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Annotation rule MF_01684 |
General rule information
[?]
Accession |
MF_01684 |
Dates |
19-NOV-2008 (Created) 25-MAY-2020 (Last updated, Version 20) |
Propagated annotation
[?]
Identifier, protein and gene names
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Protein name |
RecName:
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Full=5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase;
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Short=MTA/SAH nucleosidase;
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Short=MTAN;
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EC 3.2.2.9;
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AltName:
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Full=5'-deoxyadenosine nucleosidase;
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Short=DOA nucleosidase;
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Short=dAdo nucleosidase;
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AltName:
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Full=5'-methylthioadenosine nucleosidase;
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Short=MTA nucleosidase;
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AltName:
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Full=S-adenosylhomocysteine nucleosidase;
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Short=AdoHcy nucleosidase;
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Short=SAH nucleosidase;
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Short=SRH nucleosidase;
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case <OC:Enterobacterales>
Function |
Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation. |
else
Function |
Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. |
end case
Catalytic activity |
RHEA:17805: H2O + S-adenosyl-L-homocysteine = adenine + S-(5-deoxy-D-ribos-5-yl)-L-homocysteine
EC 3.2.2.9
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RHEA:13617: H2O + S-methyl-5'-thioadenosine = 5-(methylsulfanyl)-D-ribose + adenine
EC 3.2.2.9
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RHEA:29859: 5'-deoxyadenosine + H2O = 5-deoxy-D-ribose + adenine
EC 3.2.2.9
PhysiologicalDirection=left-to-right
(RHEA:29860)
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Pathway |
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (hydrolase route): step 1/2. |
case <OC:Enterobacterales>
end case
Similarity |
Belongs to the PNP/UDP phosphorylase family. MtnN subfamily. |
GO:0008782; Molecular function: adenosylhomocysteine nucleosidase activity.
GO:0008930; Molecular function: methylthioadenosine nucleosidase activity.
GO:0019284; Biological process: L-methionine salvage from S-adenosylmethionine.
case <OC:Enterobacterales>
GO:0046124; Biological process: purine deoxyribonucleoside catabolic process.
end case
From: MTNN_ECOLI (P0AF12) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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REGION
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173
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174
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Substrate binding
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M-[ED]
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ACT_SITE
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12
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12
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Proton acceptor
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E
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ACT_SITE
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197
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197
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Proton donor
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D
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BINDING
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78
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78
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Substrate; via amide nitrogen
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[GA]
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BINDING
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152
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152
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Substrate; via amide nitrogen and carbonyl oxygen
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[IVML]
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Additional information
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Size range |
221-251 amino acids |
Related rules |
None |
Fusion |
None |