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HAMAP rule MF_01694

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General rule information [?]

Accession MF_01694
Dates 2-JUL-2009 (Created)
1-JUN-2023 (Last updated, Version 21)
Name BioB
Scope(s) Bacteria
Template(s) P12996 (BIOB_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01694 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier BIOB
Protein name RecName: Full=Biotin synthase;
Gene name Name=bioB;

Comments [?]

FUNCTIONCatalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.
CATALYTIC ACTIVITY Reaction=(4R,5S)-dethiobiotin + [sulfur carrier]-SH + 2 reduced [2Fe- 2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = 2 5'-deoxyadenosine + [sulfur carrier]-H + biotin + 2 L-methionine + 2 oxidized [2Fe-2S]- [ferredoxin]; Xref=Rhea:RHEA:22060, Rhea:RHEA-COMP:10000, Rhea:RHEA- COMP:10001, Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739, ChEBI:CHEBI:17319, ChEBI:CHEBI:29917, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:57586, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:149473; EC=;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.;
COFACTOR Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135; Note=Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.;
PATHWAYCofactor biosynthesis; biotin biosynthesis; biotin from 7,8- diaminononanoate: step 2/2.
SIMILARITYBelongs to the radical SAM superfamily. Biotin synthase family.

Keywords [?]

Gene Ontology [?]

GO:0004076; Molecular function:biotin synthase activity
GO:0005506; Molecular function:iron ion binding
GO:0051536; Molecular function:iron-sulfur cluster binding
GO:0009102; Biological process:biotin biosynthetic process

Cross-references [?]

Pfam PF06968; BATS; 1;
Pfam PF04055; Radical_SAM; 1;
NCBIfam TIGR00433; BioB; 1;

Features [?]

From: BIOB_ECOLI (P12996)
Key From To Description Tag Condition FTGroup
BINDING 53 53 /ligand="[4Fe-4S] cluster"
BINDING 57 57 /ligand="[4Fe-4S] cluster"
BINDING 60 60 /ligand="[4Fe-4S] cluster"
BINDING 97 97 /ligand="[2Fe-2S] cluster"
BINDING 128 128 /ligand="[2Fe-2S] cluster"
BINDING 188 188 /ligand="[2Fe-2S] cluster"
BINDING 260 260 /ligand="[2Fe-2S] cluster"

Additional information [?]

Size range 275-804 amino acids
Related rules None
Fusion Nter: None Cter: MF_00834 (bioA)
Comments Bacteroides encode a fusion between BioA (7,8-diamino-pelargonic acid aminotransferase) and BioB.

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