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Annotation rule MF_01682
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General rule information [?]

Accession MF_01682
Dates 4-NOV-2008 (Created)
19-NOV-2019 (Last updated, Version 17)
Name Salvage_MtnD
Templates Q9ZFE7 (MTND_KLEOX); O31669 (MTND_BACSU): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Acireductone dioxygenase;
AltName: Full=1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase;
Short=DHK-MTPene dioxygenase;
AltName: Full=Acireductone dioxygenase (Fe(2+)-requiring);
AltName: Full=Acireductone dioxygenase (Ni(2+)-requiring);
Gene name

Comments [?]

Function Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
Catalytic activity RHEA:14161: 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H(+)
RHEA:24504: 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 4-methylsulfanyl-2-oxobutanoate + formate + 2 H(+)
Cofactor Fe cation
Note: Binds 1 Fe cation per monomer.
Note: Binds 1 nickel ion per monomer.
Pathway Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.
Subunit Monomer.
Similarity Belongs to the acireductone dioxygenase (ARD) family.

Keywords [?]

Gene Ontology [?]

GO:0005506; Molecular function: iron ion binding.
GO:0010308; Molecular function: acireductone dioxygenase (Ni2+-requiring) activity.
GO:0010309; Molecular function: acireductone dioxygenase [iron(II)-requiring] activity.
GO:0016151; Molecular function: nickel cation binding.
GO:0019284; Biological process: L-methionine salvage from S-adenosylmethionine.

Cross-references [?]

Pfam PF03079; ARD; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
METAL     97     97       Iron; alternate     H  
METAL     97     97       Nickel; alternate     H  
METAL     99     99       Iron; alternate     H  
METAL     99     99       Nickel; alternate     H  
METAL     103     103       Iron; alternate     E  
METAL     103     103       Nickel; alternate     E  
METAL     141     141       Iron; alternate     H  
METAL     141     141       Nickel; alternate     H  
SITE (Optional)     96     96       May play a role in metal incorporation in vivo     E  
SITE (Optional)     102     102       May play a role in transmitting local conformational changes     D  
SITE (Optional)     105     105       Important to generate the dianion     R  

Additional information [?]

Size range 165-206 amino acids
Related rules None
Fusion None