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| Annotation rule MF_01687 |
General rule information
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| Accession | MF_01687 |
| Dates | 19-FEB-2009 (Created) 19-NOV-2019 (Last updated, Version 10) |
| Name | Beta_gal |
| Scope | Bacteria |
| Templates | P00722 (BGAL_ECOLI); A3FEW8 (BGAL_ENTAG); P81650 (BGAL_PSEHA): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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| Catalytic activity | Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.; EC=3.2.1.23; |
| Cofactor | Mg(2+) Note: Binds 2 magnesium ions per monomer. |
| Na(+) Note: Binds 1 sodium ion per monomer. |
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| Subunit | Homotetramer. |
| Similarity | Belongs to the glycosyl hydrolase 2 family. |
Keywords
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Gene Ontology
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GO:0000287; Molecular function: magnesium ion binding.
GO:0016798; Molecular function: hydrolase activity, acting on glycosyl bonds.
GO:0016798; Molecular function: hydrolase activity, acting on glycosyl bonds.
Cross-references
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| PROSITE | PS00719; GLYCOSYL_HYDROL_F2_1; 1; |
| PS00608; GLYCOSYL_HYDROL_F2_2; 1; | |
| Pfam | PF02929; Bgal_small_N; 1; |
| PF00703; Glyco_hydro_2; 1; | |
| PF02836; Glyco_hydro_2_C; 1; | |
| PF02837; Glyco_hydro_2_N; 1; | |
| PRINTS | PR00132; GLHYDRLASE2; 1; |
Features
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| From: BGAL_ECOLI (P00722) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 538 | 541 | Substrate binding | E-Y-A-H | ||||||||
| ACT_SITE | 462 | 462 | Proton donor | E | ||||||||
| ACT_SITE | 538 | 538 | Nucleophile | E | ||||||||
| METAL | 202 | 202 | Sodium | D | ||||||||
| METAL | 417 | 417 | Magnesium 1 | E | ||||||||
| METAL | 419 | 419 | Magnesium 1 | H | ||||||||
| METAL | 462 | 462 | Magnesium 1 | E | ||||||||
| METAL | 598 | 598 | Magnesium 2 | N | ||||||||
| METAL | 602 | 602 | Sodium; via carbonyl oxygen | F | ||||||||
| METAL | 605 | 605 | Sodium | [DN] | ||||||||
| BINDING | 103 | 103 | Substrate | N | ||||||||
| BINDING | 202 | 202 | Substrate | D | ||||||||
| BINDING | 462 | 462 | Substrate | E | ||||||||
| BINDING | 605 | 605 | Substrate | [DN] | ||||||||
| BINDING | 1000 | 1000 | Substrate | W | ||||||||
| SITE | 358 | 358 | Transition state stabilizer | H | ||||||||
| SITE | 392 | 392 | Transition state stabilizer | H | ||||||||
Additional information
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| Size range | 1020-1066 amino acids |
| Related rules | None |
| Fusion | None |
| Comments | ENTAG, and maybe other bacteria, are able to transfer a glycosyl to the hydroxyl groups of a series of accecptors including hexose, pentose, disaccharides, hexahydroxy alcohol, cyclitol, and aromatic glycosides. |