HAMAP rule MF_01588
General rule information
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| Accession | MF_01588 |
| Dates | 16-NOV-2007 (Created) 1-JUN-2023 (Last updated, Version 21) |
| Name | DNA_ligase_A |
| Scope | Bacteria
Archaea |
| Templates | O87703 (DNLJ_GEOSE); P15042 (DNLJ_ECOLI); Q9AIU7 (DNLJ_STAAU); P9WNV1 (DNLJ_MYCTU); Q837V6 (DNLJ_ENTFA); Q9ZHI0 (DNLJ_THEFI): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
| Catalytic activity | Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.; EC=6.5.1.2; |
| Cofactor | Mg(2+) Mn(2+) |
| Similarity | Belongs to the NAD-dependent DNA ligase family. LigA subfamily. |
Keywords
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Gene Ontology
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GO:0003911; Molecular function: DNA ligase (NAD+) activity.
GO:0006259; Biological process: DNA metabolic process.
GO:0006259; Biological process: DNA metabolic process.
Cross-references
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| PROSITE | PS50172; BRCT; 1; trigger=PRU00033; |
| PS01055; DNA_LIGASE_N1; 1; | |
| PS01056; DNA_LIGASE_N2; 1; | |
| Pfam | PF00533; BRCT; 1; |
| PF01653; DNA_ligase_aden; 1; | |
| PF03120; DNA_ligase_OB; 1; | |
| PF03119; DNA_ligase_ZBD; 1; | |
| PIRSF | PIRSF001604; LigA; 1; |
| NCBIfam | TIGR00575; Dnlj; 1; |
Features
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| From: DNLJ_ENTFA (Q837V6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 39 | 43 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | D-x-x-[YF]-D | ||||||||
| BINDING | 88 | 89 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | S-x | ||||||||
| ACT_SITE | 120 | 120 | N6-AMP-lysine intermediate | K | ||||||||
| BINDING | 409 | 409 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
| BINDING | 412 | 412 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
| BINDING | 427 | 427 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
| BINDING | 432 | 432 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105 | C | ||||||||
| BINDING (Optional) | 118 | 118 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | [ED] | ||||||||
| BINDING | 141 | 141 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | R | ||||||||
| BINDING | 175 | 175 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | E | ||||||||
| BINDING (Optional) | 291 | 291 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | K | ||||||||
| BINDING | 315 | 315 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540 | K | ||||||||
Additional information
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| Size range | 587-921 amino acids |
| Related rules | MF_01577 (MDTD supersedes the current rule) |
| Fusion | None |