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Annotation rule MF_01699 |
General rule information
[?]
Accession |
MF_01699 |
Dates |
2-NOV-2010 (Created) 7-JAN-2020 (Last updated, Version 11) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Function |
Catalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen atoms in the process to yield ureidoacrylate peracid, that immediately reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN-N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the flavin reductase RutF to regenerate FMN in vivo. |
Catalytic activity |
RHEA:31587: FMNH2 + NADH + O2 + uracil = (Z)-3-ureidoacrylate + FMN + H(+) + H2O + NAD(+)
EC 1.14.99.46
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RHEA:31599: FMNH2 + NADH + O2 + thymine = (Z)-2-methylureidoacrylate + FMN + H(+) + H2O + NAD(+)
EC 1.14.99.46
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case <OC:Escherichia> or <OC:Shigella>
Induction |
Up-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR. |
end case
Similarity |
Belongs to the NtaA/SnaA/SoxA(DszA) monooxygenase family. RutA subfamily. |
GO:0004497; Molecular function: monooxygenase activity.
GO:0006212; Biological process: uracil catabolic process.
GO:0019740; Biological process: nitrogen utilization.
From: RUTA_ECOLI (P75898) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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NP_BIND
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68
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69
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FMN
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I-K
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NP_BIND
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159
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160
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FMN
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R-Y
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BINDING
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134
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134
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FMN
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N
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BINDING
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143
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143
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FMN
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E
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BINDING
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209
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209
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FMN
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S
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Additional information
[?]
Size range |
331-393 amino acids |
Related rules |
None |
Fusion |
None |