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Annotation rule MF_01693
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General rule information [?]

Accession MF_01693
Dates 1-JUL-2009 (Created)
17-APR-2020 (Last updated, Version 22)
Name BioF_aminotrans_2
Scope
Bacteria; Proteobacteria
Template P12998 (BIOF_ECOLI)
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
BIOF
Protein name
RecName: Full=8-amino-7-oxononanoate synthase;
Short=AONS;
EC 2.3.1.47;
AltName: Full=7-keto-8-amino-pelargonic acid synthase;
Short=7-KAP synthase;
Short=KAPA synthase;
AltName: Full=8-amino-7-ketopelargonate synthase;
Gene name
bioF

Comments [?]

Function Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.
Catalytic activity RHEA:42288: 6-carboxyhexanoyl-[ACP] + H(+) + L-alanine = (S)-8-amino-7-oxononanoate + CO2 + holo-[ACP]
EC 2.3.1.47
Cofactor pyridoxal 5'-phosphate
Pathway Cofactor biosynthesis; biotin biosynthesis.
Subunit Homodimer.
Similarity Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.

Keywords [?]

Biotin biosynthesis
Pyridoxal phosphate
Transferase

Gene Ontology [?]

GO:0008710; Molecular function: 8-amino-7-oxononanoate synthase activity.
GO:0030170; Molecular function: pyridoxal phosphate binding.
GO:0009102; Biological process: biotin biosynthetic process.

Cross-references [?]

PROSITE PS00599; AA_TRANSFER_CLASS_2; 1;
Pfam PF00155; Aminotran_1_2; 1;
TIGRFAMs TIGR00858; BioF; 1;

Features [?]

From: BIOF_ECOLI (P12998)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     108     109       Pyridoxal phosphate binding     G-[FY]  
BINDING     21     21       Substrate     R  
BINDING     133     133       Substrate     H  
BINDING     179     179       Pyridoxal phosphate     S  
BINDING     207     207       Pyridoxal phosphate     H  
BINDING     233     233       Pyridoxal phosphate     T  
BINDING     352     352       Substrate     T  
MOD_RES     236     236       N6-(pyridoxal phosphate)lysine     K  

Additional information [?]

Size range 377-471 amino acids
Related rules None
Fusion Nter: MF_00668 (bioW); Cter: None
Comments In most cases, AON synthase activity has been tested with pimeloyl-CoA as substrate, however it is believed that pimeloyl-ACP rather than pimeloyl-CoA is the physiological substrate of BioF (PubMed:20693992). BioF (8-amino-7-oxononanoate synthase)(TIGR00858) and Kbl (2-amino-3-ketobutyrate coenzyme A ligase)(TIGR01822) catalyze very close reactions, and alignment of the amino acid sequences of both enzymes reveals a very close homology, which does not allow to separate them. The only way to create a specific family for the BioF enzymes is to limit the rule to Proteobacteria. A few Firmicutes (LYSSH and some close orthologs), are annotated as a 8-amino-7-oxononanoate synthase (BioF) due to their characterisation but are not members of the family. The rest of proteins which cannot be differentiated, is annotated as a putative 8-amino-7-oxononanoate synthase in DE line and are not members of the family. Proteins with both activites (TIGR00858/TIGR01825) are annotated as putative 8-amino-7-oxononanoate synthase/2-amino-3-ketobutyrate coenzyme A ligase in DE lines and BIKB in ID lines.


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