HAMAP rule MF_01850

General rule information [?]

Accession	MF_01850
Dates	15-JUL-2008 (Created) 17-FEB-2023 (Last updated, Version 16)

Name

TtcA

Scope

Bacteria; Bacteroidota

Bacteria; Acaryochloris

Bacteria; Deinococcota

Bacteria; Pseudomonadota

Templates

P76055 (TTCA_ECOLI); Q8ZP88 (TTCA_SALTY): [Recover all]

Triggered by

HAMAP; MF_01850 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

TTCA

Protein name

RecName:		Full=tRNA-cytidine(32) 2-sulfurtransferase;
		EC 2.8.1.-;
AltName:		Full=Two-thiocytidine biosynthesis protein A;
AltName:		Full=tRNA 2-thiocytidine biosynthesis protein TtcA;

Gene name

ttcA

Comments [?]

Function	Catalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system.
Catalytic activity	RHEA:57048: AH2 + ATP + cytidine(32) in tRNA + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] = 2-thiocytidine(32) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[cysteine desulfurase] PhysiologicalDirection=left-to-right (RHEA:57049)
Cofactor	[4Fe-4S] cluster Note: Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues, the fourth Fe has a free coordination site that may bind a sulfur atom transferred from the persulfide of IscS
	Mg(2+)
Pathway	tRNA modification.
Subunit	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	The thiolation reaction likely consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by the hydrosulfide attached to the Fe-S cluster.
Similarity	Belongs to the TtcA family.

Keywords [?]

4Fe-4S
ATP-binding
Cytoplasm
Iron
Iron-sulfur
Magnesium
Metal-binding
Nucleotide-binding
RNA-binding
Transferase
tRNA processing
tRNA-binding

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0016783; Molecular function: sulfurtransferase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0034227; Biological process: tRNA thio-modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF01171; ATP_bind_3; 1;
PIRSF	PIRSF004976; ATPase_YdaO; 1;

Features [?]

From: TTCA_ECOLI (P76055)

Key From To Description Tag Condition FTGroup

MOTIF 47 52 PP-loop motif S-G-G-K-D-S

BINDING 122 122 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C 1

BINDING 125 125 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C 1

BINDING 213 213 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883 C 1

Additional information [?]

Size range	251-381 amino acids
Related rules	None
Fusion	None

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