HAMAP rule MF_01646

General rule information [?]

Accession	MF_01646
Dates	26-MAR-2008 (Created) 1-JUN-2023 (Last updated, Version 22)

Name	Siroheme_synth

Scope

Bacteria; Pseudomonadota

Templates

P0AEA8 (CYSG_ECOLI); P25924 (CYSG_SALTY): [Recover all]

Triggered by

HAMAP; MF_01646 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

CYSG

Protein name

RecName:		Full=Siroheme synthase;
Includes:
	RecName:		Full=Uroporphyrinogen-III C-methyltransferase;
			Short=Urogen III methylase;
			EC 2.1.1.107;
	AltName:		Full=SUMT;
	AltName:		Full=Uroporphyrinogen III methylase;
			Short=UROM;
Includes:
	RecName:		Full=Precorrin-2 dehydrogenase;
			EC 1.3.1.76;
Includes:
	RecName:		Full=Sirohydrochlorin ferrochelatase;
			EC 4.99.1.4;

Gene name

cysG

Comments [?]

Function	Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.
Catalytic activity	RHEA:32459: 2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) + precorrin-2 + 2 S-adenosyl-L-homocysteine EC 2.1.1.107
	RHEA:15613: NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin EC 1.3.1.76
	RHEA:24360: 2 H(+) + siroheme = Fe(2+) + sirohydrochlorin EC 4.99.1.4
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
	Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
	Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
	Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.
	Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Similarity	In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.
	In the C-terminal section; belongs to the precorrin methyltransferase family.

Keywords [?]

Cobalamin biosynthesis
Lyase
Methyltransferase
Multifunctional enzyme
NAD
Oxidoreductase

case <FT:13>

Phosphoprotein

end case

Porphyrin biosynthesis
S-adenosyl-L-methionine
Transferase

Gene Ontology [?]

GO:0004325; Molecular function: ferrochelatase activity.
GO:0004851; Molecular function: uroporphyrin-III C-methyltransferase activity.
GO:0043115; Molecular function: precorrin-2 dehydrogenase activity.
GO:0006779; Biological process: porphyrin-containing compound biosynthetic process.
GO:0009236; Biological process: cobalamin biosynthetic process.
GO:0019354; Biological process: siroheme biosynthetic process.

Cross-references [?]

PROSITE	PS00839; SUMT_1; 1;
	PS00840; SUMT_2; 1;
Pfam	PF00590; TP_methylase; 1;
NCBIfam	TIGR01469; CobA_cysG_Cterm; 1;
	TIGR01470; CysG_Nterm; 1;

Features [?]

From: CYSG_SALTY (P25924)

Key From To Description Tag Condition FTGroup

BINDING 22 23 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540

BINDING 43 44 /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540

REGION Nter 204 Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase

REGION 216 Cter Uroporphyrinogen-III C-methyltransferase

BINDING 301 303 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 G-G-D

BINDING 331 332 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 [TS]-A

ACT_SITE 248 248 Proton acceptor D

ACT_SITE 270 270 Proton donor K

BINDING 225 225 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 P

BINDING 306 306 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 [IVL]

BINDING 382 382 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 M

BINDING 411 411 /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789 [GA]

MOD_RES 128 128 Phosphoserine S

Additional information [?]

Size range	457-549 amino acids
Related rules	None
Fusion	None

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