HAMAP rule MF_01852
General rule information
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Accession | MF_01852 |
Dates | 23-SEP-2008 (Created) 19-NOV-2019 (Last updated, Version 13) |
Name | TsaC |
Scope | Bacteria; Betaproteobacteria
Bacteria; Gammaproteobacteria |
Template | P45748 (TSAC_ECOLI) |
Triggered by |
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
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Gene name |
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Comments
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Function | Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. |
Catalytic activity | RHEA:36407: ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate
EC 2.7.7.87 |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the SUA5 family. TsaC subfamily. |
Keywords
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Gene Ontology
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GO:0005524; Molecular function: ATP binding.
GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
GO:0005737; Cellular component: cytoplasm.
GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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PROSITE | PS51163; YRDC; 1; trigger=PRU00518; |
Pfam | PF01300; Sua5_yciO_yrdC; 1; |
TIGRFAMs | TIGR00057; TIGR00057; 1; |
Additional information
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Size range | 175-214 amino acids |
Related rules | None |
Fusion | None |
Comments | The well-known t(6)A modification appears to be a hydrolyzed artifact of natural cyclic t(6)A (ct(6)A) that occurs during the preparation and handling of tRNA in E.coli, B.subtilis and many other species including fungi and plants (PubMed:23242255). In these species, the t(6)A modification is processed further by dehydration into ct(6)A, a reaction catalyzed by TcdA. |