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HAMAP rule MF_01595

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General rule information [?]

Accession MF_01595
Dates 19-MAR-2008 (Created)
19-NOV-2022 (Last updated, Version 19)
Name PNPase
Scope
Bacteria
Templates P05055 (PNP_ECOLI); Q8ZLT3 (PNP_SALTY); P50849 (PNP_BACSU); Q53597 (PNP_STRAT): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PNP
Protein name
RecName: Full=Polyribonucleotide nucleotidyltransferase;
EC 2.7.7.8;
AltName: Full=Polynucleotide phosphorylase;
Short=PNPase;
Gene name
pnp

Comments [?]

Function Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.
Catalytic activity RHEA:22096: phosphate + RNA(n+1) = a ribonucleoside 5'-diphosphate + RNA(n)
EC 2.7.7.8
Cofactor Mg(2+)
case <OC:Gammaproteobacteria>
Subunit Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the polyribonucleotide nucleotidyltransferase family.

Keywords [?]

Cytoplasm
Magnesium
Metal-binding
Nucleotidyltransferase
RNA-binding
Transferase

Gene Ontology [?]

GO:0004654; Molecular function: polyribonucleotide nucleotidyltransferase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0003723; Molecular function: RNA binding.
GO:0006402; Biological process: mRNA catabolic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS50084; KH_TYPE_1; 1; trigger=PRU00117;
PS50126; S1; 1; trigger=PRU00180;
Pfam PF00013; KH_1; 1;
PF03726; PNPase; 1;
PF01138; RNase_PH; 2;
PF03725; RNase_PH_C; 2;
PF00575; S1; 1;
PIRSF PIRSF005499; PNPase; 1;
TIGRFAMs TIGR03591; Polynuc_phos; 1;
TIGR02696; PppGpp_PNP; 1;

Features [?]

From: PNP_ECOLI (P05055)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     486     486       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D  
BINDING     492     492       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420     D  

Additional information [?]

Size range 686-914 amino acids
Related rules None
Fusion None


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