HAMAP rule MF_01623
General rule information
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| Accession | MF_01623 |
| Dates | 23-NOV-2005 (Created)
19-NOV-2022 (Last updated, Version 19) |
| Name | MepA |
| Scope(s) |
Bacteria Enterobacterales |
| Template(s) | P0C0T5 (MEPA_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01623 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MEPA |
| Protein name | RecName: Full=Penicillin-insensitive murein endopeptidase; EC=3.4.24.-; AltName: Full=D-alanyl-D-alanine-endopeptidase; Short=DD-endopeptidase; Flags: Precursor; |
| Gene name | Name=mepA; |
Comments
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| FUNCTION | Murein endopeptidase that cleaves the D-alanyl-meso-2,6- diamino-pimelyl amide bond that connects peptidoglycan strands. Likely plays a role in the removal of murein from the sacculus. |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per subunit. Zn(2+) ion 1 is bound in the active site. Zn(2+) ion 2 is bound at the dimer interface by residues from both subunits.; |
| SUBUNIT | Dimer. |
| SUBCELLULAR LOCATION | Periplasm. |
| SIMILARITY | Belongs to the peptidase M74 family. |
Keywords
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| case <FT:7> or <FT:8> or <FT:9> | |
| Disulfide bond | |
| end case | |
| Hydrolase | |
| Metal-binding | |
| Metalloprotease | |
| Periplasm | |
| Protease | |
| Signal | |
| Zinc | |
Gene Ontology
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| GO:0004222; Molecular function:metalloendopeptidase activity |
| GO:0000270; Biological process:peptidoglycan metabolic process |
| GO:0042597; Cellular component:periplasmic space |
Cross-references
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| Pfam | PF03411; Peptidase_M74; 1; |
| PIRSF | PIRSF018455; MepA; 1; |
| General | Signal; -; 1; |
Features
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| From: MEPA_ECOLI (P0C0T5) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 110 | 110 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
H | ||||||||
| BINDING | 113 | 113 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
H | ||||||||
| BINDING | 120 | 120 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
D | ||||||||
| BINDING | 147 | 147 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
D | ||||||||
| BINDING | 150 | 150 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="2" |
H | ||||||||
| BINDING | 211 | 211 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_label="1" |
H | ||||||||
| DISULFID | 44 | 265 | C-x*-C | |||||||||
| DISULFID | 187 | 235 | C-x*-C | |||||||||
| DISULFID | 216 | 223 | C-x*-C | |||||||||
Additional information
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| Size range | 274-281 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |