HAMAP rule MF_01687

General rule information [?]

Accession	MF_01687
Dates	19-FEB-2009 (Created) 19-NOV-2022 (Last updated, Version 13)

Name

Beta_gal

Scope

Bacteria

Templates

P00722 (BGAL_ECOLI); A3FEW8 (BGAL_ENTAG); P81650 (BGAL_PSEHA): [Recover all]

Triggered by

HAMAP; MF_01687 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

BGAL

Protein name

RecName:		Full=Beta-galactosidase;
		Short=Beta-gal;
		EC 3.2.1.23;
AltName:		Full=Lactase;

Gene name

lacZ

Comments [?]

Catalytic activity	Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.; EC=3.2.1.23;
Cofactor	Mg(2+) Note: Binds 2 magnesium ions per monomer.
	Na(+) Note: Binds 1 sodium ion per monomer.
Subunit	Homotetramer.
Similarity	Belongs to the glycosyl hydrolase 2 family.

Keywords [?]

Glycosidase
Hydrolase
Magnesium
Metal-binding
Sodium

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0016798; Molecular function: hydrolase activity, acting on glycosyl bonds.

Cross-references [?]

PROSITE	PS00719; GLYCOSYL_HYDROL_F2_1; 1;
	PS00608; GLYCOSYL_HYDROL_F2_2; 1;
Pfam	PF02929; Bgal_small_N; 1;
	PF00703; Glyco_hydro_2; 1;
	PF02836; Glyco_hydro_2_C; 1;
	PF02837; Glyco_hydro_2_N; 1;
PRINTS	PR00132; GLHYDRLASE2; 1;

Features [?]

From: BGAL_ECOLI (P00722)

Key From To Description Tag Condition FTGroup

BINDING 538 541 /ligand="substrate E-Y-A-H

ACT_SITE 462 462 Proton donor E

ACT_SITE 538 538 Nucleophile E

BINDING 202 202 /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101 D

BINDING 417 417 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 E

BINDING 419 419 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 H

BINDING 462 462 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 E

BINDING 598 598 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 N

BINDING 602 602 /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101 F

BINDING 605 605 /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101 [DN]

BINDING 103 103 /ligand="substrate N

BINDING 202 202 /ligand="substrate D

BINDING 462 462 /ligand="substrate E

BINDING 605 605 /ligand="substrate [DN]

BINDING 1000 1000 /ligand="substrate W

SITE 358 358 Transition state stabilizer H

SITE 392 392 Transition state stabilizer H

Additional information [?]

Size range	1020-1066 amino acids
Related rules	None
Fusion	None
Comments	ENTAG, and maybe other bacteria, are able to transfer a glycosyl to the hydroxyl groups of a series of accecptors including hexose, pentose, disaccharides, hexahydroxy alcohol, cyclitol, and aromatic glycosides.

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