HAMAP rule MF_01644
General rule information
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| Accession | MF_01644 |
| Dates | 14-JAN-2008 (Created)
1-JUN-2023 (Last updated, Version 19) |
| Name | D_hydantoinase |
| Scope(s) |
Bacteria Enterobacteriaceae |
| Template(s) | Q46806 (PHYDA_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01644 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PHYDA |
| Protein name | RecName: Full=D-phenylhydantoinase; EC=3.5.2.-; AltName: Full=Hydantoin-utilizing enzyme HyuA; |
| Gene name | Name=hyuA; |
Comments
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| FUNCTION | Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives with an aromatic side chains at the 5'- position. Has no activity on dihydropyrimidines. The physiological function is unknown. |
| CATALYTIC ACTIVITY | Reaction=D-5-phenylhydantoin + H2O = H(+) + N-carbamoyl-D- phenylglycine; Xref=Rhea:RHEA:51664, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:140750, ChEBI:CHEBI:140758; |
| COFACTOR | Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Note=Binds 2 divalent metal cations per subunit.; |
| SUBUNIT | Homotetramer. |
| PTM | Carboxylation allows a single lysine to coordinate two divalent metal cations. |
| SIMILARITY | Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family. |
Keywords
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Gene Ontology
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| GO:0016812; Molecular function:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides |
Cross-references
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Features
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| From: PHYDA_ECOLI (Q46806) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 59 | 59 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1" |
H | ||||||||
| BINDING | 61 | 61 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1" |
H | ||||||||
| BINDING | 151 | 151 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1" /note="via carbamate group" |
K | ||||||||
| BINDING | 151 | 151 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /note="via carbamate group" |
K | ||||||||
| BINDING | 182 | 182 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" |
H | ||||||||
| BINDING | 239 | 239 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" |
H | ||||||||
| BINDING | 313 | 313 | /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1" |
D | ||||||||
| BINDING | 156 | 156 | /ligand="substrate" | Y | ||||||||
| BINDING | 286 | 286 | /ligand="substrate" | S | ||||||||
| BINDING | 335 | 335 | /ligand="substrate" | N | ||||||||
| MOD_RES | 151 | 151 | /note="N6-carboxylysine" | K | ||||||||
Additional information
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| Size range | 461-461 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |