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Annotation rule MF_01687
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General rule information [?]

Accession MF_01687
Dates 19-FEB-2009 (Created)
19-NOV-2019 (Last updated, Version 10)
Name Beta_gal
Scope
Bacteria
Templates P00722 (BGAL_ECOLI); A3FEW8 (BGAL_ENTAG); P81650 (BGAL_PSEHA): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
BGAL
Protein name
RecName: Full=Beta-galactosidase;
Short=Beta-gal;
EC 3.2.1.23;
AltName: Full=Lactase;
Gene name
lacZ

Comments [?]

Catalytic activity Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.; EC=3.2.1.23;
Cofactor Mg(2+)
Note: Binds 2 magnesium ions per monomer.
Na(+)
Note: Binds 1 sodium ion per monomer.
Subunit Homotetramer.
Similarity Belongs to the glycosyl hydrolase 2 family.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0016798; Molecular function: hydrolase activity, acting on glycosyl bonds.

Cross-references [?]

PROSITE PS00719; GLYCOSYL_HYDROL_F2_1; 1;
PS00608; GLYCOSYL_HYDROL_F2_2; 1;
Pfam PF02929; Bgal_small_N; 1;
PF00703; Glyco_hydro_2; 1;
PF02836; Glyco_hydro_2_C; 1;
PF02837; Glyco_hydro_2_N; 1;
PRINTS PR00132; GLHYDRLASE2; 1;

Features [?]

From: BGAL_ECOLI (P00722)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     538     541       Substrate binding     E-Y-A-H  
ACT_SITE     462     462       Proton donor     E  
ACT_SITE     538     538       Nucleophile     E  
METAL     202     202       Sodium     D  
METAL     417     417       Magnesium 1     E  
METAL     419     419       Magnesium 1     H  
METAL     462     462       Magnesium 1     E  
METAL     598     598       Magnesium 2     N  
METAL     602     602       Sodium; via carbonyl oxygen     F  
METAL     605     605       Sodium     [DN]  
BINDING     103     103       Substrate     N  
BINDING     202     202       Substrate     D  
BINDING     462     462       Substrate     E  
BINDING     605     605       Substrate     [DN]  
BINDING     1000     1000       Substrate     W  
SITE     358     358       Transition state stabilizer     H  
SITE     392     392       Transition state stabilizer     H  

Additional information [?]

Size range 1020-1066 amino acids
Related rules None
Fusion None
Comments ENTAG, and maybe other bacteria, are able to transfer a glycosyl to the hydroxyl groups of a series of accecptors including hexose, pentose, disaccharides, hexahydroxy alcohol, cyclitol, and aromatic glycosides.