HAMAP logo

HAMAP rule MF_01831

Send feedback

General rule information [?]

Accession MF_01831
Dates 30-SEP-2004 (Created)
1-JUN-2023 (Last updated, Version 26)
Name SufS_aminotrans_5
Scope(s) Bacteria
Template(s) P77444 (SUFS_ECOLI); Q9EXP2 (SUFS_DICD3); [ Recover all ]
Triggered by HAMAP; MF_01831 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier SUFS
Protein name RecName: Full=Cysteine desulfurase;
AltName: Full=Selenocysteine beta-lyase;
AltName: Full=Selenocysteine lyase;
AltName: Full=Selenocysteine reductase;
Gene name Name=sufS;

Comments [?]

FUNCTIONCysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L- selenocysteine. Selenocysteine lyase activity is however unsure in vivo.
CATALYTIC ACTIVITY Reaction=[sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L- alanine; Xref=Rhea:RHEA:43892, Rhea:RHEA-COMP:14737, Rhea:RHEA- COMP:14739, ChEBI:CHEBI:29917, ChEBI:CHEBI:35235, ChEBI:CHEBI:57972, ChEBI:CHEBI:64428; EC=;
CATALYTIC ACTIVITY Reaction=AH2 + L-selenocysteine = A + H(+) + hydrogenselenide + L- alanine; Xref=Rhea:RHEA:11632, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29317, ChEBI:CHEBI:57843, ChEBI:CHEBI:57972; EC=;
COFACTOR Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
PATHWAYCofactor biosynthesis; iron-sulfur cluster biosynthesis.
SUBUNITHomodimer. Interacts with SufE and the SufBCD complex composed of SufB, SufC and SufD. The interaction with SufE is required to mediate the direct transfer of the sulfur atom from the S- sulfanylcysteine.
SIMILARITYBelongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.

Keywords [?]

Gene Ontology [?]

GO:0031071; Molecular function:cysteine desulfurase activity
GO:0009000; Molecular function:selenocysteine lyase activity
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00266; Aminotran_5; 1;
NCBIfam TIGR01979; SufS; 1;

Features [?]

From: SUFS_ECOLI (P77444)
Key From To Description Tag Condition FTGroup
ACT_SITE 364 364 /note="Cysteine persulfide intermediate" C
MOD_RES 226 226 /note="N6-(pyridoxal phosphate)lysine" K

Additional information [?]

Size range 406-412 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments The suf operon encodes a minor pathway of Fe-S cluster, which is probably activated and required only in specific stressfull conditions such as oxidative stress and/or iron limitation. Although cysteine desulfurase (EC and selenocysteine lyase (EC activities are reported as unrelated enzymatic reactions, they probably represent a same type of reaction, using either L-cysteine or L-selenocysteine as substrate, respectively. The catalytic activity for cysteine desulfurase reaction is incomplete, and lacks the direct transfer of the sulfur atom from the sufS-S-sulfanylcysteine

View rule in raw text format (no links)