); [ Recover all ] |
| Triggered by |
HAMAP; MF_01578 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | YDIB |
| Protein name | RecName: Full=Quinate/shikimate dehydrogenase; EC=1.1.1.282; AltName: Full=NAD-dependent shikimate 5-dehydrogenase; |
| Gene name | Name=ydiB; |
Comments
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| FUNCTION | The actual biological function of YdiB remains unclear, nor is it known whether 3-dehydroshikimate or quinate represents the natural substrate. Catalyzes the reversible NAD-dependent reduction of both 3-dehydroshikimate (DHSA) and 3-dehydroquinate to yield shikimate (SA) and quinate, respectively. It can use both NAD or NADP for catalysis, however it has higher catalytic efficiency with NAD. |
| CATALYTIC ACTIVITY | Reaction=L-quinate + NAD(+) = 3-dehydroquinate + H(+) + NADH; Xref=Rhea:RHEA:22364, ChEBI:CHEBI:15378, ChEBI:CHEBI:29751, ChEBI:CHEBI:32364, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.282; |
| CATALYTIC ACTIVITY | Reaction=L-quinate + NADP(+) = 3-dehydroquinate + H(+) + NADPH; Xref=Rhea:RHEA:18425, ChEBI:CHEBI:15378, ChEBI:CHEBI:29751, ChEBI:CHEBI:32364, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.282; |
| CATALYTIC ACTIVITY | Reaction=NADP(+) + shikimate = 3-dehydroshikimate + H(+) + NADPH; Xref=Rhea:RHEA:17737, ChEBI:CHEBI:15378, ChEBI:CHEBI:16630, ChEBI:CHEBI:36208, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.282; |
| CATALYTIC ACTIVITY | Reaction=NAD(+) + shikimate = 3-dehydroshikimate + H(+) + NADH; Xref=Rhea:RHEA:17741, ChEBI:CHEBI:15378, ChEBI:CHEBI:16630, ChEBI:CHEBI:36208, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.1.1.282; |
| PATHWAY | Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the shikimate dehydrogenase family. |
Keywords
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Gene Ontology
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| GO:0030266; Molecular function:quinate 3-dehydrogenase (NAD+) activity |
| GO:0004764; Molecular function:shikimate 3-dehydrogenase (NADP+) activity |
| GO:0009073; Biological process:aromatic amino acid family biosynthetic process |
Cross-references
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Features
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| From: YDIB_ECOLI (P0A6D5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 132 | 135 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
A-G-G-A | ||||||||
| BINDING | 155 | 158 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
N-R-[RK]-D | ||||||||
| BINDING | 232 | 235 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
C-x(2)-N | ||||||||
| BINDING | 71 | 71 | /ligand="substrate" | K | ||||||||
| BINDING | 107 | 107 | /ligand="substrate" | D | ||||||||
| BINDING | 205 | 205 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
K | ||||||||
| BINDING | 255 | 255 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
G | ||||||||
Additional information
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| Size range | 288-297 amino acids |
| Related rules |
MF_00222 |
| Fusion | Nter: None Cter: None |
| Comments | Divergent N-terminus in SHIDS and SHIF8; sequences not included in alignment and not taken into account in size range |