HAMAP rule MF_01850
General rule information
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Accession | MF_01850 |
Dates | 15-JUL-2008 (Created) 17-FEB-2023 (Last updated, Version 16) |
Name | TtcA |
Scope | Bacteria; Bacteroidota
Bacteria; Acaryochloris
Bacteria; Deinococcota
Bacteria; Pseudomonadota |
Templates | P76055 (TTCA_ECOLI); Q8ZP88 (TTCA_SALTY): [Recover all] |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Catalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system. |
Catalytic activity | RHEA:57048: AH2 + ATP + cytidine(32) in tRNA + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = 2-thiocytidine(32) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[cysteine desulfurase]
PhysiologicalDirection=left-to-right (RHEA:57049) |
Cofactor | [4Fe-4S] cluster Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues, the fourth Fe has a free coordination site that may bind a sulfur atom transferred from the persulfide of IscS |
Mg(2+) | |
Pathway | tRNA modification. |
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Miscellaneous | The thiolation reaction likely consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by the hydrosulfide attached to the Fe-S cluster. |
Similarity | Belongs to the TtcA family. |
Keywords
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4Fe-4S
ATP-binding
Cytoplasm
Iron
Iron-sulfur
Magnesium
Metal-binding
Nucleotide-binding
RNA-binding
Transferase
tRNA processing
tRNA-binding
ATP-binding
Cytoplasm
Iron
Iron-sulfur
Magnesium
Metal-binding
Nucleotide-binding
RNA-binding
Transferase
tRNA processing
tRNA-binding
Gene Ontology
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GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.
GO:0005524; Molecular function: ATP binding.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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Pfam | PF01171; ATP_bind_3; 1; |
PIRSF | PIRSF004976; ATPase_YdaO; 1; |
Features
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From: TTCA_ECOLI (P76055) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
MOTIF | 47 | 52 | PP-loop motif | S-G-G-K-D-S | ||||||||
BINDING | 122 | 122 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 | |||||||
BINDING | 125 | 125 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 | |||||||
BINDING | 213 | 213 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 | C | 1 |
Additional information
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Size range | 251-381 amino acids |
Related rules | None |
Fusion | None |