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Annotation rule MF_01588
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General rule information [?]

Accession MF_01588
Dates 16-NOV-2007 (Created)
19-NOV-2019 (Last updated, Version 17)
Name DNA_ligase_A
Templates O87703 (DNLJ_GEOSE); P15042 (DNLJ_ECOLI); Q9AIU7 (DNLJ_STAAU); P9WNV1 (DNLJ_MYCTU); Q837V6 (DNLJ_ENTFA); Q9ZHI0 (DNLJ_THEFI): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=DNA ligase;
AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)];
Gene name

Comments [?]

Function DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity Reaction=NAD(+) + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide.; EC=;
Cofactor Mg(2+)
Similarity Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003911; Molecular function: DNA ligase (NAD+) activity.
GO:0006259; Biological process: DNA metabolic process.

Cross-references [?]

PROSITE PS50172; BRCT; 1; trigger=PRU00033;
PS01055; DNA_LIGASE_N1; 1;
PS01056; DNA_LIGASE_N2; 1;
Pfam PF00533; BRCT; 1;
PF01653; DNA_ligase_aden; 1;
PF03120; DNA_ligase_OB; 1;
PF03119; DNA_ligase_ZBD; 1;
PIRSF PIRSF001604; LigA; 1;
TIGRFAMs TIGR00575; Dnlj; 1;

Features [?]

From: DNLJ_ENTFA (Q837V6)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     39     43       NAD     D-x-x-[YF]-D  
NP_BIND     88     89       NAD     S-x  
ACT_SITE     120     120       N6-AMP-lysine intermediate     K  
METAL     409     409       Zinc     C  
METAL     412     412       Zinc     C  
METAL     427     427       Zinc     C  
METAL     432     432       Zinc     C  
BINDING (Optional)     118     118       NAD     [ED]  
BINDING     141     141       NAD     R  
BINDING     175     175       NAD     E  
BINDING (Optional)     291     291       NAD     K  
BINDING     315     315       NAD     K  

Additional information [?]

Size range 587-921 amino acids
Related rules MF_01577 (MDTD supersedes the current rule)
Fusion None