HAMAP rule MF_01833
General rule information
[?]
Accession |
MF_01833 |
Dates |
30-SEP-2004 (Created) 28-APR-2022 (Last updated, Version 14) |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
|
Full=tRNA-splicing endonuclease;
|
|
EC 4.6.1.16;
|
AltName:
|
Full=tRNA-intron endonuclease;
|
|
Function |
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. |
Catalytic activity |
Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16; |
Subunit |
Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers. |
Similarity |
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily. |
GO:0000213; Molecular function: tRNA-intron endonuclease activity.
GO:0006388; Biological process: tRNA splicing, via endonucleolytic cleavage and ligation.
From: ENDA_METJA (Q58819) |
Key
|
|
From
|
|
To
|
|
Description
|
|
Tag
|
|
Condition
|
|
FTGroup
|
ACT_SITE
|
|
115
|
|
115
|
|
|
|
|
|
Y
|
|
|
ACT_SITE
|
|
125
|
|
125
|
|
|
|
|
|
H
|
|
|
ACT_SITE
|
|
156
|
|
156
|
|
|
|
|
|
K
|
|
|
Additional information
[?]
Size range |
154-187 amino acids |
Related rules |
MF_01834 (ENDA) |
Fusion |
None |
Comments |
In archaea, the tRNA-intron endonuclease enzyme is either composed of a homotetramer of a protein of 150-190 amino acids (this family), or composed of a homodimer of a endA protein of 300-370 amino acids (MF_01834), in which each protein of the dimer arose by tandem duplication of the endA protein of 150-190 amino acids. |