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Annotation rule MF_01602
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General rule information [?]

Accession MF_01602
Dates 17-MAR-2004 (Created)
1-APR-2020 (Last updated, Version 20)
Name LplA
Scope
Bacteria; Proteobacteria
Templates P32099 (LPLA_ECOLI); Q9HKT1 (LPLAN_THEAC): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
LPLA
Protein name
RecName: Full=Lipoate-protein ligase A;
EC 6.3.1.20;
AltName: Full=Lipoate--protein ligase;
Gene name
lplA

Comments [?]

Function Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes.
Catalytic activity RHEA:49288: (R)-lipoate + ATP + L-lysyl-[lipoyl-carrier protein] = (R)-N(6)-lipoyl-L-lysyl-[lipoyl-carrier protein] + AMP + diphosphate + H(+)
EC 6.3.1.20
Pathway Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 1/2.
Protein modification; protein lipoylation via exogenous pathway; protein N(6)-(lipoyl)lysine from lipoate: step 2/2.
Subunit Monomer.
Subcellular location Cytoplasm.
Miscellaneous In the transfer reaction, the free carboxyl group of lipoic acid is attached via an amide linkage to the epsilon-amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.
Similarity Belongs to the LplA family.

Keywords [?]

ATP-binding
Cytoplasm
Nucleotide-binding
Ligase

Gene Ontology [?]

GO:0016979; Molecular function: lipoate-protein ligase activity.
GO:0009249; Biological process: protein lipoylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS51733; BPL_LPL_CATALYTIC; 1; trigger=PRU01067;
Pfam PF03099; BPL_LplA_LipB; 1;
TIGRFAMs TIGR00545; Lipoyltrans; 1;

Features [?]

From: LPLA_ECOLI (P32099)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     76     79       ATP     G-A-V-[FY]  
BINDING     71     71       ATP     R  
BINDING     134     134       ATP     K  
BINDING     134     134       Lipoate     K  

Additional information [?]

Size range 337-339 amino acids
Related rules None
Fusion None


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