HAMAP rule MF_01602
General rule information
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| Accession | MF_01602 |
| Dates | 17-MAR-2004 (Created) 26-JAN-2023 (Last updated, Version 23) |
| Name | LplA |
| Scope | Bacteria; Pseudomonadota |
| Templates | P32099 (LPLA_ECOLI); Q9HKT1 (LPLAN_THEAC): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Function | Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. |
| Catalytic activity | RHEA:49288: (R)-lipoate + ATP + L-lysyl-[lipoyl-carrier protein] = (R)-N(6)-lipoyl-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+)
EC 6.3.1.20 |
| Pathway | Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2. |
| Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2. | |
| Subunit | Monomer. |
| Subcellular location | Cytoplasm. |
| Miscellaneous | In the transfer reaction, the free carboxyl group of lipoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes. |
| Similarity | Belongs to the LplA family. |
Keywords
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Gene Ontology
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GO:0016979; Molecular function: lipoate-protein ligase activity.
GO:0009249; Biological process: protein lipoylation.
GO:0005737; Cellular component: cytoplasm.
GO:0009249; Biological process: protein lipoylation.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| PROSITE | PS51733; BPL_LPL_CATALYTIC; 1; trigger=PRU01067; |
| Pfam | PF03099; BPL_LplA_LipB; 1; |
| TIGRFAMs | TIGR00545; Lipoyltrans; 1; |
Features
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| From: LPLA_ECOLI (P32099) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 76 | 79 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G-A-V-[FY] | ||||||||
| BINDING | 71 | 71 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | R | ||||||||
| BINDING | 134 | 134 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | K | ||||||||
| BINDING | 134 | 134 | /ligand="(R)-lipoate" /ligand_id="ChEBI:CHEBI:83088 | K | ||||||||
Additional information
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| Size range | 337-339 amino acids |
| Related rules | None |
| Fusion | None |