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HAMAP rule MF_01656

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General rule information [?]

Accession MF_01656
Dates 30-APR-2008 (Created)
1-JUN-2023 (Last updated, Version 37)
Name HOA
Scope(s) Bacteria
Template(s) P51020 (HOA_ECOLI); P51015 (HOA4_PARXL); Q9KWS0 (HOA_PSESP); P51014 (HOA_PSES1); Q51983 (HOA2_PSEP1); P51016 (HOA_PSEUF); P51018 (HOA1_PSEP1); P51019 (HOA2_PSEPU); Q53WI0 (HOA_THET8); [ Recover all ]
Triggered by HAMAP; MF_01656 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier HOA
Protein name RecName: Full=4-hydroxy-2-oxovalerate aldolase;
                 Short=HOA;
                 EC=4.1.3.39;
AltName: Full=4-hydroxy-2-keto-pentanoic acid aldolase;
AltName: Full=4-hydroxy-2-oxopentanoate aldolase;
case <OC:Enterobacterales>
Gene name Name=mhpE;
end case

Comments [?]

case <OC:Enterobacterales>
FUNCTIONCatalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta- cleavage pathway for the degradation of aromatic compounds.
end case
CATALYTIC ACTIVITY Reaction=(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate; Xref=Rhea:RHEA:22624, ChEBI:CHEBI:15343, ChEBI:CHEBI:15361, ChEBI:CHEBI:73143; EC=4.1.3.39;
case <OC:Enterobacterales>
PATHWAYAromatic compound metabolism; 3-phenylpropanoate degradation.
SUBUNITInteracts with MhpF.
end case
SIMILARITYBelongs to the 4-hydroxy-2-oxovalerate aldolase family.

Keywords [?]


Gene Ontology [?]

GO:0008701; Molecular function:4-hydroxy-2-oxovalerate aldolase activity
GO:0030145; Molecular function:manganese ion binding
GO:0019439; Biological process:aromatic compound catabolic process

Cross-references [?]

PROSITE PS50991; PYR_CT; 1;
Pfam PF07836; DmpG_comm; 1;
Pfam PF00682; HMGL-like; 1;
NCBIfam TIGR03217; 4OH_2_O_val_ald; 1;

Features [?]

From: HOA_PSEUF (P51016)
Key From To Description Tag Condition FTGroup
BINDING 17 18 /ligand="substrate" R-D
ACT_SITE 21 21 /note="Proton acceptor" H
BINDING 18 18 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
D
BINDING 200 200 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
H
BINDING 202 202 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
H
BINDING 171 171 /ligand="substrate" S
BINDING 200 200 /ligand="substrate" H
BINDING 291 291 /ligand="substrate" Y
SITE 17 17 /note="Transition state stabilizer" R

Additional information [?]

Size range 333-377 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments This protein is present in multiple copies in some organisms, each one being located in a cluster involved in the degragation of a different aromatic compound. The E.coli protein showed no observable dependence on divalent metal ions (and no loss of enzyme activity by treatment with EDTA) unlike the purified Pseudomonas sp. strain CF600 and Burkholderia xenovorans aldolases.



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