HAMAP rule MF_01687
General rule information
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Accession | MF_01687 |
Dates | 19-FEB-2009 (Created) 19-NOV-2022 (Last updated, Version 13) |
Name | Beta_gal |
Scope | Bacteria |
Templates | P00722 (BGAL_ECOLI); A3FEW8 (BGAL_ENTAG); P81650 (BGAL_PSEHA): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Catalytic activity | Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.; EC=3.2.1.23; |
Cofactor | Mg(2+) Note: Binds 2 magnesium ions per monomer. |
Na(+) Note: Binds 1 sodium ion per monomer. |
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Subunit | Homotetramer. |
Similarity | Belongs to the glycosyl hydrolase 2 family. |
Keywords
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Gene Ontology
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GO:0000287; Molecular function: magnesium ion binding.
GO:0016798; Molecular function: hydrolase activity, acting on glycosyl bonds.
GO:0016798; Molecular function: hydrolase activity, acting on glycosyl bonds.
Cross-references
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PROSITE | PS00719; GLYCOSYL_HYDROL_F2_1; 1; |
PS00608; GLYCOSYL_HYDROL_F2_2; 1; | |
Pfam | PF02929; Bgal_small_N; 1; |
PF00703; Glyco_hydro_2; 1; | |
PF02836; Glyco_hydro_2_C; 1; | |
PF02837; Glyco_hydro_2_N; 1; | |
PRINTS | PR00132; GLHYDRLASE2; 1; |
Features
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From: BGAL_ECOLI (P00722) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 538 | 541 | /ligand="substrate | E-Y-A-H | ||||||||
ACT_SITE | 462 | 462 | Proton donor | E | ||||||||
ACT_SITE | 538 | 538 | Nucleophile | E | ||||||||
BINDING | 202 | 202 | /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101 | D | ||||||||
BINDING | 417 | 417 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 | E | ||||||||
BINDING | 419 | 419 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 | H | ||||||||
BINDING | 462 | 462 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="1 | E | ||||||||
BINDING | 598 | 598 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_label="2 | N | ||||||||
BINDING | 602 | 602 | /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101 | F | ||||||||
BINDING | 605 | 605 | /ligand="Na(+)" /ligand_id="ChEBI:CHEBI:29101 | [DN] | ||||||||
BINDING | 103 | 103 | /ligand="substrate | N | ||||||||
BINDING | 202 | 202 | /ligand="substrate | D | ||||||||
BINDING | 462 | 462 | /ligand="substrate | E | ||||||||
BINDING | 605 | 605 | /ligand="substrate | [DN] | ||||||||
BINDING | 1000 | 1000 | /ligand="substrate | W | ||||||||
SITE | 358 | 358 | Transition state stabilizer | H | ||||||||
SITE | 392 | 392 | Transition state stabilizer | H |
Additional information
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Size range | 1020-1066 amino acids |
Related rules | None |
Fusion | None |
Comments | ENTAG, and maybe other bacteria, are able to transfer a glycosyl to the hydroxyl groups of a series of accecptors including hexose, pentose, disaccharides, hexahydroxy alcohol, cyclitol, and aromatic glycosides. |