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Annotation rule MF_01865
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General rule information [?]

Accession MF_01865
Dates 15-APR-2009 (Created)
3-APR-2020 (Last updated, Version 15)
Name MTTase_RimO
Template P0AEI4 (RIMO_ECOLI)

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Ribosomal protein S12 methylthiotransferase RimO;
Short=S12 MTTase;
Short=S12 methylthiotransferase;
AltName: Full=Ribosomal protein S12 (aspartate-C(3))-methylthiotransferase;
AltName: Full=Ribosome maturation factor RimO;
Gene name

Comments [?]

Function Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12.
Catalytic activity RHEA:37087: [sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine
Cofactor [4Fe-4S] cluster
Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Subcellular location Cytoplasm.
Similarity Belongs to the methylthiotransferase family. RimO subfamily.

Keywords [?]

Gene Ontology [?]

GO:0016740; Molecular function: transferase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0018339; Biological process: peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE PS50926; TRAM; 0-1; trigger=PRU00208;
PS51449; MTTASE_N; 1; trigger=PRU00780;
PS51918; RADICAL_SAM; 1; trigger=PRU01266;
Pfam PF04055; Radical_SAM; 1;
PF01938; TRAM; 0-1;
PF00919; UPF0004; 1;
TIGRFAMs TIGR01125; TIGR01125; 1;
TIGR00089; TIGR00089; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
METAL     17     17       Iron-sulfur (4Fe-4S)     C   1
METAL     53     53       Iron-sulfur (4Fe-4S)     C   1
METAL     82     82       Iron-sulfur (4Fe-4S)     C   1
METAL     150     150       Iron-sulfur (4Fe-4S-S-AdoMet)     C   2
METAL     154     154       Iron-sulfur (4Fe-4S-S-AdoMet)     C   2
METAL     157     157       Iron-sulfur (4Fe-4S-S-AdoMet)     C   2

Additional information [?]

Size range 401-545 amino acids
Related rules None
Fusion None