HAMAP rule MF_01611
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01611 |
| Accession | MF_01611 |
| Dates | 13-JUN-2005 (Created)
08-OCT-2024 (Last updated, Version 29) |
| Name | FO_synth_sub1 |
| Scope(s) |
Bacteria Cyanobacteriota Archaea Methanobacteriati |
| Template(s) | Q57888; [ Recover all ] |
| Triggered by |
HAMAP; MF_01611 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | COFG |
| Protein name | RecName: Full=7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase; EC=4.3.1.32; AltName: Full=FO synthase subunit 1; |
| Gene name | Name=cofG; |
Comments
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| FUNCTION | Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8- hydroxy-5-deazariboflavin (FO) from 5-amino-5-(4-hydroxybenzyl)-6-(D- ribitylimino)-5,6-dihydrouracil. |
| CATALYTIC ACTIVITY | Reaction=5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6- dihydrouracil + S-adenosyl-L-methionine = 7,8-didemethyl-8-hydroxy-5- deazariboflavin + 5'-deoxyadenosine + L-methionine + NH4(+) + H(+); Xref=Rhea:RHEA:55204, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:28938, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:59904, ChEBI:CHEBI:85936; EC=4.3.1.32; |
| case <FTGroup:1> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
| end case | |
| PATHWAY | Cofactor biosynthesis; coenzyme F0 biosynthesis. |
| SUBUNIT | The FO synthase complex consists of two subunits, CofG and CofH. |
| SIMILARITY | Belongs to the radical SAM superfamily. CofG family. |
Keywords
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| case <FTGroup:1> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| S-adenosyl-L-methionine | |
| end case | |
| Lyase | |
Gene Ontology
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| case <FTGroup:1> | |
| GO:0005506; Molecular function:iron ion binding | |
| end case | |
| GO:0016841; Molecular function:ammonia-lyase activity | |
Cross-references
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Features
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| From: COFG_METJA (Q57888) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 49 | 49 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
| BINDING | 53 | 53 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
| BINDING | 56 | 56 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_note="4Fe-4S-S-AdoMet" |
C | 1 | |||||||
Additional information
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| Size range | 313-380 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: MF_01612 (cofH) |
| Comments | Euryarchaeota (Methanobacteriati), cyanobacteria and actinomycetes are known to produce coenzyme FO. Euryarchaeota and actinomycetes use it to synthesize coenzyme F420, whereas cyanobacteria use it for FO-dependent DNA photolyases. In actinomycetes, the two subunits cofG and cofH are fused, and are called fbiC. Possible wrong starts. |