HAMAP rule MF_01629
General rule information
[?]
| PURL | https://purl.expasy.org/hamap/rule/MF_01629 |
| Accession | MF_01629 |
| Dates | 14-DEC-2005 (Created)
03-SEP-2024 (Last updated, Version 38) |
| Name | PdxH |
| Scope(s) |
Bacteria Actinomycetota Bacteroidota Cyanobacteriota Deinococcota Planctomycetota Pseudomonadota Spirochaetota |
| Template(s) | P0AFI7; P9WIJ1; [ Recover all ] |
| Triggered by |
HAMAP; MF_01629 (Get profile general information and statistics) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
| Identifier | PDXH |
| Protein name | RecName: Full=Pyridoxine/pyridoxamine 5'-phosphate oxidase; EC=1.4.3.5; AltName: Full=PNP/PMP oxidase; Short=PNPOx; AltName: Full=Pyridoxal 5'-phosphate synthase; |
| Gene name | Name=pdxH; |
Comments
[?]
| FUNCTION | Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP). |
| CATALYTIC ACTIVITY | Reaction=pyridoxamine 5'-phosphate + O2 + H2O = pyridoxal 5'-phosphate + H2O2 + NH4(+); Xref=Rhea:RHEA:15817, ChEBI:CHEBI:15377, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:28938, ChEBI:CHEBI:58451, ChEBI:CHEBI:597326; EC=1.4.3.5; |
| CATALYTIC ACTIVITY | Reaction=pyridoxine 5'-phosphate + O2 = pyridoxal 5'-phosphate + H2O2; Xref=Rhea:RHEA:15149, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:58589, ChEBI:CHEBI:597326; EC=1.4.3.5; |
| COFACTOR | Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per subunit.; |
| PATHWAY | Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. |
| PATHWAY | Cofactor metabolism; pyridoxal 5'-phosphate salvage; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the pyridoxamine 5'-phosphate oxidase family. |
Keywords
[?]
Gene Ontology
[?]
| GO:0004733; Molecular function:pyridoxamine phosphate oxidase activity |
| GO:0010181; Molecular function:FMN binding |
| GO:0042816; Biological process:vitamin B6 metabolic process |
Cross-references
[?]
Features
[?]
| From: PDXH_ECOLI (P0AFI7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 67 | 72 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
R-x-x-L-x-[KR] | ||||||||
| BINDING | 82 | 83 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
[YF]-[TS] | ||||||||
| BINDING | 146 | 147 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
Q-S | ||||||||
| BINDING | 14 | 17 | /ligand="substrate" | R-x-x-Y | ||||||||
| BINDING | 197 | 199 | /ligand="substrate" | R-x-[HN] | ||||||||
| BINDING | 72 | 72 | /ligand="substrate" | [KR] | ||||||||
| BINDING | 88 | 88 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
[RK] | ||||||||
| BINDING | 89 | 89 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
K | ||||||||
| BINDING | 111 | 111 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
Q | ||||||||
| BINDING | 129 | 129 | /ligand="substrate" | Y | ||||||||
| BINDING | 133 | 133 | /ligand="substrate" | R | ||||||||
| BINDING | 137 | 137 | /ligand="substrate" | S | ||||||||
| BINDING | 191 | 191 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
W | ||||||||
| BINDING | 201 | 201 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
R | ||||||||
Additional information
[?]
| Size range | 192-267 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | In M.tuberculosis, the enzyme catalyzes only the oxidation of pyridoxine 5'-phosphate (PNP), it does not recognize pyridoxamine 5'-phosphate (PMP) as a substrate (PubMed:22110704). |