HAMAP rule MF_01687
General rule information
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| Accession | MF_01687 |
| Dates | 19-FEB-2009 (Created)
19-NOV-2022 (Last updated, Version 13) |
| Name | Beta_gal |
| Scope(s) |
Bacteria |
| Template(s) | P00722 (BGAL_ECOLI); A3FEW8 (BGAL_ENTAG); P81650 (BGAL_PSEHA); [ Recover all ] |
| Triggered by |
HAMAP; MF_01687 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | BGAL |
| Protein name | RecName: Full=Beta-galactosidase; Short=Beta-gal; EC=3.2.1.23; AltName: Full=Lactase; |
| Gene name | Name=lacZ; |
Comments
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| CATALYTIC ACTIVITY | Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.; EC=3.2.1.23; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per monomer.; |
| COFACTOR | Name=Na(+); Xref=ChEBI:CHEBI:29101; Note=Binds 1 sodium ion per monomer.; |
| SUBUNIT | Homotetramer. |
| SIMILARITY | Belongs to the glycosyl hydrolase 2 family. |
Keywords
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Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0016798; Molecular function:hydrolase activity, acting on glycosyl bonds |
Cross-references
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| PROSITE | PS00719; GLYCOSYL_HYDROL_F2_1; 1; |
| PROSITE | PS00608; GLYCOSYL_HYDROL_F2_2; 1; |
| Pfam | PF02929; Bgal_small_N; 1; |
| Pfam | PF00703; Glyco_hydro_2; 1; |
| Pfam | PF02836; Glyco_hydro_2_C; 1; |
| Pfam | PF02837; Glyco_hydro_2_N; 1; |
| PRINTS | PR00132; GLHYDRLASE2; 1; |
Features
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| From: BGAL_ECOLI (P00722) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 538 | 541 | /ligand="substrate" | E-Y-A-H | ||||||||
| ACT_SITE | 462 | 462 | /note="Proton donor" | E | ||||||||
| ACT_SITE | 538 | 538 | /note="Nucleophile" | E | ||||||||
| BINDING | 202 | 202 | /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101" |
D | ||||||||
| BINDING | 417 | 417 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 419 | 419 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
H | ||||||||
| BINDING | 462 | 462 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1" |
E | ||||||||
| BINDING | 598 | 598 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2" |
N | ||||||||
| BINDING | 602 | 602 | /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101" |
F | ||||||||
| BINDING | 605 | 605 | /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101" |
[DN] | ||||||||
| BINDING | 103 | 103 | /ligand="substrate" | N | ||||||||
| BINDING | 202 | 202 | /ligand="substrate" | D | ||||||||
| BINDING | 462 | 462 | /ligand="substrate" | E | ||||||||
| BINDING | 605 | 605 | /ligand="substrate" | [DN] | ||||||||
| BINDING | 1000 | 1000 | /ligand="substrate" | W | ||||||||
| SITE | 358 | 358 | /note="Transition state stabilizer" | H | ||||||||
| SITE | 392 | 392 | /note="Transition state stabilizer" | H | ||||||||
Additional information
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| Size range | 1020-1066 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | ENTAG, and maybe other bacteria, are able to transfer a glycosyl to the hydroxyl groups of a series of accecptors including hexose, pentose, disaccharides, hexahydroxy alcohol, cyclitol, and aromatic glycosides. |