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Annotation rule MF_01834
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General rule information [?]

Accession MF_01834
Dates 30-SEP-2004 (Created)
19-NOV-2019 (Last updated, Version 13)
Name EndA_long
Scope
Archaea
Templates O07118 (ENDA_HALVD); O29362 (ENDA_ARCFU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
ENDA
Protein name
RecName: Full=tRNA-splicing endonuclease;
EC 4.6.1.16;
AltName: Full=tRNA-intron endonuclease;
Gene name
endA

Comments [?]

Function Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
Catalytic activity Reaction=PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
Subunit Homodimer.
Similarity Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Keywords [?]


Gene Ontology [?]

GO:0000213; Molecular function: tRNA-intron endonuclease activity.
GO:0006388; Biological process: tRNA splicing, via endonucleolytic cleavage and ligation.

Cross-references [?]

Pfam PF01974; tRNA_int_endo; 1;
PF02778; tRNA_int_endo_N; 1;
TIGRFAMs TIGR00324; EndA; 1;

Features [?]

From: ENDA_HALVD (O07118)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     274     274             Y  
ACT_SITE     285     285             H  
ACT_SITE     316     316             K  

Additional information [?]

Size range 289-353 amino acids
Related rules MF_01833 (ENDA supersedes the current rule)
Fusion None
Comments In archaea, the tRNA-intron endonuclease enzyme is either composed of a homotetramer of a protein of 150-190 amino acids (MF_01833), or composed of a homodimer of a endA protein of 300-370 amino acids (this family), in which each protein of the dimer arose by tandem duplication of the endA protein of 150-190 amino acids.