HAMAP rule MF_01699
General rule information
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Accession | MF_01699 |
Dates | 2-NOV-2010 (Created) 1-JUN-2023 (Last updated, Version 15) |
Name | RutA |
Scope | Bacteria |
Template | P75898 (RUTA_ECOLI) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen atoms in the process to yield ureidoacrylate peracid, that immediately reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN-N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the flavin reductase RutF to regenerate FMN in vivo. |
Catalytic activity | RHEA:31587: FMNH2 + NADH + O2 + uracil = (Z)-3-ureidoacrylate + FMN + H(+) + H2O + NAD(+)
EC 1.14.99.46 |
RHEA:31599: FMNH2 + NADH + O2 + thymine = (Z)-2-methylureidoacrylate + FMN + H(+) + H2O + NAD(+)
EC 1.14.99.46 |
case <OC:Escherichia> or <OC:Shigella>
Induction | Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR. |
end case
Similarity | Belongs to the NtaA/SnaA/DszA monooxygenase family. RutA subfamily. |
Keywords
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Gene Ontology
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GO:0004497; Molecular function: monooxygenase activity.
GO:0006212; Biological process: uracil catabolic process.
GO:0019740; Biological process: nitrogen utilization.
GO:0006212; Biological process: uracil catabolic process.
GO:0019740; Biological process: nitrogen utilization.
Cross-references
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Features
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From: RUTA_ECOLI (P75898) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 68 | 69 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | I-K | ||||||||
BINDING | 159 | 160 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | R-Y | ||||||||
BINDING | 134 | 134 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | N | ||||||||
BINDING | 143 | 143 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | E | ||||||||
BINDING | 209 | 209 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 | S |
Additional information
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Size range | 331-393 amino acids |
Related rules | None |
Fusion | None |