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Annotation rule MF_01836 |
General rule information
[?]
Accession |
MF_01836 |
Dates |
13-SEP-2004 (Created) 19-NOV-2019 (Last updated, Version 23) |
Scope |
Bacteria; Cyanobacteria |
Propagated annotation
[?]
Identifier, protein and gene names
[?]
Protein name |
RecName:
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Full=Circadian clock protein kinase KaiC;
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EC 2.7.11.1;
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case not <OC:Prochloraceae>
Function |
Core component of the KaiABC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiABC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. |
Catalytic activity |
RHEA:17989: ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
EC 2.7.11.1
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RHEA:46608: ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]
EC 2.7.11.1
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Cofactor |
Mg(2+) |
Activity regulation |
The interaction with KaiA enhances its phosphorylation status, while the interaction with KaiB decreases it. A KaiA dimer is sufficient to enhance KaiC phosphorylation. |
Subunit |
Homohexamer; hexamerization is dependent on ATP-binding. Core component of the KaiABC complex, at least composed of a KaiC homohexamer, a KaiB dimer and two KaiA dimers. Interacts directly with SasA. |
Domain |
The KaiC domains mediate the interaction with KaiA. |
Ptm |
Phosphorylated on serine/threonine residues by autocatalysis. Both phosphorylated and unphosphorylated forms exist. Can probably autophosphorylate and autodephosphorylate. Phosphorylated form correlates with clock speed. |
end case
case <OC:Prochloraceae>
Function |
Core component of the KaiBC clock protein complex, which constitutes the main circadian regulator in cyanobacteria. Binds to DNA. The KaiBC complex may act as a promoter-nonspecific transcription regulator that represses transcription, possibly by acting on the state of chromosome compaction. |
Catalytic activity |
RHEA:17989: ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
EC 2.7.11.1
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|
RHEA:46608: ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]
EC 2.7.11.1
|
Cofactor |
Mg(2+) |
Subunit |
Homohexamer; hexamerization is dependent on ATP-binding. Component of the KaiBC complex with KaiB. Interacts directly with SasA. |
Ptm |
Phosphorylated on serine/threonine residues by autocatalysis. Both phosphorylated and unphosphorylated forms exist. Can probably autophosphorylate and autodephosphorylate. Phosphorylated form correlates with clock speed. |
end case
Similarity |
Belongs to the KaiC family. |
GO:0000287; Molecular function: magnesium ion binding.
GO:0004712; Molecular function: protein serine/threonine/tyrosine kinase activity.
GO:0005524; Molecular function: ATP binding.
GO:0007623; Biological process: circadian rhythm.
From: KAIC_SYNE7 (Q79PF4) |
Key
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From
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To
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Description
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Tag
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Condition
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FTGroup
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DOMAIN
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72
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180
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KaiC 1
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DOMAIN
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313
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412
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KaiC 2
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NP_BIND
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46
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53
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ATP
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G-x-[SAT]-G-x-G-K-[ST]
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NP_BIND
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288
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295
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ATP
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G-x-[SAT]-G-x-G-K-[ST]
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METAL
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295
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295
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Magnesium
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T
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METAL
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318
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318
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Magnesium
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E
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METAL
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319
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319
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Magnesium
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E
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METAL
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378
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378
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Magnesium
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D
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MOD_RES
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432
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432
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Phosphothreonine; by autocatalysis
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T
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Additional information
[?]
Size range |
499-541 amino acids |
Related rules |
None |
Fusion |
None |
Comments |
Prochlorales do not contain the KaiA protein, which constitutes a regulator of KaiC, suggesting that regulation of circadian cycle is different in these bacteria. |