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HAMAP rule MF_01621

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General rule information [?]

Accession MF_01621
Dates 18-NOV-2005 (Created)
7-MAY-2024 (Last updated, Version 36)
Name FadB
Scope(s) Bacteria
Gammaproteobacteria
Template(s) P21177 (FADB_ECOLI); P28793 (FADB_PSEFR); [ Recover all ]
Triggered by HAMAP; MF_01621 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier FADB
Protein name RecName: Full=Fatty acid oxidation complex subunit alpha;
                 Includes:
RecName: Full=Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase;
                 EC=4.2.1.17;
                 EC=5.1.2.3;
                 EC=5.3.3.8;
                 Includes:
RecName: Full=3-hydroxyacyl-CoA dehydrogenase;
                 EC=1.1.1.35;
Gene name Name=fadB;

Comments [?]

FUNCTIONInvolved in the aerobic and anaerobic degradation of long- chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate.
CATALYTIC ACTIVITY Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) + NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CATALYTIC ACTIVITY Reaction=a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:16105, ChEBI:CHEBI:15377, ChEBI:CHEBI:57318, ChEBI:CHEBI:58856; EC=4.2.1.17;
CATALYTIC ACTIVITY Reaction=a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O; Xref=Rhea:RHEA:20724, ChEBI:CHEBI:15377, ChEBI:CHEBI:58521, ChEBI:CHEBI:137480; EC=4.2.1.17;
CATALYTIC ACTIVITY Reaction=(3S)-3-hydroxybutanoyl-CoA = (3R)-3-hydroxybutanoyl-CoA; Xref=Rhea:RHEA:21760, ChEBI:CHEBI:57315, ChEBI:CHEBI:57316; EC=5.1.2.3;
CATALYTIC ACTIVITY Reaction=a (3Z)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA; Xref=Rhea:RHEA:45900, ChEBI:CHEBI:85097, ChEBI:CHEBI:85489; EC=5.3.3.8;
CATALYTIC ACTIVITY Reaction=a (3E)-enoyl-CoA = a 4-saturated (2E)-enoyl-CoA; Xref=Rhea:RHEA:45228, ChEBI:CHEBI:58521, ChEBI:CHEBI:85097; EC=5.3.3.8;
PATHWAYLipid metabolism; fatty acid beta-oxidation.
SUBUNITHeterotetramer of two alpha chains (FadB) and two beta chains (FadA).
SIMILARITYIn the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
SIMILARITYIn the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Keywords [?]

Fatty acid metabolism
Isomerase
Lipid degradation
Lipid metabolism
Lyase
Multifunctional enzyme
NAD
Oxidoreductase

Gene Ontology [?]

GO:0003857; Molecular function:3-hydroxyacyl-CoA dehydrogenase activity
GO:0004165; Molecular function:delta(3)-delta(2)-enoyl-CoA isomerase activity
GO:0004300; Molecular function:enoyl-CoA hydratase activity
GO:0008692; Molecular function:3-hydroxybutyryl-CoA epimerase activity
GO:0006635; Biological process:fatty acid beta-oxidation
GO:0016042; Biological process:lipid catabolic process

Cross-references [?]

PROSITE PS00067; 3HCDH; 1;
PROSITE PS00166; ENOYL_COA_HYDRATASE; 1;
Pfam PF00725; 3HCDH; 1;
Pfam PF02737; 3HCDH_N; 1;
Pfam PF00378; ECH; 1;
NCBIfam TIGR02437; FadB; 1;

Features [?]

From: FADB_PSEFR (P28793)
Key From To Description Tag Condition FTGroup
BINDING 401 403 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 [VI]-[VT]-E
REGION Nter 190 /note="Enoyl-CoA hydratase/isomerase"
REGION 312 Cter /note="3-hydroxyacyl-CoA dehydrogenase"
ACT_SITE 451 451 /note="For 3-hydroxyacyl-CoA dehydrogenase activity" H
BINDING 297 297 /ligand="substrate" D
BINDING 325 325 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 M
BINDING 344 344 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 D
BINDING 408 408 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 K
BINDING 430 430 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 S
BINDING 454 454 /ligand="NAD(+)"
/ligand_id="ChEBI:CHEBI:57540"		 N
BINDING 501 501 /ligand="substrate" N
BINDING 660 660 /ligand="substrate" Y
SITE 120 120 /note="Important for catalytic activity" E
SITE 140 140 /note="Important for catalytic activity" E

Additional information [?]

Size range 715-729 amino acids
Related rules None
Fusion Nter: None Cter: None



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