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HAMAP rule MF_01622

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_01622
Accession MF_01622
Dates 21-NOV-2005 (Created)
03-SEP-2024 (Last updated, Version 26)
Name tRNA_sel_U_synth
Scope(s) Bacteria
Pseudomonadota
Template(s) P33667; Q8ZR88; [ Recover all ]
Triggered by HAMAP; MF_01622 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SELU
Protein name RecName: Full=tRNA 2-selenouridine synthase;
                 EC=2.9.1.3;
Gene name Name=selU;

Comments [?]

FUNCTIONInvolved in the post-transcriptional modification of the uridine at the wobble position (U34) of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Catalyzes the conversion of 2-thiouridine (S2U-RNA) to 2- selenouridine (Se2U-RNA). Acts in a two-step process involving geranylation of 2-thiouridine (S2U) to S-geranyl-2-thiouridine (geS2U) and subsequent selenation of the latter derivative to 2-selenouridine (Se2U) in the tRNA chain.
CATALYTIC ACTIVITY Reaction=5-methylaminomethyl-2-thiouridine(34) in tRNA + selenophosphate + (2E)-geranyl diphosphate + H2O + H(+) = 5- methylaminomethyl-2-selenouridine(34) in tRNA + (2E)-thiogeraniol + phosphate + diphosphate; Xref=Rhea:RHEA:42716, Rhea:RHEA-COMP:10195, Rhea:RHEA-COMP:10196, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16144, ChEBI:CHEBI:33019, ChEBI:CHEBI:43474, ChEBI:CHEBI:58057, ChEBI:CHEBI:74455, ChEBI:CHEBI:82743, ChEBI:CHEBI:143703; EC=2.9.1.3; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:42717;
CATALYTIC ACTIVITY Reaction=5-methylaminomethyl-2-thiouridine(34) in tRNA + (2E)-geranyl diphosphate = 5-methylaminomethyl-S-(2E)-geranyl-thiouridine(34) in tRNA + diphosphate; Xref=Rhea:RHEA:14085, Rhea:RHEA-COMP:10195, Rhea:RHEA-COMP:14654, ChEBI:CHEBI:33019, ChEBI:CHEBI:58057, ChEBI:CHEBI:74455, ChEBI:CHEBI:140632; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14086;
CATALYTIC ACTIVITY Reaction=5-methylaminomethyl-S-(2E)-geranyl-thiouridine(34) in tRNA + selenophosphate + H(+) = 5-methylaminomethyl-2-(Se- phospho)selenouridine(34) in tRNA + (2E)-thiogeraniol; Xref=Rhea:RHEA:60172, Rhea:RHEA-COMP:14654, Rhea:RHEA-COMP:15523, ChEBI:CHEBI:15378, ChEBI:CHEBI:16144, ChEBI:CHEBI:140632, ChEBI:CHEBI:143702, ChEBI:CHEBI:143703; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60173;
CATALYTIC ACTIVITY Reaction=5-methylaminomethyl-2-(Se-phospho)selenouridine(34) in tRNA + H2O = 5-methylaminomethyl-2-selenouridine(34) in tRNA + phosphate; Xref=Rhea:RHEA:60176, Rhea:RHEA-COMP:10196, Rhea:RHEA-COMP:15523, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:82743, ChEBI:CHEBI:143702; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60177;
SUBUNITMonomer.
SIMILARITYBelongs to the SelU family.

Keywords [?]

Selenium
Transferase

Gene Ontology [?]

GO:0016765; Molecular function:transferase activity, transferring alkyl or aryl (other than methyl) groups
GO:0002098; Biological process:tRNA wobble uridine modification

Cross-references [?]

PROSITE PS50206; RHODANESE_3; 1;
NCBIfam TIGR03167; TRNA_sel_U_synt; 1;

Features [?]

From: SELU_ECOLI (P33667)
Key From To Description Tag Condition FTGroup
ACT_SITE 97 97 /note="S-selanylcysteine intermediate" C

Additional information [?]

Size range 350-410 amino acids
Related rules None
Fusion Nter: None Cter: None



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