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Annotation rule MF_01631
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General rule information [?]

Accession MF_01631
Dates 4-APR-2006 (Created)
19-NOV-2019 (Last updated, Version 36)
Name GlmU
Scope
Bacteria
Templates P0ACC7 (GLMU_ECOLI); Q97R46 (GLMU_STRPN); P9WMN3 (GLMU_MYCTU): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
GLMU
Protein name
RecName: Full=Bifunctional protein GlmU;
Includes:
RecName: Full=UDP-N-acetylglucosamine pyrophosphorylase;
EC 2.7.7.23;
AltName: Full=N-acetylglucosamine-1-phosphate uridyltransferase;
Includes:
RecName: Full=Glucosamine-1-phosphate N-acetyltransferase;
EC 2.3.1.157;
Gene name
glmU

Comments [?]

Function Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.
Catalytic activity RHEA:13725: acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate
EC 2.3.1.157
RHEA:13509: H(+) + N-acetyl-alpha-D-glucosamine 1-phosphate + UTP = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
EC 2.7.7.23
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
Pathway Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.
Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.
Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.
Subunit Homotrimer.
Subcellular location Cytoplasm.
Similarity In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.
In the C-terminal section; belongs to the transferase hexapeptide repeat family.

Keywords [?]

Cytoplasm
Acyltransferase
Cell shape
Cell wall biogenesis/degradation
Magnesium
Metal-binding
Multifunctional enzyme
Nucleotidyltransferase
Peptidoglycan synthesis
Repeat
Transferase

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0003977; Molecular function: UDP-N-acetylglucosamine diphosphorylase activity.
GO:0019134; Molecular function: glucosamine-1-phosphate N-acetyltransferase activity.
GO:0000902; Biological process: cell morphogenesis.
GO:0009252; Biological process: peptidoglycan biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS00101; HEXAPEP_TRANSFERASES; 1;
Pfam PF00132; Hexapep; 7;
PF00483; NTP_transferase; 1;
PF01128; IspD; 1;
PF02348; CTP_transf_3; 1;
PRINTS PR01590; HTHFIS; 1;
PR01415; ANKYRIN; 1;
TIGRFAMs TIGR01173; GlmU; 1;

Features [?]

From: GLMU_ECOLI (P0ACC7)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     Nter     229       Pyrophosphorylase        
REGION (Optional)     11     14       UDP-GlcNAc binding     L-A-A-G  
REGION (Optional)     81     82       UDP-GlcNAc binding     G-T  
REGION (Optional)     103     105       UDP-GlcNAc binding     [SY]-G-D  
REGION     230     250       Linker        
REGION     251     Cter       N-acetyltransferase        
REGION (Optional)     386     387       Acetyl-CoA binding     N-Y  
ACT_SITE     363     363       Proton acceptor     H  
METAL     105     105       Magnesium     D  
METAL     227     227       Magnesium     N  
BINDING (Optional)     25     25       UDP-GlcNAc     K  
BINDING (Optional)     76     76       UDP-GlcNAc     Q  
BINDING (Optional)     140     140       UDP-GlcNAc; via amide nitrogen     G  
BINDING (Optional)     154     154       UDP-GlcNAc     E  
BINDING (Optional)     169     169       UDP-GlcNAc     N  
BINDING (Optional)     227     227       UDP-GlcNAc     N  
BINDING (Optional)     333     333       UDP-GlcNAc     R  
BINDING (Optional)     351     351       UDP-GlcNAc     K  
BINDING (Optional)     366     366       UDP-GlcNAc     Y  
BINDING (Optional)     377     377       UDP-GlcNAc     N  
BINDING (Optional)     380     380       Acetyl-CoA; via amide nitrogen     A  
BINDING (Optional)     405     405       Acetyl-CoA     S  
BINDING (Optional)     423     423       Acetyl-CoA; via amide nitrogen     [ACGST]  
BINDING (Optional)     440     440       Acetyl-CoA     R  

Additional information [?]

Size range 339-500 amino acids
Related rules None
Fusion Nter: None; Cter: <PPC domain>
Comments SYNJA and SYNJB are fusioned in their C-terminal parts to a PPC domain.


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