HAMAP rule MF_01808
General rule information
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| Accession | MF_01808 |
| Dates | 17-AUG-2003 (Created) 1-JUN-2023 (Last updated, Version 37) |
| Name | Recomb_XerC_XerD |
| Scope | Bacteria |
| Templates | P0A8P6 (XERC_ECOLI); P44818 (XERC_HAEIN); Q51566 (XERC_PSEAE): [Recover all] |
| Triggered by |
Propagated annotation
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Identifier, protein and gene names
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| Identifier |
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| Protein name |
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| Gene name |
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Comments
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case <OC:Enterobacterales>
| Function | Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. Binds cooperatively to specific DNA consensus sequences that are separated from XerD binding sites by a short central region, forming the heterotetrameric XerC-XerD complex that recombines DNA substrates. The complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids. In the complex XerC specifically exchanges the top DNA strands. |
| Activity regulation | FtsK may regulate the catalytic switch between XerC and XerD in the heterotetrameric complex during the two steps of the recombination process. |
| Subunit | Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD, in which XerC interacts with XerD via its C-terminal region, XerD interacts with XerC via its C-terminal region and so on. |
else
| Function | Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The XerC-XerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell division. It also contributes to the segregational stability of plasmids. |
| Subunit | Forms a cyclic heterotetrameric complex composed of two molecules of XerC and two molecules of XerD. |
end case
| Subcellular location | Cytoplasm. |
| Similarity | Belongs to the 'phage' integrase family. XerC subfamily. |
Keywords
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Cell cycle
Cell division
Chromosome partition
Cytoplasm
DNA integration
DNA recombination
DNA-binding
Cell division
Chromosome partition
Cytoplasm
DNA integration
DNA recombination
DNA-binding
Gene Ontology
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GO:0009037; Molecular function: tyrosine-based site-specific recombinase activity.
GO:0007059; Biological process: chromosome segregation.
GO:0006310; Biological process: DNA recombination.
GO:0006313; Biological process: DNA transposition.
GO:0005737; Cellular component: cytoplasm.
GO:0007059; Biological process: chromosome segregation.
GO:0006310; Biological process: DNA recombination.
GO:0006313; Biological process: DNA transposition.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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| Pfam | PF00589; Phage_integrase; 1; |
| PF02899; Phage_integr_N; 1; | |
| NCBIfam | TIGR02224; Recomb_XerC; 1; |
| PROSITE | PS51900; CB; 1; trigger=PRU01248; |
| PS51898; TYR_RECOMBINASE; 1; trigger=PRU01246; |
Features
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| From: XERC_ECOLI (P0A8P6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 148 | 148 | R | |||||||||
| ACT_SITE | 172 | 172 | K | |||||||||
| ACT_SITE | 240 | 240 | H | |||||||||
| ACT_SITE | 243 | 243 | R | |||||||||
| ACT_SITE | 266 | 266 | H | |||||||||
| ACT_SITE | 275 | 275 | O-(3'-phospho-DNA)-tyrosine intermediate | Y | ||||||||
Additional information
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| Size range | 250-356 amino acids |
| Related rules | MF_01807 (XERD supersedes the current rule) |
| Fusion | None |
| Comments | XerC is highly related to XerD and it is therefore difficult to discriminate them correctly. Most XerD proteins however contain a [ST]RQ tripeptide that allow to distinguish them from XerC. Entries that are matched by MF_01808 profile but not by MF_01807 are annotated as XerC. Entries that are matched by MF_01808 and MF_01807 profiles are annotated as XerD. |