HAMAP rule MF_01852

General rule information [?]

Accession	MF_01852
Dates	23-SEP-2008 (Created) 19-NOV-2019 (Last updated, Version 13)

Name

TsaC

Scope

Bacteria; Betaproteobacteria

Bacteria; Gammaproteobacteria

Template

P45748 (TSAC_ECOLI)

Triggered by

HAMAP; MF_01852 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

TSAC

Protein name

RecName:		Full=Threonylcarbamoyl-AMP synthase;
		Short=TC-AMP synthase;
		EC 2.7.7.87;
AltName:		Full=L-threonylcarbamoyladenylate synthase;
AltName:		Full=tRNA threonylcarbamoyladenosine biosynthesis protein TsaC;
AltName:		Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaC;

Gene name

tsaC

Comments [?]

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate.
Catalytic activity	RHEA:36407: ATP + hydrogencarbonate + L-threonine = diphosphate + H2O + L-threonylcarbamoyladenylate EC 2.7.7.87
Subcellular location	Cytoplasm.
Similarity	Belongs to the SUA5 family. TsaC subfamily.

Keywords [?]

ATP-binding
Cytoplasm
Nucleotide-binding
Nucleotidyltransferase
Transferase
tRNA processing

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0016779; Molecular function: nucleotidyltransferase activity.
GO:0002949; Biological process: tRNA threonylcarbamoyladenosine modification.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS51163; YRDC; 1; trigger=PRU00518;
Pfam	PF01300; Sua5_yciO_yrdC; 1;
TIGRFAMs	TIGR00057; TIGR00057; 1;

Additional information [?]

Size range	175-214 amino acids
Related rules	None
Fusion	None
Comments	The well-known t(6)A modification appears to be a hydrolyzed artifact of natural cyclic t(6)A (ct(6)A) that occurs during the preparation and handling of tRNA in E.coli, B.subtilis and many other species including fungi and plants (PubMed:23242255). In these species, the t(6)A modification is processed further by dehydration into ct(6)A, a reaction catalyzed by TcdA.

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