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HAMAP rule MF_01588

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General rule information [?]

Accession MF_01588
Dates 16-NOV-2007 (Created)
1-JUN-2023 (Last updated, Version 21)
Name DNA_ligase_A
Templates O87703 (DNLJ_GEOSE); P15042 (DNLJ_ECOLI); Q9AIU7 (DNLJ_STAAU); P9WNV1 (DNLJ_MYCTU); Q837V6 (DNLJ_ENTFA); Q9ZHI0 (DNLJ_THEFI): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=DNA ligase;
AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)];
Gene name

Comments [?]

Function DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Catalytic activity Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.; EC=;
Cofactor Mg(2+)
Similarity Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003911; Molecular function: DNA ligase (NAD+) activity.
GO:0006259; Biological process: DNA metabolic process.

Cross-references [?]

PROSITE PS50172; BRCT; 1; trigger=PRU00033;
PS01055; DNA_LIGASE_N1; 1;
PS01056; DNA_LIGASE_N2; 1;
Pfam PF00533; BRCT; 1;
PF01653; DNA_ligase_aden; 1;
PF03120; DNA_ligase_OB; 1;
PF03119; DNA_ligase_ZBD; 1;
PIRSF PIRSF001604; LigA; 1;
NCBIfam TIGR00575; Dnlj; 1;

Features [?]

From: DNLJ_ENTFA (Q837V6)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     39     43       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     D-x-x-[YF]-D  
BINDING     88     89       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     S-x  
ACT_SITE     120     120       N6-AMP-lysine intermediate     K  
BINDING     409     409       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C  
BINDING     412     412       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C  
BINDING     427     427       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C  
BINDING     432     432       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105     C  
BINDING (Optional)     118     118       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     [ED]  
BINDING     141     141       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     R  
BINDING     175     175       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     E  
BINDING (Optional)     291     291       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     K  
BINDING     315     315       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540     K  

Additional information [?]

Size range 587-921 amino acids
Related rules MF_01577 (MDTD supersedes the current rule)
Fusion None