HAMAP rule MF_01699
General rule information
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Accession | MF_01699 |
Dates | 2-NOV-2010 (Created)
28-NOV-2023 (Last updated, Version 16) |
Name | RutA |
Scope(s) |
Bacteria |
Template(s) | P75898 (RUTA_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_01699 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | RUTA |
Protein name | RecName: Full=Pyrimidine monooxygenase RutA; EC=1.14.99.46; |
Gene name | Name=rutA; |
Comments
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FUNCTION | Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen atoms in the process to yield ureidoacrylate peracid, that immediately reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN- N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the flavin reductase RutF to regenerate FMN in vivo. |
CATALYTIC ACTIVITY | Reaction=FMNH2 + NADH + O2 + uracil = (Z)-3-ureidoacrylate + FMN + H(+) + H2O + NAD(+); Xref=Rhea:RHEA:31587, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17568, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618, ChEBI:CHEBI:57945, ChEBI:CHEBI:58210, ChEBI:CHEBI:59891; EC=1.14.99.46; |
CATALYTIC ACTIVITY | Reaction=FMNH2 + NADH + O2 + thymine = (Z)-2-methylureidoacrylate + FMN + H(+) + H2O + NAD(+); Xref=Rhea:RHEA:31599, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17821, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618, ChEBI:CHEBI:57945, ChEBI:CHEBI:58210, ChEBI:CHEBI:143783; EC=1.14.99.46; |
case <OC:Escherichia> or <OC:Shigella> | |
INDUCTION | Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR. |
end case | |
SIMILARITY | Belongs to the NtaA/SnaA/DszA monooxygenase family. RutA subfamily. |
Keywords
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Gene Ontology
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GO:0004497; Molecular function:monooxygenase activity |
GO:0006212; Biological process:uracil catabolic process |
GO:0019740; Biological process:nitrogen utilization |
Cross-references
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Features
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From: RUTA_ECOLI (P75898) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 68 | 69 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
I-K | ||||||||
BINDING | 159 | 160 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
R-Y | ||||||||
BINDING | 134 | 134 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
N | ||||||||
BINDING | 143 | 143 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
E | ||||||||
BINDING | 209 | 209 | /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210" |
S |
Additional information
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Size range | 331-393 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |