HAMAP rule MF_01623

General rule information [?]

Accession	MF_01623
Dates	23-NOV-2005 (Created) 19-NOV-2022 (Last updated, Version 19)

Name

MepA

Scope

Bacteria; Enterobacterales

Template

P0C0T5 (MEPA_ECOLI)

Triggered by

HAMAP; MF_01623 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MEPA

Protein name

RecName:		Full=Penicillin-insensitive murein endopeptidase;
		EC 3.4.24.-;
AltName:		Full=D-alanyl-D-alanine-endopeptidase;
		Short=DD-endopeptidase;
Flags:		Precursor;

Gene name

mepA

Comments [?]

Function	Murein endopeptidase that cleaves the D-alanyl-meso-2,6-diamino-pimelyl amide bond that connects peptidoglycan strands. Likely plays a role in the removal of murein from the sacculus.
Cofactor	Zn(2+) Note: Binds 2 Zn(2+) ions per subunit. Zn(2+) ion 1 is bound in the active site. Zn(2+) ion 2 is bound at the dimer interface by residues from both subunits.
Subunit	Dimer.
Subcellular location	Periplasm.
Similarity	Belongs to the peptidase M74 family.

Keywords [?]

case <FT:7> or <FT:8> or <FT:9>

Disulfide bond

end case

Hydrolase
Metal-binding
Metalloprotease
Periplasm
Protease
Signal
Zinc

Gene Ontology [?]

GO:0004222; Molecular function: metalloendopeptidase activity.
GO:0000270; Biological process: peptidoglycan metabolic process.
GO:0042597; Cellular component: periplasmic space.

Cross-references [?]

Pfam	PF03411; Peptidase_M74; 1;
PIRSF	PIRSF018455; MepA; 1;

Computed features [?]

General

Signal; -; 1; trigger=yes;

Features [?]

From: MEPA_ECOLI (P0C0T5)

Key From To Description Tag Condition FTGroup

BINDING 110 110 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

BINDING 113 113 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

BINDING 120 120 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 D

BINDING 147 147 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 D

BINDING 150 150 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2 H

BINDING 211 211 /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1 H

DISULFID 44 265 C-x*-C

DISULFID 187 235 C-x*-C

DISULFID 216 223 C-x*-C

Additional information [?]

Size range	274-281 amino acids
Related rules	None
Fusion	None

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