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HAMAP rule MF_01623

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General rule information [?]

Accession MF_01623
Dates 23-NOV-2005 (Created)
19-NOV-2022 (Last updated, Version 19)
Name MepA
Bacteria; Enterobacterales
Template P0C0T5 (MEPA_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Penicillin-insensitive murein endopeptidase;
EC 3.4.24.-;
AltName: Full=D-alanyl-D-alanine-endopeptidase;
Flags: Precursor;
Gene name

Comments [?]

Function Murein endopeptidase that cleaves the D-alanyl-meso-2,6-diamino-pimelyl amide bond that connects peptidoglycan strands. Likely plays a role in the removal of murein from the sacculus.
Cofactor Zn(2+)
Note: Binds 2 Zn(2+) ions per subunit. Zn(2+) ion 1 is bound in the active site. Zn(2+) ion 2 is bound at the dimer interface by residues from both subunits.
Subunit Dimer.
Subcellular location Periplasm.
Similarity Belongs to the peptidase M74 family.

Keywords [?]

case <FT:7> or <FT:8> or <FT:9>
end case

Gene Ontology [?]

GO:0004222; Molecular function: metalloendopeptidase activity.
GO:0000270; Biological process: peptidoglycan metabolic process.
GO:0042597; Cellular component: periplasmic space.

Cross-references [?]

Pfam PF03411; Peptidase_M74; 1;
PIRSF PIRSF018455; MepA; 1;

Computed features [?]

General Signal; -; 1; trigger=yes;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
BINDING     110     110       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
BINDING     113     113       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
BINDING     120     120       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     D  
BINDING     147     147       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     D  
BINDING     150     150       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2     H  
BINDING     211     211       /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1     H  
DISULFID     44     265             C-x*-C  
DISULFID     187     235             C-x*-C  
DISULFID     216     223             C-x*-C  

Additional information [?]

Size range 274-281 amino acids
Related rules None
Fusion None