Home  |  Contact
Annotation rule MF_01694
Send feedback

General rule information [?]

Accession MF_01694
Dates 2-JUL-2009 (Created)
17-APR-2020 (Last updated, Version 16)
Name BioB
Template P12996 (BIOB_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Biotin synthase;
Gene name

Comments [?]

Function Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.
Catalytic activity RHEA:22060: (4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
[2Fe-2S] cluster
Note: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.
Pathway Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.
Subunit Homodimer.
Similarity Belongs to the radical SAM superfamily. Biotin synthase family.

Keywords [?]

Gene Ontology [?]

GO:0004076; Molecular function: biotin synthase activity.
GO:0005506; Molecular function: iron ion binding.
GO:0051536; Molecular function: iron-sulfur cluster binding.
GO:0009102; Biological process: biotin biosynthetic process.

Cross-references [?]

Pfam PF06968; BATS; 1;
PF04055; Radical_SAM; 1;
TIGRFAMs TIGR00433; BioB; 1;

Features [?]

From: BIOB_ECOLI (P12996)
Key     From     To       Description   Tag   Condition   FTGroup
METAL     53     53       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     57     57       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     60     60       Iron-sulfur 1 (4Fe-4S-S-AdoMet)     C  
METAL     97     97       Iron-sulfur 2 (2Fe-2S)     [CS]  
METAL     128     128       Iron-sulfur 2 (2Fe-2S)     [CS]  
METAL     188     188       Iron-sulfur 2 (2Fe-2S)     C  
METAL     260     260       Iron-sulfur 2 (2Fe-2S)     [KR]  

Additional information [?]

Size range 275-804 amino acids
Related rules None
Fusion Nter: None; Cter: MF_00834 (bioA)
Comments Bacteroides encode a fusion between BioA (7,8-diamino-pelargonic acid aminotransferase) and BioB.