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HAMAP rule MF_01684

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_01684
Accession MF_01684
Dates 19-NOV-2008 (Created)
03-SEP-2024 (Last updated, Version 26)
Name Salvage_MtnN
Scope(s) Bacteria
Template(s) P0AF12; O32028; Q9R4A1; [ Recover all ]
Triggered by HAMAP; MF_01684 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier MTNN
Protein name RecName: Full=5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase;
                 Short=MTA/SAH nucleosidase;
                 Short=MTAN;
                 EC=3.2.2.9;
AltName: Full=5'-deoxyadenosine nucleosidase;
                 Short=DOA nucleosidase;
                 Short=dAdo nucleosidase;
AltName: Full=5'-methylthioadenosine nucleosidase;
                 Short=MTA nucleosidase;
AltName: Full=S-adenosylhomocysteine nucleosidase;
                 Short=AdoHcy nucleosidase;
                 Short=SAH nucleosidase;
                 Short=SRH nucleosidase;
Gene name Name=mtnN;

Comments [?]

case <OC:Enterobacterales>
FUNCTIONCatalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'- methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine. Thus, is required for in vivo function of the radical SAM enzymes biotin synthase and lipoic acid synthase, that are inhibited by 5'-deoxyadenosine accumulation.
else
FUNCTIONCatalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'- methylthioribose and S-ribosylhomocysteine, respectively. Also cleaves 5'-deoxyadenosine, a toxic by-product of radical S-adenosylmethionine (SAM) enzymes, into 5-deoxyribose and adenine.
end case
CATALYTIC ACTIVITY Reaction=S-adenosyl-L-homocysteine + H2O = S-(5-deoxy-D-ribos-5-yl)-L- homocysteine + adenine; Xref=Rhea:RHEA:17805, ChEBI:CHEBI:15377, ChEBI:CHEBI:16708, ChEBI:CHEBI:57856, ChEBI:CHEBI:58195; EC=3.2.2.9;
CATALYTIC ACTIVITY Reaction=S-methyl-5'-thioadenosine + H2O = 5-(methylsulfanyl)-D-ribose + adenine; Xref=Rhea:RHEA:13617, ChEBI:CHEBI:15377, ChEBI:CHEBI:16708, ChEBI:CHEBI:17509, ChEBI:CHEBI:78440; EC=3.2.2.9;
CATALYTIC ACTIVITY Reaction=5'-deoxyadenosine + H2O = 5-deoxy-D-ribose + adenine; Xref=Rhea:RHEA:29859, ChEBI:CHEBI:15377, ChEBI:CHEBI:16708, ChEBI:CHEBI:17319, ChEBI:CHEBI:149540; EC=3.2.2.9; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:29860;
PATHWAYAmino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'- thioadenosine (hydrolase route): step 1/2.
case <OC:Enterobacterales>
SUBUNITHomodimer.
end case
SIMILARITYBelongs to the PNP/UDP phosphorylase family. MtnN subfamily.

Keywords [?]

Amino-acid biosynthesis
Hydrolase
Methionine biosynthesis

Gene Ontology [?]

GO:0008782; Molecular function:adenosylhomocysteine nucleosidase activity
GO:0008930; Molecular function:methylthioadenosine nucleosidase activity
GO:0019509; Biological process:L-methionine salvage from methylthioadenosine
case <OCellular component:Enterobacterales>
GO:0046124; Biological process:purine deoxyribonucleoside catabolic process
end case

Cross-references [?]

Pfam PF01048; PNP_UDP_1; 1;
NCBIfam TIGR01704; MTA/SAH-Nsdase; 1;

Features [?]

From: MTNN_ECOLI (P0AF12)
Key From To Description Tag Condition FTGroup
BINDING 173 174 /ligand="substrate" M-[ED]
ACT_SITE 12 12 /note="Proton acceptor" E
ACT_SITE 197 197 /note="Proton donor" D
BINDING 78 78 /ligand="substrate" [GA]
BINDING 152 152 /ligand="substrate" [IVML]

Additional information [?]

Size range 221-251 amino acids
Related rules None
Fusion Nter: None Cter: None



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