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HAMAP rule MF_01644

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General rule information [?]

Accession MF_01644
Dates 14-JAN-2008 (Created)
1-JUN-2023 (Last updated, Version 19)
Name D_hydantoinase
Scope(s) Bacteria
Enterobacteriaceae
Template(s) Q46806 (PHYDA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01644 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PHYDA
Protein name RecName: Full=D-phenylhydantoinase;
                 EC=3.5.2.-;
AltName: Full=Hydantoin-utilizing enzyme HyuA;
Gene name Name=hyuA;

Comments [?]

FUNCTIONCatalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives with an aromatic side chains at the 5'- position. Has no activity on dihydropyrimidines. The physiological function is unknown.
CATALYTIC ACTIVITY Reaction=D-5-phenylhydantoin + H2O = H(+) + N-carbamoyl-D- phenylglycine; Xref=Rhea:RHEA:51664, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:140750, ChEBI:CHEBI:140758;
COFACTOR Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Note=Binds 2 divalent metal cations per subunit.;
SUBUNITHomotetramer.
PTMCarboxylation allows a single lysine to coordinate two divalent metal cations.
SIMILARITYBelongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.

Keywords [?]


Gene Ontology [?]

GO:0016812; Molecular function:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides

Cross-references [?]

Pfam PF01979; Amidohydro_1; 1;
NCBIfam TIGR02033; D-hydantoinase; 1;

Features [?]

From: PHYDA_ECOLI (Q46806)
Key From To Description Tag Condition FTGroup
BINDING 59 59 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_label="1"
H
BINDING 61 61 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_label="1"
H
BINDING 151 151 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_label="1"
/note="via carbamate group"
K
BINDING 151 151 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_label="2"
/note="via carbamate group"
K
BINDING 182 182 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_label="2"
H
BINDING 239 239 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_label="2"
H
BINDING 313 313 /ligand="a divalent metal cation"
/ligand_id="ChEBI:CHEBI:60240"
/ligand_label="1"
D
BINDING 156 156 /ligand="substrate" Y
BINDING 286 286 /ligand="substrate" S
BINDING 335 335 /ligand="substrate" N
MOD_RES 151 151 /note="N6-carboxylysine" K

Additional information [?]

Size range 461-461 amino acids
Related rules None
Fusion Nter: None Cter: None



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