HAMAP rule MF_01695
General rule information
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| Accession | MF_01695 |
| Dates | 20-SEP-2010 (Created)
1-JUN-2023 (Last updated, Version 13) |
| Name | MshA |
| Scope(s) |
Bacteria Actinomycetota |
| Template(s) | Q8NTA6 (MSHA_CORGL); P9WMY7 (MSHA_MYCTU); A0QQZ8 (MSHA_MYCS2); [ Recover all ] |
| Triggered by |
HAMAP; MF_01695 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MSHA |
| Protein name | RecName: Full=D-inositol-3-phosphate glycosyltransferase; EC=2.4.1.250; AltName: Full=N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferase; Short=GlcNAc-Ins-P N-acetylglucosaminyltransferase; |
| Gene name | Name=mshA; |
Comments
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| FUNCTION | Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2- deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway. |
| CATALYTIC ACTIVITY | Reaction=1D-myo-inositol 3-phosphate + UDP-N-acetyl-alpha-D-glucosamine = 1D-myo-inositol 2-acetamido-2-deoxy-alpha-D-glucopyranoside 3- phosphate + H(+) + UDP; Xref=Rhea:RHEA:26188, ChEBI:CHEBI:15378, ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:58401, ChEBI:CHEBI:58892; EC=2.4.1.250; |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the glycosyltransferase group 1 family. MshA subfamily. |
Keywords
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Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0008375; Molecular function:acetylglucosaminyltransferase activity |
| GO:0010125; Biological process:mycothiol biosynthetic process |
Cross-references
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| Pfam | PF08323; Glyco_transf_5; 1; |
| Pfam | PF00534; Glycos_transf_1; 1; |
| NCBIfam | TIGR03449; Mycothiol_MshA; 1; |
Features
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| From: MSHA_CORGL (Q8NTA6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 15 | 16 | /ligand="UDP-N-acetyl-alpha-D-glucosamine" /ligand_id="ChEBI:CHEBI:57705" |
Q-P | ||||||||
| BINDING | 20 | 25 | /ligand="1D-myo-inositol 3-phosphate" /ligand_id="ChEBI:CHEBI:58401" |
D-x-G-x-[ML]-N | ||||||||
| BINDING | 303 | 303 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[YFIM] | ||||||||
| BINDING | 304 | 304 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[RHQ] | ||||||||
| BINDING | 306 | 306 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[ACSV] | ||||||||
| BINDING | 330 | 330 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
T | ||||||||
| BINDING | 9 | 9 | /ligand="1D-myo-inositol 3-phosphate" /ligand_id="ChEBI:CHEBI:58401" |
H | ||||||||
| BINDING | 23 | 23 | /ligand="UDP-N-acetyl-alpha-D-glucosamine" /ligand_id="ChEBI:CHEBI:57705" |
G | ||||||||
| BINDING | 78 | 78 | /ligand="1D-myo-inositol 3-phosphate" /ligand_id="ChEBI:CHEBI:58401" |
[KR] | ||||||||
| BINDING | 110 | 110 | /ligand="1D-myo-inositol 3-phosphate" /ligand_id="ChEBI:CHEBI:58401" |
[YH] | ||||||||
| BINDING | 134 | 134 | /ligand="1D-myo-inositol 3-phosphate" /ligand_id="ChEBI:CHEBI:58401" |
[TS] | ||||||||
| BINDING | 154 | 154 | /ligand="1D-myo-inositol 3-phosphate" /ligand_id="ChEBI:CHEBI:58401" |
[RQ] | ||||||||
| BINDING | 231 | 231 | /ligand="UDP-N-acetyl-alpha-D-glucosamine" /ligand_id="ChEBI:CHEBI:57705" |
R | ||||||||
| BINDING | 236 | 236 | /ligand="UDP-N-acetyl-alpha-D-glucosamine" /ligand_id="ChEBI:CHEBI:57705" |
K | ||||||||
| BINDING | 294 | 294 | /ligand="UDP-N-acetyl-alpha-D-glucosamine" /ligand_id="ChEBI:CHEBI:57705" |
[RMQV] | ||||||||
| BINDING | 316 | 316 | /ligand="UDP-N-acetyl-alpha-D-glucosamine" /ligand_id="ChEBI:CHEBI:57705" |
E | ||||||||
| BINDING | 324 | 324 | /ligand="UDP-N-acetyl-alpha-D-glucosamine" /ligand_id="ChEBI:CHEBI:57705" |
E | ||||||||
Additional information
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| Size range | 407-496 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |