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Annotation rule MF_01621
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General rule information [?]

Accession MF_01621
Dates 18-NOV-2005 (Created)
8-NOV-2018 (Last updated, Version 27)
Name FadB
Scope
Bacteria; Gammaproteobacteria
Templates P21177 (FADB_ECOLI); P28793 (FADB_PSEFR): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
FADB
Protein name
RecName: Full=Fatty acid oxidation complex subunit alpha;
Includes:
RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase;
EC=4.2.1.17;
EC=5.1.2.3;
EC=5.3.3.8;
Includes:
RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
EC=1.1.1.35;
Gene name
fadB

Comments [?]

Function Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.
Catalytic activity Reaction=a (3S)-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;.
Reaction=a (3S)-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318, ChEBI:CHEBI:58856; EC=4.2.1.17;.
Reaction=a 4-saturated-(3S)-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521, ChEBI:CHEBI:137480; EC=4.2.1.17;.
Reaction=(3S)-hydroxybutanoyl-CoA = (3R)-hydroxybutanoyl-CoA; Xref=Rhea:RHEA:21760, ChEBI:CHEBI:57315, ChEBI:CHEBI:57316; EC=5.1.2.3;.
Reaction=a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA; Xref=Rhea:RHEA:45900, ChEBI:CHEBI:85097, ChEBI:CHEBI:85489; EC=5.3.3.8;.
Reaction=a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA; Xref=Rhea:RHEA:45228, ChEBI:CHEBI:58521, ChEBI:CHEBI:85097; EC=5.3.3.8;.
Pathway Lipid metabolism; fatty acid beta-oxidation.
Subunit Heterotetramer of two alpha chains (FadB) and two beta chains (FadA).
Similarity In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Keywords [?]


Gene Ontology [?]

GO:0003857; Molecular function: 3-hydroxyacyl-CoA dehydrogenase activity.
GO:0004165; Molecular function: dodecenoyl-CoA delta-isomerase activity.
GO:0004300; Molecular function: enoyl-CoA hydratase activity.
GO:0008692; Molecular function: 3-hydroxybutyryl-CoA epimerase activity.
GO:0006631; Biological process: fatty acid metabolic process.
GO:0016042; Biological process: lipid catabolic process.

Cross-references [?]

PROSITE PS00067; 3HCDH; 1;
PS00166; ENOYL_COA_HYDRATASE; 1;
Pfam PF00725; 3HCDH; 1;
PF02737; 3HCDH_N; 1;
PF00378; ECH; 1;
TIGRFAMs TIGR02437; FadB; 1;

Features [?]

From: FADB_PSEFR (P28793)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     401     403       NAD     [VI]-[VT]-E  
REGION     Nter     190       Enoyl-CoA hydratase/isomerase        
REGION     312     Cter       3-hydroxyacyl-CoA dehydrogenase        
ACT_SITE     451     451       For 3-hydroxyacyl-CoA dehydrogenase activity     H  
BINDING     297     297       Substrate     D  
BINDING     325     325       NAD; via amide nitrogen     M  
BINDING     344     344       NAD     D  
BINDING     408     408       NAD     K  
BINDING     430     430       NAD     S  
BINDING     454     454       NAD     N  
BINDING     501     501       Substrate     N  
BINDING (Optional)     660     660       Substrate     Y  
SITE     120     120       Important for catalytic activity     E  
SITE     140     140       Important for catalytic activity     E  

Additional information [?]

Size range 715-729 amino acids
Related rules None
Fusion None