HAMAP rule MF_01699

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01699
Accession	MF_01699
Dates	2-NOV-2010 (Created) 3-SEP-2024 (Last updated, Version 17)
Name	RutA
Scope(s)	Bacteria
Template(s)	P75898 (RUTA_ECOLI); [ Recover all ]
Triggered by	HAMAP; MF_01699 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	RUTA
Protein name	RecName: Full=Pyrimidine monooxygenase RutA; EC=1.14.99.46;
Gene name	Name=rutA;

Comments [?]

FUNCTION	Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen atoms in the process to yield ureidoacrylate peracid, that immediately reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN- N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the flavin reductase RutF to regenerate FMN in vivo.
CATALYTIC ACTIVITY	Reaction=uracil + FMNH2 + NADH + O2 = (Z)-3-ureidoacrylate + FMN + NAD(+) + H2O + H(+); Xref=Rhea:RHEA:31587, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17568, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618, ChEBI:CHEBI:57945, ChEBI:CHEBI:58210, ChEBI:CHEBI:59891; EC=1.14.99.46;
CATALYTIC ACTIVITY	Reaction=thymine + FMNH2 + NADH + O2 = (Z)-2-methylureidoacrylate + FMN + NAD(+) + H2O + H(+); Xref=Rhea:RHEA:31599, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17821, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618, ChEBI:CHEBI:57945, ChEBI:CHEBI:58210, ChEBI:CHEBI:143783; EC=1.14.99.46;
case <OC:Escherichia> or <OC:Shigella>
INDUCTION	Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.
end case
SIMILARITY	Belongs to the NtaA/SnaA/DszA monooxygenase family. RutA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004497; Molecular function:monooxygenase activity

GO:0006212; Biological process:uracil catabolic process

GO:0019740; Biological process:nitrogen utilization

Cross-references [?]

Pfam	PF00296; Bac_luciferase; 1;
NCBIfam	TIGR03612; RutA; 1;

Features [?]

From: RUTA_ECOLI (P75898)

Key

From

Description

Tag

Condition

FTGroup

BINDING