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HAMAP rule MF_01831

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General rule information [?]

Accession MF_01831
Dates 30-SEP-2004 (Created)
1-JUN-2023 (Last updated, Version 26)
Name SufS_aminotrans_5
Bacteria; Enterobacterales
Templates P77444 (SUFS_ECOLI); Q9EXP2 (SUFS_DICD3): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Cysteine desulfurase;
AltName: Full=Selenocysteine beta-lyase;
AltName: Full=Selenocysteine lyase;
AltName: Full=Selenocysteine reductase;
Gene name

Comments [?]

Function Cysteine desulfurases mobilize the sulfur from L-cysteine to yield L-alanine, an essential step in sulfur metabolism for biosynthesis of a variety of sulfur-containing biomolecules. Component of the suf operon, which is activated and required under specific conditions such as oxidative stress and iron limitation. Acts as a potent selenocysteine lyase in vitro, that mobilizes selenium from L-selenocysteine. Selenocysteine lyase activity is however unsure in vivo.
Catalytic activity RHEA:43892: [sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine
RHEA:11632: AH2 + L-selenocysteine = A + H(+) + hydrogenselenide + L-alanine
Cofactor pyridoxal 5'-phosphate
Pathway Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
Subunit Homodimer. Interacts with SufE and the SufBCD complex composed of SufB, SufC and SufD. The interaction with SufE is required to mediate the direct transfer of the sulfur atom from the S-sulfanylcysteine.
Subcellular location Cytoplasm.
Similarity Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily.

Keywords [?]

Gene Ontology [?]

GO:0031071; Molecular function: cysteine desulfurase activity.
GO:0009000; Molecular function: selenocysteine lyase activity.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00266; Aminotran_5; 1;
NCBIfam TIGR01979; SufS; 1;

Features [?]

From: SUFS_ECOLI (P77444)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     364     364       Cysteine persulfide intermediate     C  
MOD_RES     226     226       N6-(pyridoxal phosphate)lysine     K  

Additional information [?]

Size range 406-412 amino acids
Related rules None
Fusion None
Comments The suf operon encodes a minor pathway of Fe-S cluster, which is probably activated and required only in specific stressfull conditions such as oxidative stress and/or iron limitation. Although cysteine desulfurase (EC and selenocysteine lyase (EC activities are reported as unrelated enzymatic reactions, they probably represent a same type of reaction, using either L-cysteine or L-selenocysteine as substrate, respectively. The catalytic activity for cysteine desulfurase reaction is incomplete, and lacks the direct transfer of the sulfur atom from the sufS-S-sulfanylcysteine