HAMAP rule MF_01850
General rule information
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| Accession | MF_01850 |
| Dates | 15-JUL-2008 (Created)
17-FEB-2023 (Last updated, Version 16) |
| Name | TtcA |
| Scope(s) |
Bacteria Bacteroidota Acaryochloris Deinococcota Pseudomonadota |
| Template(s) | P76055 (TTCA_ECOLI); Q8ZP88 (TTCA_SALTY); [ Recover all ] |
| Triggered by |
HAMAP; MF_01850 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TTCA |
| Protein name | RecName: Full=tRNA-cytidine(32) 2-sulfurtransferase; EC=2.8.1.-; AltName: Full=Two-thiocytidine biosynthesis protein A; AltName: Full=tRNA 2-thiocytidine biosynthesis protein TtcA; |
| Gene name | Name=ttcA; |
Comments
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| FUNCTION | Catalyzes the ATP-dependent 2-thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32). The sulfur atoms are provided by the cysteine/cysteine desulfurase (IscS) system. |
| CATALYTIC ACTIVITY | Reaction=AH2 + ATP + cytidine(32) in tRNA + S-sulfanyl-L-cysteinyl- [cysteine desulfurase] = 2-thiocytidine(32) in tRNA + A + AMP + diphosphate + H(+) + L-cysteinyl-[cysteine desulfurase]; Xref=Rhea:RHEA:57048, Rhea:RHEA-COMP:10288, Rhea:RHEA-COMP:12157, Rhea:RHEA-COMP:12158, Rhea:RHEA-COMP:14821, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17499, ChEBI:CHEBI:29950, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:61963, ChEBI:CHEBI:82748, ChEBI:CHEBI:141453, ChEBI:CHEBI:456215; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:57049; |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit. The cluster is chelated by three Cys residues, the fourth Fe has a free coordination site that may bind a sulfur atom transferred from the persulfide of IscS |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| PATHWAY | tRNA modification. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| MISCELLANEOUS | The thiolation reaction likely consists of two steps: a first activation step by ATP to form an adenylated intermediate of the target base of tRNA, and a second nucleophilic substitution step of the sulfur (S) atom supplied by the hydrosulfide attached to the Fe-S cluster. |
| SIMILARITY | Belongs to the TtcA family. |
Keywords
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| 4Fe-4S |
| ATP-binding |
| Cytoplasm |
| Iron |
| Iron-sulfur |
| Magnesium |
| Metal-binding |
| Nucleotide-binding |
| RNA-binding |
| Transferase |
| tRNA processing |
| tRNA-binding |
Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0005524; Molecular function:ATP binding |
| GO:0016783; Molecular function:sulfurtransferase activity |
| GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding |
| GO:0034227; Biological process:tRNA thio-modification |
| GO:0005737; Cellular component:cytoplasm |
Cross-references
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| Pfam | PF01171; ATP_bind_3; 1; |
| PIRSF | PIRSF004976; ATPase_YdaO; 1; |
Features
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| From: TTCA_ECOLI (P76055) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 47 | 52 | /note="PP-loop motif" | S-G-G-K-D-S | ||||||||
| BINDING | 122 | 122 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 125 | 125 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
| BINDING | 213 | 213 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 1 | |||||||
Additional information
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| Size range | 251-381 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |