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HAMAP rule MF_01699

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General rule information [?]

Accession MF_01699
Dates 2-NOV-2010 (Created)
1-JUN-2023 (Last updated, Version 15)
Name RutA
Template P75898 (RUTA_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Pyrimidine monooxygenase RutA;
Gene name

Comments [?]

Function Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen atoms in the process to yield ureidoacrylate peracid, that immediately reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN-N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the flavin reductase RutF to regenerate FMN in vivo.
Catalytic activity RHEA:31587: FMNH2 + NADH + O2 + uracil = (Z)-3-ureidoacrylate + FMN + H(+) + H2O + NAD(+)
RHEA:31599: FMNH2 + NADH + O2 + thymine = (Z)-2-methylureidoacrylate + FMN + H(+) + H2O + NAD(+)
case <OC:Escherichia> or <OC:Shigella>
Induction Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.
end case
Similarity Belongs to the NtaA/SnaA/DszA monooxygenase family. RutA subfamily.

Keywords [?]

Gene Ontology [?]

GO:0004497; Molecular function: monooxygenase activity.
GO:0006212; Biological process: uracil catabolic process.
GO:0019740; Biological process: nitrogen utilization.

Cross-references [?]

Pfam PF00296; Bac_luciferase; 1;
NCBIfam TIGR03612; RutA; 1;

Features [?]

From: RUTA_ECOLI (P75898)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     68     69       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     I-K  
BINDING     159     160       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     R-Y  
BINDING     134     134       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     N  
BINDING     143     143       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     E  
BINDING     209     209       /ligand="FMN" /ligand_id="ChEBI:CHEBI:58210     S  

Additional information [?]

Size range 331-393 amino acids
Related rules None
Fusion None