HAMAP rule MF_01834

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01834
Accession	MF_01834
Dates	28-FEB-2005 (Created) 12-JUN-2025 (Last updated, Version 17)
Name	EndA_long
Scope(s)	Archaea
Template(s)	O07118; O29362; [ Recover all ]
Triggered by	HAMAP; MF_01834 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	ENDA
Protein name	RecName: Full=tRNA-splicing endonuclease; EC=4.6.1.16; AltName: Full=tRNA-intron endonuclease;
Gene name	Name=endA;

Comments [?]

FUNCTION	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.
CATALYTIC ACTIVITY	Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'- half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
SUBUNIT	Homodimer.
SIMILARITY	Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Keywords [?]

Gene Ontology [?]

GO:0000213; Molecular function:tRNA-intron lyase activity

GO:0006388; Biological process:tRNA splicing, via endonucleolytic cleavage and ligation

Cross-references [?]

Features [?]

From: ENDA_HALVD (O07118)

Key

From

Description

Tag

Condition

FTGroup

ACT_SITE

274

ACT_SITE

285

ACT_SITE

316

Additional information [?]

Size range	289-353 amino acids
Related rules	MF_01833
Fusion	Nter: None Cter: None
Comments	In archaea, the tRNA-intron endonuclease enzyme is either composed of a homotetramer of a protein of 150-190 amino acids (MF_01833), or composed of a homodimer of a endA protein of 300-370 amino acids (this family), in which each protein of the dimer arose by tandem duplication of the endA protein of 150-190 amino acids.