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Annotation rule MF_01693 |
Accession | MF_01693 |
Dates | 1-JUL-2009 (Created) 17-APR-2020 (Last updated, Version 22) |
Name | BioF_aminotrans_2 |
Scope | Bacteria; Proteobacteria |
Template | P12998 (BIOF_ECOLI) |
Triggered by |
Identifier |
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Protein name |
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Gene name |
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Function | Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide. |
Catalytic activity | RHEA:42288: 6-carboxyhexanoyl-[ACP] + H(+) + L-alanine = (S)-8-amino-7-oxononanoate + CO2 + holo-[ACP]
EC 2.3.1.47 |
Cofactor | pyridoxal 5'-phosphate |
Pathway | Cofactor biosynthesis; biotin biosynthesis. |
Subunit | Homodimer. |
Similarity | Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily. |
PROSITE | PS00599; AA_TRANSFER_CLASS_2; 1; |
Pfam | PF00155; Aminotran_1_2; 1; |
TIGRFAMs | TIGR00858; BioF; 1; |
From: BIOF_ECOLI (P12998) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 108 | 109 | Pyridoxal phosphate binding | G-[FY] | ||||||||
BINDING | 21 | 21 | Substrate | R | ||||||||
BINDING | 133 | 133 | Substrate | H | ||||||||
BINDING | 179 | 179 | Pyridoxal phosphate | S | ||||||||
BINDING | 207 | 207 | Pyridoxal phosphate | H | ||||||||
BINDING | 233 | 233 | Pyridoxal phosphate | T | ||||||||
BINDING | 352 | 352 | Substrate | T | ||||||||
MOD_RES | 236 | 236 | N6-(pyridoxal phosphate)lysine | K |
Size range | 377-471 amino acids |
Related rules | None |
Fusion | Nter: MF_00668 (bioW); Cter: None |
Comments | In most cases, AON synthase activity has been tested with pimeloyl-CoA as substrate, however it is believed that pimeloyl-ACP rather than pimeloyl-CoA is the physiological substrate of BioF (PubMed:20693992). BioF (8-amino-7-oxononanoate synthase)(TIGR00858) and Kbl (2-amino-3-ketobutyrate coenzyme A ligase)(TIGR01822) catalyze very close reactions, and alignment of the amino acid sequences of both enzymes reveals a very close homology, which does not allow to separate them. The only way to create a specific family for the BioF enzymes is to limit the rule to Proteobacteria. A few Firmicutes (LYSSH and some close orthologs), are annotated as a 8-amino-7-oxononanoate synthase (BioF) due to their characterisation but are not members of the family. The rest of proteins which cannot be differentiated, is annotated as a putative 8-amino-7-oxononanoate synthase in DE line and are not members of the family. Proteins with both activites (TIGR00858/TIGR01825) are annotated as putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase in DE lines and BIKB in ID lines. |