HAMAP rule MF_01627
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01627 |
| Accession | MF_01627 |
| Dates | 13-DEC-2005 (Created)
3-SEP-2024 (Last updated, Version 26) |
| Name | Pur_nucleosid_phosp |
| Scope(s) |
Bacteria Bacillota Pseudomonadota |
| Template(s) | P0ABP8 (DEOD_ECOLI); Q8ZJV7 (DEOD_SALTY); [ Recover all ] |
| Triggered by |
HAMAP; MF_01627 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DEOD |
| Protein name | RecName: Full=Purine nucleoside phosphorylase DeoD-type; Short=PNP; EC=2.4.2.1; |
| Gene name | Name=deoD; |
Comments
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| FUNCTION | Catalyzes the reversible phosphorolytic breakdown of the N- glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1- phosphate. |
| CATALYTIC ACTIVITY | Reaction=a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate; Xref=Rhea:RHEA:19805, ChEBI:CHEBI:26386, ChEBI:CHEBI:43474, ChEBI:CHEBI:57720, ChEBI:CHEBI:142355; EC=2.4.2.1; |
| CATALYTIC ACTIVITY | Reaction=a purine 2'-deoxy-D-ribonucleoside + phosphate = a purine nucleobase + 2-deoxy-alpha-D-ribose 1-phosphate; Xref=Rhea:RHEA:36431, ChEBI:CHEBI:26386, ChEBI:CHEBI:43474, ChEBI:CHEBI:57259, ChEBI:CHEBI:142361; EC=2.4.2.1; |
| SUBUNIT | Homohexamer; trimer of homodimers. |
| SIMILARITY | Belongs to the PNP/UDP phosphorylase family. |
Keywords
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| case <FTTag:acetylation> | |
| Acetylation | |
| end case | |
| Glycosyltransferase | |
| Transferase | |
Gene Ontology
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| GO:0004731; Molecular function:purine-nucleoside phosphorylase activity |
| GO:0042278; Biological process:purine nucleoside metabolic process |
Cross-references
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Features
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| From: DEOD_ECOLI (P0ABP8) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 205 | 205 | /note="Proton donor" | D | ||||||||
| SITE | 218 | 218 | /note="Important for catalytic activity" | R | ||||||||
| BINDING | 88 | 91 | /ligand="phosphate" /ligand_id="ChEBI:CHEBI:43474" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
R-[IV]-G-[TS] | ||||||||
| BINDING | 180 | 182 | /ligand="a purine D-ribonucleoside" /ligand_id="ChEBI:CHEBI:142355" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
[ED]-M-E | ||||||||
| BINDING | 204 | 205 | /ligand="a purine D-ribonucleoside" /ligand_id="ChEBI:CHEBI:142355" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
[ST]-[DN] | ||||||||
| BINDING | 5 | 5 | /ligand="a purine D-ribonucleoside" /ligand_id="ChEBI:CHEBI:142355" /ligand_note="ligand shared between dimeric partners" |
H | ||||||||
| BINDING | 21 | 21 | /ligand="phosphate" /ligand_id="ChEBI:CHEBI:43474" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
G | ||||||||
| BINDING | 25 | 25 | /ligand="phosphate" /ligand_id="ChEBI:CHEBI:43474" /ligand_note="ligand shared between dimeric partners" /note="in other chain" |
R | ||||||||
| BINDING | 44 | 44 | /ligand="phosphate" /ligand_id="ChEBI:CHEBI:43474" /ligand_note="ligand shared between dimeric partners" |
R | ||||||||
| case <OC:Escherichia> or <OC:Shigella> | ||||||||||||
| MOD_RES | 27 | 27 | /note="N6-acetyllysine" | acetylation | K | |||||||
| end case | ||||||||||||
Additional information
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| Size range | 220-270 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |