HAMAP rule MF_01865

General rule information [?]

Accession	MF_01865
Dates	15-APR-2009 (Created) 1-JUN-2023 (Last updated, Version 20)

Name	MTTase_RimO

Scope

Bacteria

Template

P0AEI4 (RIMO_ECOLI)

Triggered by

HAMAP; MF_01865 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

RIMO

Protein name

RecName:		Full=Ribosomal protein uS12 methylthiotransferase RimO;
		Short=uS12 MTTase;
		Short=uS12 methylthiotransferase;
		EC 2.8.4.4;
AltName:		Full=Ribosomal protein uS12 (aspartate-C(3))-methylthiotransferase;
AltName:		Full=Ribosome maturation factor RimO;

Gene name

rimO

Comments [?]

Function	Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein uS12.
Catalytic activity	RHEA:37087: [sulfur carrier]-SH + AH2 + L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 2 S-adenosyl-L-methionine = 3-methylsulfanyl-L-aspartate(89)-[ribosomal protein uS12]-hydrogen + 5'-deoxyadenosine + [sulfur carrier]-H + A + 2 H(+) + L-methionine + S-adenosyl-L-homocysteine EC 2.8.4.4
Cofactor	[4Fe-4S] cluster Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
Subcellular location	Cytoplasm.
Similarity	Belongs to the methylthiotransferase family. RimO subfamily.

Keywords [?]

4Fe-4S
Cytoplasm
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
Transferase

Gene Ontology [?]

GO:0016740; Molecular function: transferase activity.
GO:0051539; Molecular function: 4 iron, 4 sulfur cluster binding.
GO:0018339; Biological process: peptidyl-L-beta-methylthioaspartic acid biosynthetic process from peptidyl-aspartic acid.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

PROSITE	PS50926; TRAM; 0-1; trigger=PRU00208;
	PS01278; MTTASE_RADICAL; 1;
	PS51449; MTTASE_N; 1; trigger=PRU00780;
	PS51918; RADICAL_SAM; 1; trigger=PRU01266;
Pfam	PF04055; Radical_SAM; 1;
	PF01938; TRAM; 0-1;
	PF00919; UPF0004; 1;
NCBIfam	TIGR01125; TIGR01125; 1;
	TIGR00089; TIGR00089; 1;

Features [?]

From: RIMO_ECOLI (P0AEI4)

Key From To Description Tag Condition FTGroup

BINDING 17 17 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 53 53 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 82 82 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1 C 1

BINDING 150 150 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C 2

BINDING 154 154 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C 2

BINDING 157 157 /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-S-AdoMet C 2

Additional information [?]

Size range	401-545 amino acids
Related rules	None
Fusion	None

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