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HAMAP rule MF_01646

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General rule information [?]

Accession MF_01646
Dates 26-MAR-2008 (Created)
1-JUN-2023 (Last updated, Version 22)
Name Siroheme_synth
Bacteria; Pseudomonadota
Templates P0AEA8 (CYSG_ECOLI); P25924 (CYSG_SALTY): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Siroheme synthase;
RecName: Full=Uroporphyrinogen-III C-methyltransferase;
Short=Urogen III methylase;
AltName: Full=SUMT;
AltName: Full=Uroporphyrinogen III methylase;
RecName: Full=Precorrin-2 dehydrogenase;
RecName: Full=Sirohydrochlorin ferrochelatase;
Gene name

Comments [?]

Function Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.
Catalytic activity RHEA:32459: 2 S-adenosyl-L-methionine + uroporphyrinogen III = H(+) + precorrin-2 + 2 S-adenosyl-L-homocysteine
RHEA:15613: NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin
RHEA:24360: 2 H(+) + siroheme = Fe(2+) + sirohydrochlorin
Pathway Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1.
Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Similarity In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.
In the C-terminal section; belongs to the precorrin methyltransferase family.

Keywords [?]

case <FT:13>
end case

Gene Ontology [?]

GO:0004325; Molecular function: ferrochelatase activity.
GO:0004851; Molecular function: uroporphyrin-III C-methyltransferase activity.
GO:0043115; Molecular function: precorrin-2 dehydrogenase activity.
GO:0006779; Biological process: porphyrin-containing compound biosynthetic process.
GO:0009236; Biological process: cobalamin biosynthetic process.
GO:0019354; Biological process: siroheme biosynthetic process.

Cross-references [?]

PROSITE PS00839; SUMT_1; 1;
PS00840; SUMT_2; 1;
Pfam PF00590; TP_methylase; 1;
NCBIfam TIGR01469; CobA_cysG_Cterm; 1;
TIGR01470; CysG_Nterm; 1;

Features [?]

From: CYSG_SALTY (P25924)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     22     23       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540        
BINDING     43     44       /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540        
REGION     Nter     204       Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase        
REGION     216     Cter       Uroporphyrinogen-III C-methyltransferase        
BINDING     301     303       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     G-G-D  
BINDING     331     332       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [TS]-A  
ACT_SITE     248     248       Proton acceptor     D  
ACT_SITE     270     270       Proton donor     K  
BINDING     225     225       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     P  
BINDING     306     306       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [IVL]  
BINDING     382     382       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     M  
BINDING     411     411       /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789     [GA]  
MOD_RES     128     128       Phosphoserine     S  

Additional information [?]

Size range 457-549 amino acids
Related rules None
Fusion None