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HAMAP rule MF_01646

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General rule information [?]

Accession MF_01646
Dates 26-MAR-2008 (Created)
1-JUN-2023 (Last updated, Version 22)
Name Siroheme_synth
Scope(s) Bacteria
Template(s) P0AEA8 (CYSG_ECOLI); P25924 (CYSG_SALTY); [ Recover all ]
Triggered by HAMAP; MF_01646 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier CYSG
Protein name RecName: Full=Siroheme synthase;
RecName: Full=Uroporphyrinogen-III C-methyltransferase;
                 Short=Urogen III methylase;
AltName: Full=SUMT;
AltName: Full=Uroporphyrinogen III methylase;
RecName: Full=Precorrin-2 dehydrogenase;
RecName: Full=Sirohydrochlorin ferrochelatase;
Gene name Name=cysG;

Comments [?]

FUNCTIONMultifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.
CATALYTIC ACTIVITY Reaction=2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) + precorrin-2 + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:32459, ChEBI:CHEBI:15378, ChEBI:CHEBI:57308, ChEBI:CHEBI:57856, ChEBI:CHEBI:58827, ChEBI:CHEBI:59789; EC=;
CATALYTIC ACTIVITY Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin; Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=;
CATALYTIC ACTIVITY Reaction=2 H(+) + siroheme = Fe(2+) + sirohydrochlorin; Xref=Rhea:RHEA:24360, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:58351, ChEBI:CHEBI:60052; EC=;
PATHWAYCofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
PATHWAYCofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
PATHWAYPorphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
PATHWAYPorphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.
PATHWAYPorphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
SIMILARITYIn the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.
SIMILARITYIn the C-terminal section; belongs to the precorrin methyltransferase family.

Keywords [?]

Gene Ontology [?]

GO:0004325; Molecular function:ferrochelatase activity
GO:0004851; Molecular function:uroporphyrin-III C-methyltransferase activity
GO:0043115; Molecular function:precorrin-2 dehydrogenase activity
GO:0006779; Biological process:porphyrin-containing compound biosynthetic process
GO:0009236; Biological process:cobalamin biosynthetic process
GO:0019354; Biological process:siroheme biosynthetic process

Cross-references [?]

PROSITE PS00839; SUMT_1; 1;
PROSITE PS00840; SUMT_2; 1;
Pfam PF00590; TP_methylase; 1;
NCBIfam TIGR01469; CobA_cysG_Cterm; 1;
NCBIfam TIGR01470; CysG_Nterm; 1;

Features [?]

From: CYSG_SALTY (P25924)
Key From To Description Tag Condition FTGroup
BINDING 22 23 /ligand="NAD(+)"
BINDING 43 44 /ligand="NAD(+)"
REGION Nter 204 /note="Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase"
REGION 216 Cter /note="Uroporphyrinogen-III C-methyltransferase"
BINDING 301 303 /ligand="S-adenosyl-L-methionine"
BINDING 331 332 /ligand="S-adenosyl-L-methionine"
ACT_SITE 248 248 /note="Proton acceptor" D
ACT_SITE 270 270 /note="Proton donor" K
BINDING 225 225 /ligand="S-adenosyl-L-methionine"
BINDING 306 306 /ligand="S-adenosyl-L-methionine"
BINDING 382 382 /ligand="S-adenosyl-L-methionine"
BINDING 411 411 /ligand="S-adenosyl-L-methionine"
MOD_RES 128 128 /note="Phosphoserine" S

Additional information [?]

Size range 457-549 amino acids
Related rules None
Fusion Nter: None Cter: None

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