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Annotation rule MF_01682 |
Accession | MF_01682 |
Dates | 4-NOV-2008 (Created) 19-NOV-2019 (Last updated, Version 17) |
Name | Salvage_MtnD |
Scope | Bacteria |
Templates | Q9ZFE7 (MTND_KLEOX); O31669 (MTND_BACSU): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. |
Catalytic activity | RHEA:14161: 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)
EC 1.13.11.53 |
RHEA:24504: 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)
EC 1.13.11.54 |
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Cofactor | Fe cation Note: Binds 1 Fe cation per monomer. |
Ni(2+) Note: Binds 1 nickel ion per monomer. |
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Pathway | Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6. |
Subunit | Monomer. |
Similarity | Belongs to the acireductone dioxygenase (ARD) family. |
Pfam | PF03079; ARD; 1; |
From: MTND_KLEOX (Q9ZFE7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
METAL | 97 | 97 | Iron; alternate | H | ||||||||
METAL | 97 | 97 | Nickel; alternate | H | ||||||||
METAL | 99 | 99 | Iron; alternate | H | ||||||||
METAL | 99 | 99 | Nickel; alternate | H | ||||||||
METAL | 103 | 103 | Iron; alternate | E | ||||||||
METAL | 103 | 103 | Nickel; alternate | E | ||||||||
METAL | 141 | 141 | Iron; alternate | H | ||||||||
METAL | 141 | 141 | Nickel; alternate | H | ||||||||
SITE (Optional) | 96 | 96 | May play a role in metal incorporation in vivo | E | ||||||||
SITE (Optional) | 102 | 102 | May play a role in transmitting local conformational changes | D | ||||||||
SITE (Optional) | 105 | 105 | Important to generate the dianion | R |
Size range | 165-206 amino acids |
Related rules | None |
Fusion | None |