HAMAP rule MF_01602
General rule information
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| Accession | MF_01602 |
| Dates | 17-MAR-2004 (Created)
3-SEP-2024 (Last updated, Version 26) |
| Name | LplA |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P32099 (LPLA_ECOLI); Q9HKT1 (LPLAN_THEAC); [ Recover all ] |
| Triggered by |
HAMAP; MF_01602 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | LPLA |
| Protein name | RecName: Full=Lipoate-protein ligase A; EC=6.3.1.20; AltName: Full=Lipoate--protein ligase; |
| Gene name | Name=lplA; |
Comments
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| FUNCTION | Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. |
| CATALYTIC ACTIVITY | Reaction=L-lysyl-[lipoyl-carrier protein] + (R)-lipoate + ATP = N(6)- [(R)-lipoyl]-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+); Xref=Rhea:RHEA:49288, Rhea:RHEA-COMP:10500, Rhea:RHEA- COMP:10502, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:83088, ChEBI:CHEBI:83099, ChEBI:CHEBI:456215; EC=6.3.1.20; |
| PATHWAY | Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2. |
| PATHWAY | Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2. |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| MISCELLANEOUS | In the transfer reaction, the free carboxyl group of lipoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes. |
| SIMILARITY | Belongs to the LplA family. |
Keywords
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Gene Ontology
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| GO:0016979; Molecular function:lipoate-protein ligase activity |
| GO:0009249; Biological process:protein lipoylation |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| PROSITE | PS51733; BPL_LPL_CATALYTIC; 1; |
| Pfam | PF03099; BPL_LplA_LipB; 1; |
| NCBIfam | TIGR00545; Lipoyltrans; 1; |
Features
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| From: LPLA_ECOLI (P32099) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 76 | 79 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G-A-V-[FY] | ||||||||
| BINDING | 71 | 71 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R | ||||||||
| BINDING | 134 | 134 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| BINDING | 134 | 134 | /ligand="(R)-lipoate" /ligand_id="ChEBI:CHEBI:83088" |
K | ||||||||
Additional information
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| Size range | 337-339 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |