HAMAP rule MF_01865
General rule information
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Accession | MF_01865 |
Dates | 15-APR-2009 (Created)
1-JUN-2023 (Last updated, Version 20) |
Name | MTTase_RimO |
Scope(s) |
Bacteria |
Template(s) | P0AEI4 (RIMO_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_01865 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | RIMO |
Protein name | RecName: Full=Ribosomal protein uS12 methylthiotransferase RimO; Short=uS12 MTTase; Short=uS12 methylthiotransferase; EC=2.8.4.4; AltName: Full=Ribosomal protein uS12 (aspartate-C(3))-methylthiotransferase; AltName: Full=Ribosome maturation factor RimO; |
Gene name | Name=rimO; |
Comments
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FUNCTION | Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein uS12. |
CATALYTIC ACTIVITY | Reaction=[sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L- aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:37087, Rhea:RHEA-COMP:10460, Rhea:RHEA- COMP:10461, Rhea:RHEA-COMP:14737, Rhea:RHEA-COMP:14739, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499, ChEBI:CHEBI:29917, ChEBI:CHEBI:29961, ChEBI:CHEBI:57844, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:64428, ChEBI:CHEBI:73599; EC=2.8.4.4; |
COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.; |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the methylthiotransferase family. RimO subfamily. |
Keywords
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Gene Ontology
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GO:0016740; Molecular function:transferase activity |
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding |
GO:0018339; Biological process:peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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PROSITE | PS50926; TRAM; 0-1; |
PROSITE | PS01278; MTTASE_RADICAL; 1; |
PROSITE | PS51449; MTTASE_N; 1; |
PROSITE | PS51918; RADICAL_SAM; 1; |
Pfam | PF04055; Radical_SAM; 1; |
Pfam | PF01938; TRAM; 0-1; |
Pfam | PF00919; UPF0004; 1; |
NCBIfam | TIGR01125; TIGR01125; 1; |
NCBIfam | TIGR00089; TIGR00089; 1; |
Features
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From: RIMO_ECOLI (P0AEI4) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
BINDING | 17 | 17 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
BINDING | 53 | 53 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
BINDING | 82 | 82 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" |
C | 1 | |||||||
BINDING | 150 | 150 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
BINDING | 154 | 154 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 | |||||||
BINDING | 157 | 157 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet" |
C | 2 |
Additional information
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Size range | 401-545 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |