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HAMAP rule MF_01885

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General rule information [?]

Accession MF_01885
Dates 21-OCT-2010 (Created)
1-JUN-2023 (Last updated, Version 12)
Name tRNA_methyltr_TrmL
Scope(s) Bacteria
Template(s) P0AGJ7 (TRML_ECOLI); P44868 (TRML_HAEIN); Q74Y93 (TRML_YERPE); [ Recover all ]
Triggered by HAMAP; MF_01885 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier TRML
case <OC:Pseudomonadota>
Protein name RecName: Full=tRNA (cytidine(34)-2'-O)-methyltransferase;
AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL;
Gene name Name=trmL;
Protein name RecName: Full=Putative tRNA (cytidine(34)-2'-O)-methyltransferase;
AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase;
end case

Comments [?]

case <OC:Pseudomonadota>
FUNCTIONMethylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.
FUNCTIONCould methylate the ribose at the nucleotide 34 wobble position in tRNA.
end case
CATALYTIC ACTIVITY Reaction=cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O- methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43084, Rhea:RHEA-COMP:10331, Rhea:RHEA-COMP:10332, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74495, ChEBI:CHEBI:82748; EC=;
CATALYTIC ACTIVITY Reaction=5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S- adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O- methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:43088, Rhea:RHEA-COMP:10333, Rhea:RHEA-COMP:10334, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74508, ChEBI:CHEBI:74511; EC=;
case <OC:Pseudomonadota>
end case
SIMILARITYBelongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.

Keywords [?]

Gene Ontology [?]

GO:0008175; Molecular function:tRNA methyltransferase activity
GO:0008757; Molecular function:S-adenosylmethionine-dependent methyltransferase activity
GO:0030488; Biological process:tRNA methylation
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF00588; SpoU_methylase; 1;
NCBIfam TIGR00185; RRNA_methyl_2; 1;
PIRSF PIRSF029256; SpoU_TrmH_prd; 1;

Features [?]

From: TRML_HAEIN (P44868)
Key From To Description Tag Condition FTGroup
BINDING 78 78 /ligand="S-adenosyl-L-methionine"
BINDING 100 100 /ligand="S-adenosyl-L-methionine"
BINDING 122 122 /ligand="S-adenosyl-L-methionine"
BINDING 130 130 /ligand="S-adenosyl-L-methionine"

Additional information [?]

Size range 140-230 amino acids
Related rules None
Fusion Nter: None Cter: None

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