HAMAP rule MF_01682

General rule information [?]

Accession	MF_01682
Dates	4-NOV-2008 (Created) 19-NOV-2022 (Last updated, Version 20)

Name	Salvage_MtnD

Scope

Bacteria

Templates

Q9ZFE7 (MTND_KLEOX); O31669 (MTND_BACSU): [Recover all]

Triggered by

HAMAP; MF_01682 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MTND

Protein name

RecName:		Full=Acireductone dioxygenase;
AltName:		Full=1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase;
		Short=DHK-MTPene dioxygenase;
AltName:		Full=Acireductone dioxygenase (Fe(2+)-requiring);
		Short=ARD';
		Short=Fe-ARD;
		EC 1.13.11.54;
AltName:		Full=Acireductone dioxygenase (Ni(2+)-requiring);
		Short=ARD;
		Short=Ni-ARD;
		EC 1.13.11.53;

Gene name

mtnD

Comments [?]

Function	Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
Catalytic activity	RHEA:14161: 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+) EC 1.13.11.53
	RHEA:24504: 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+) EC 1.13.11.54
Cofactor	Fe(2+) Note: Binds 1 Fe(2+) cation per monomer.
	Ni(2+) Note: Binds 1 nickel ion per monomer.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.
Subunit	Monomer.
Similarity	Belongs to the acireductone dioxygenase (ARD) family.

Keywords [?]

Amino-acid biosynthesis
Dioxygenase
Iron
Metal-binding
Methionine biosynthesis
Nickel
Oxidoreductase

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0010308; Molecular function: acireductone dioxygenase (Ni2+-requiring) activity.
GO:0010309; Molecular function: acireductone dioxygenase [iron(II)-requiring] activity.
GO:0016151; Molecular function: nickel cation binding.
GO:0019284; Biological process: L-methionine salvage from S-adenosylmethionine.

Cross-references [?]

Pfam	PF03079; ARD; 1;

Features [?]

From: MTND_KLEOX (Q9ZFE7)

Key From To Description Tag Condition FTGroup

BINDING 97 97 /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033 H

BINDING 97 97 /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786 H

BINDING 99 99 /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033 H

BINDING 99 99 /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786 H

BINDING 103 103 /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033 E

BINDING 103 103 /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786 E

BINDING 141 141 /ligand="Fe(2+)" /ligand_id="ChEBI:CHEBI:29033 H

BINDING 141 141 /ligand="Ni(2+)" /ligand_id="ChEBI:CHEBI:49786 H

SITE (Optional) 96 96 May play a role in metal incorporation in vivo E

SITE (Optional) 102 102 May play a role in transmitting local conformational changes D

SITE (Optional) 105 105 Important to generate the dianion R

Additional information [?]

Size range	165-206 amino acids
Related rules	None
Fusion	None

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