HAMAP rule MF_01831
General rule information
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| Accession | MF_01831 |
| Dates | 30-SEP-2004 (Created)
2-SEP-2024 (Last updated, Version 27) |
| Name | SufS_aminotrans_5 |
| Scope(s) |
Bacteria Enterobacterales |
| Template(s) | P77444 (SUFS_ECOLI); Q9EXP2 (SUFS_DICD3); [ Recover all ] |
| Triggered by |
HAMAP; MF_01831 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SUFS |
| Protein name | RecName: Full=Cysteine desulfurase; EC=2.8.1.7; AltName: Full=Selenocysteine beta-lyase; Short=SCL; AltName: Full=Selenocysteine lyase; EC=4.4.1.16; AltName: Full=Selenocysteine reductase; |
| Gene name | Name=sufS; |
Comments
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| FUNCTION | Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L- selenocysteine. Selenocysteine lyase activity is however unsure in vivo. |
| CATALYTIC ACTIVITY | Reaction=[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L- alanine; Xref=Rhea:RHEA:43892, Rhea:RHEA-COMP:14737, Rhea:RHEA- COMP:14739, ChEBI:CHEBI:29917, ChEBI:CHEBI:35235, ChEBI:CHEBI:57972, ChEBI:CHEBI:64428; EC=2.8.1.7; |
| CATALYTIC ACTIVITY | Reaction=L-selenocysteine + AH2 = hydrogenselenide + L-alanine + A + H(+); Xref=Rhea:RHEA:11632, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29317, ChEBI:CHEBI:57843, ChEBI:CHEBI:57972; EC=4.4.1.16; |
| COFACTOR | Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; |
| PATHWAY | Cofactor biosynthesis; iron-sulfur cluster biosynthesis. |
| SUBUNIT | Homodimer. Interacts with SufE and the SufBCD complex composed of SufB, SufC and SufD. The interaction with SufE is required to mediate the direct transfer of the sulfur atom from the S- sulfanylcysteine. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily. |
Keywords
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Gene Ontology
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| GO:0031071; Molecular function:cysteine desulfurase activity |
| GO:0009000; Molecular function:selenocysteine lyase activity |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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Features
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| From: SUFS_ECOLI (P77444) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 364 | 364 | /note="Cysteine persulfide intermediate" | C | ||||||||
| MOD_RES | 226 | 226 | /note="N6-(pyridoxal phosphate)lysine" | K | ||||||||
Additional information
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| Size range | 406-412 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | The suf operon encodes a minor pathway of Fe-S cluster, which is probably activated and required only in specific stressfull conditions such as oxidative stress and/or iron limitation. Although cysteine desulfurase (EC 2.8.1.7) and selenocysteine lyase (EC 4.4.1.16) activities are reported as unrelated enzymatic reactions, they probably represent a same type of reaction, using either L-cysteine or L-selenocysteine as substrate, respectively. The catalytic activity for cysteine desulfurase reaction is incomplete, and lacks the direct transfer of the sulfur atom from the sufS-S-sulfanylcysteine |