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HAMAP rule MF_01699

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General rule information [?]

Accession MF_01699
Dates 2-NOV-2010 (Created)
28-NOV-2023 (Last updated, Version 16)
Name RutA
Scope(s) Bacteria
Template(s) P75898 (RUTA_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01699 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RUTA
Protein name RecName: Full=Pyrimidine monooxygenase RutA;
                 EC=1.14.99.46;
Gene name Name=rutA;

Comments [?]

FUNCTIONCatalyzes the pyrimidine ring opening between N-3 and C-4 by an unusual flavin hydroperoxide-catalyzed mechanism, adding oxygen atoms in the process to yield ureidoacrylate peracid, that immediately reacts with FMN forming ureidoacrylate and FMN-N(5)-oxide. The FMN- N(5)-oxide reacts spontaneously with NADH to produce FMN. Requires the flavin reductase RutF to regenerate FMN in vivo.
CATALYTIC ACTIVITY Reaction=FMNH2 + NADH + O2 + uracil = (Z)-3-ureidoacrylate + FMN + H(+) + H2O + NAD(+); Xref=Rhea:RHEA:31587, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17568, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618, ChEBI:CHEBI:57945, ChEBI:CHEBI:58210, ChEBI:CHEBI:59891; EC=1.14.99.46;
CATALYTIC ACTIVITY Reaction=FMNH2 + NADH + O2 + thymine = (Z)-2-methylureidoacrylate + FMN + H(+) + H2O + NAD(+); Xref=Rhea:RHEA:31599, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17821, ChEBI:CHEBI:57540, ChEBI:CHEBI:57618, ChEBI:CHEBI:57945, ChEBI:CHEBI:58210, ChEBI:CHEBI:143783; EC=1.14.99.46;
case <OC:Escherichia> or <OC:Shigella>
INDUCTIONUp-regulated by the nitrogen regulatory protein C (NtrC also called GlnG) and repressed by RutR.
end case
SIMILARITYBelongs to the NtaA/SnaA/DszA monooxygenase family. RutA subfamily.

Keywords [?]


Gene Ontology [?]

GO:0004497; Molecular function:monooxygenase activity
GO:0006212; Biological process:uracil catabolic process
GO:0019740; Biological process:nitrogen utilization

Cross-references [?]

Pfam PF00296; Bac_luciferase; 1;
NCBIfam TIGR03612; RutA; 1;

Features [?]

From: RUTA_ECOLI (P75898)
Key From To Description Tag Condition FTGroup
BINDING 68 69 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
I-K
BINDING 159 160 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
R-Y
BINDING 134 134 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
N
BINDING 143 143 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
E
BINDING 209 209 /ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"
S

Additional information [?]

Size range 331-393 amino acids
Related rules None
Fusion Nter: None Cter: None



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