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HAMAP rule MF_01850

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General rule information [?]

Accession MF_01850
Dates 15-JUL-2008 (Created)
17-FEB-2023 (Last updated, Version 16)
Name TtcA
Scope(s) Bacteria
Template(s) P76055 (TTCA_ECOLI); Q8ZP88 (TTCA_SALTY); [ Recover all ]
Triggered by HAMAP; MF_01850 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TTCA
Protein name RecName: Full=tRNA-cytidine(32) 2-sulfurtransferase;
AltName: Full=Two-thiocytidine biosynthesis protein A;
AltName: Full=tRNA 2-thiocytidine biosynthesis protein TtcA;
Gene name Name=ttcA;

Comments [?]

FUNCTIONCatalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system.
CATALYTIC ACTIVITY Reaction=AH2 + ATP + cytidine(32) in tRNA + S-sulfanyl-L-cysteinyl- [cysteine desulfurase] = 2-thiocytidine(32) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[cysteine desulfurase]; Xref=Rhea:RHEA:57048, Rhea:RHEA-COMP:10288, Rhea:RHEA-COMP:12157, Rhea:RHEA-COMP:12158, Rhea:RHEA-COMP:14821, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:61963, ChEBI:CHEBI:82748, ChEBI:CHEBI:141453, ChEBI:CHEBI:456215; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57049;
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues, the fourth Fe has a free coordination site that may bind a sulfur atom transferred from the persulfide of IscS
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYtRNA modification.
MISCELLANEOUSThe thiolation reaction likely consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by the hydrosulfide attached to the Fe-S cluster.
SIMILARITYBelongs to the TtcA family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0005524; Molecular function:ATP binding
GO:0016783; Molecular function:sulfurtransferase activity
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding
GO:0034227; Biological process:tRNA thio-modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01171; ATP_bind_3; 1;
PIRSF PIRSF004976; ATPase_YdaO; 1;

Features [?]

From: TTCA_ECOLI (P76055)
Key From To Description Tag Condition FTGroup
MOTIF 47 52 /note="PP-loop motif" S-G-G-K-D-S
BINDING 122 122 /ligand="[4Fe-4S] cluster"
C 1
BINDING 125 125 /ligand="[4Fe-4S] cluster"
C 1
BINDING 213 213 /ligand="[4Fe-4S] cluster"
C 1

Additional information [?]

Size range 251-381 amino acids
Related rules None
Fusion Nter: None Cter: None

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