HAMAP rule MF_01833
General rule information
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| Accession |
MF_01833 |
| Dates |
30-SEP-2004 (Created)
1-JUN-2023 (Last updated, Version 15) |
Propagated annotation
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Identifier, protein and gene names
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| Protein name |
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RecName:
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Full=tRNA-splicing endonuclease;
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EC 4.6.1.16;
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AltName:
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Full=tRNA-intron endonuclease;
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| Function |
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. |
| Catalytic activity |
Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16; |
| Subunit |
Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers. |
| Similarity |
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily. |
GO:0000213; Molecular function: tRNA-intron endonuclease activity.
GO:0006388; Biological process: tRNA splicing, via endonucleolytic cleavage and ligation.
| From: ENDA_METJA (Q58819) |
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Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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ACT_SITE
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115
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115
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Y
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ACT_SITE
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125
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125
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H
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ACT_SITE
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156
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156
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K
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Additional information
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| Size range |
154-187 amino acids |
| Related rules |
MF_01834 (ENDA) |
| Fusion |
None |
| Comments |
In archaea, the tRNA-intron endonuclease enzyme is either composed of a homotetramer of a protein of 150-190 amino acids (this family), or composed of a homodimer of a endA protein of 300-370 amino acids (MF_01834), in which each protein of the dimer arose by tandem duplication of the endA protein of 150-190 amino acids. |
