HAMAP rule MF_01646
General rule information
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| Accession | MF_01646 |
| Dates | 26-MAR-2008 (Created)
3-SEP-2024 (Last updated, Version 23) |
| Name | Siroheme_synth |
| Scope(s) |
Bacteria Pseudomonadota |
| Template(s) | P0AEA8 (CYSG_ECOLI); P25924 (CYSG_SALTY); [ Recover all ] |
| Triggered by |
HAMAP; MF_01646 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | CYSG |
| Protein name | RecName: Full=Siroheme synthase; Includes: RecName: Full=Uroporphyrinogen-III C-methyltransferase; Short=Urogen III methylase; EC=2.1.1.107; AltName: Full=SUMT; AltName: Full=Uroporphyrinogen III methylase; Short=UROM; Includes: RecName: Full=Precorrin-2 dehydrogenase; EC=1.3.1.76; Includes: RecName: Full=Sirohydrochlorin ferrochelatase; EC=4.99.1.4; |
| Gene name | Name=cysG; |
Comments
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| FUNCTION | Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme. |
| CATALYTIC ACTIVITY | Reaction=uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:32459, ChEBI:CHEBI:15378, ChEBI:CHEBI:57308, ChEBI:CHEBI:57856, ChEBI:CHEBI:58827, ChEBI:CHEBI:59789; EC=2.1.1.107; |
| CATALYTIC ACTIVITY | Reaction=precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+); Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76; |
| CATALYTIC ACTIVITY | Reaction=siroheme + 2 H(+) = sirohydrochlorin + Fe(2+); Xref=Rhea:RHEA:24360, ChEBI:CHEBI:15378, ChEBI:CHEBI:29033, ChEBI:CHEBI:58351, ChEBI:CHEBI:60052; EC=4.99.1.4; |
| PATHWAY | Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. |
| PATHWAY | Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. |
| PATHWAY | Porphyrin-containing compound metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. |
| PATHWAY | Porphyrin-containing compound metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. |
| PATHWAY | Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. |
| SIMILARITY | In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family. |
| SIMILARITY | In the C-terminal section; belongs to the precorrin methyltransferase family. |
Keywords
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| Cobalamin biosynthesis | |
| Lyase | |
| Methyltransferase | |
| Multifunctional enzyme | |
| NAD | |
| Oxidoreductase | |
| case <FT:13> | |
| Phosphoprotein | |
| end case | |
| Porphyrin biosynthesis | |
| S-adenosyl-L-methionine | |
| Transferase | |
Gene Ontology
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| GO:0004325; Molecular function:ferrochelatase activity |
| GO:0004851; Molecular function:uroporphyrin-III C-methyltransferase activity |
| GO:0043115; Molecular function:precorrin-2 dehydrogenase activity |
| GO:0006779; Biological process:porphyrin-containing compound biosynthetic process |
| GO:0009236; Biological process:cobalamin biosynthetic process |
| GO:0019354; Biological process:siroheme biosynthetic process |
Cross-references
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| PROSITE | PS00839; SUMT_1; 1; |
| PROSITE | PS00840; SUMT_2; 1; |
| Pfam | PF00590; TP_methylase; 1; |
| NCBIfam | TIGR01469; CobA_cysG_Cterm; 1; |
| NCBIfam | TIGR01470; CysG_Nterm; 1; |
Features
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| From: CYSG_SALTY (P25924) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 22 | 23 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
|||||||||
| BINDING | 43 | 44 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
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| REGION | Nter | 204 | /note="Precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase" | |||||||||
| REGION | 216 | Cter | /note="Uroporphyrinogen-III C-methyltransferase" | |||||||||
| BINDING | 301 | 303 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G-G-D | ||||||||
| BINDING | 331 | 332 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[TS]-A | ||||||||
| ACT_SITE | 248 | 248 | /note="Proton acceptor" | D | ||||||||
| ACT_SITE | 270 | 270 | /note="Proton donor" | K | ||||||||
| BINDING | 225 | 225 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
P | ||||||||
| BINDING | 306 | 306 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[IVL] | ||||||||
| BINDING | 382 | 382 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
M | ||||||||
| BINDING | 411 | 411 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[GA] | ||||||||
| MOD_RES | 128 | 128 | /note="Phosphoserine" | S | ||||||||
Additional information
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| Size range | 457-549 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |