HAMAP logo

HAMAP rule MF_01833

Send feedback

General rule information [?]

Accession MF_01833
Dates 30-SEP-2004 (Created)
1-JUN-2023 (Last updated, Version 15)
Name EndA_short
Scope(s) Archaea
Template(s) Q58819 (ENDA_METJA); [ Recover all ]
Triggered by HAMAP; MF_01833 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ENDA
Protein name RecName: Full=tRNA-splicing endonuclease;
AltName: Full=tRNA-intron endonuclease;
Gene name Name=endA;

Comments [?]

FUNCTIONEndonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
CATALYTIC ACTIVITY Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'- half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=;
SUBUNITHomotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.
SIMILARITYBelongs to the tRNA-intron endonuclease family. Archaeal short subfamily.

Keywords [?]

Gene Ontology [?]

GO:0000213; Molecular function:tRNA-intron endonuclease activity
GO:0006388; Biological process:tRNA splicing, via endonucleolytic cleavage and ligation

Cross-references [?]

Pfam PF01974; tRNA_int_endo; 1;
Pfam PF02778; tRNA_int_endo_N; 1;
NCBIfam TIGR00324; EndA; 1;

Features [?]

From: ENDA_METJA (Q58819)
Key From To Description Tag Condition FTGroup
ACT_SITE 115 115 Y
ACT_SITE 125 125 H
ACT_SITE 156 156 K

Additional information [?]

Size range 154-187 amino acids
Related rules MF_01834
Fusion Nter: None Cter: None
Comments In archaea, the tRNA-intron endonuclease enzyme is either composed of a homotetramer of a protein of 150-190 amino acids (this family), or composed of a homodimer of a endA protein of 300-370 amino acids (MF_01834), in which each protein of the dimer arose by tandem duplication of the endA protein of 150-190 amino acids.

View rule in raw text format (no links)