HAMAP rule MF_01699

General rule information [?]

Accession	MF_01699
Dates	2-NOV-2010 (Created) 1-JUN-2023 (Last updated, Version 15)

Name

RutA

Scope

Bacteria

Template

P75898 (RUTA_ECOLI)

Triggered by

HAMAP; MF_01699 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

RUTA

Protein name

RecName:		Full=Pyrimidine monooxygenase RutA;
		EC 1.14.99.46;

Gene name

rutA

Comments [?]

Function	Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen atoms in the process to yield ureidoacrylate peracid, that immediately reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN-N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the flavin reductase RutF to regenerate FMN in vivo.
Catalytic activity	RHEA:31587: FMNH2 + NADH + O2 + uracil = (Z)-3-ureidoacrylate + FMN + H(+) + H2O + NAD(+) EC 1.14.99.46
	RHEA:31599: FMNH2 + NADH + O2 + thymine = (Z)-2-methylureidoacrylate + FMN + H(+) + H2O + NAD(+) EC 1.14.99.46

case <OC:Escherichia> or <OC:Shigella>

Induction

Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.

end case

Similarity

Belongs to the NtaA/SnaA/DszA monooxygenase family. RutA subfamily.

Keywords [?]

Flavoprotein
FMN
Monooxygenase
NADP
Oxidoreductase

Gene Ontology [?]

GO:0004497; Molecular function: monooxygenase activity.
GO:0006212; Biological process: uracil catabolic process.
GO:0019740; Biological process: nitrogen utilization.

Cross-references [?]

Pfam	PF00296; Bac_luciferase; 1;
NCBIfam	TIGR03612; RutA; 1;

Features [?]

From: RUTA_ECOLI (P75898)

Key From To Description Tag Condition FTGroup

BINDING 68 69 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 I-K

BINDING 159 160 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 R-Y

BINDING 134 134 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 N

BINDING 143 143 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 E

BINDING 209 209 /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210 S

Additional information [?]

Size range	331-393 amino acids
Related rules	None
Fusion	None

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