HAMAP rule MF_01885

General rule information [?]

PURL	https://purl.expasy.org/hamap/rule/MF_01885
Accession	MF_01885
Dates	21-OCT-2010 (Created) 13-MAY-2026 (Last updated, Version )
Name	tRNA_methyltr_TrmL
Scope(s)	Bacteria
Template(s)	P0AGJ7 (TRML_ECOLI); P44868 (TRML_HAEIN); Q74Y93 (TRML_YERPE); O31590 (CSPR_BACSU); [ Recover all ]
Triggered by	HAMAP; MF_01885 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Pseudomonadota>
Identifier	TRML
Protein name	RecName: Full=tRNA (cytidine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase; EC=2.1.1.207; AltName: Full=tRNA (cytidine(34)-2'-O)-methyltransferase; AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase;
Gene name	Name=trmL;
else case <OC:Bacilli>
Identifier	CSPR
Protein name	RecName: Full=tRNA (cytidine(34)/5-carboxymethylaminomethyluridine(34)/guanosine(34)-2'-O)-methyltransferase; EC=2.1.1.207; AltName: Full=tRNA (cytidine(34)-2'-O)-methyltransferase; AltName: Full=tRNA (cytidine/uridine/guanosine-2'-O-)-methyltransferase;
else
Identifier	TRML
Protein name	RecName: Full=Probable tRNA (cytidine(34)/5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase; EC=2.1.1.207; AltName: Full=tRNA (cytidine(34)-2'-O)-methyltransferase; AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase;
end case

Comments [?]

case <OC:Pseudomonadota>
FUNCTION	S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CAA) and tRNA(Leu)(UAA). It leads to the formation of 2'-O-methylcytidine (Cm34) in tRNA(Leu)(CAA) and 5-carboxymethylaminomethyl-2'-O-methyluridine (cmnm5Um34) in tRNA(Leu)(UAA). The enzyme requires the presence of 2-methylthio-N6- isopentenyladenosine at position 37 (ms2i6A37) for activity.
else case <OC:Bacilli>
FUNCTION	S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the ribose at the nucleotide 34 wobble position in tRNA(Leu)(CAA), tRNA(Leu)(UAA) and tRNA(Phe)(GAA). It leads to the formation of 2'-O-methylcytidine (Cm34) in tRNA(Leu)(CAA), 5- carboxymethylaminomethyl-2'-O-methyluridine (cmnm5Um34) in tRNA(Leu)(UAA) and 2'-O-methylguanosine (Gm34) in tRNA(Phe)(GAA). The enzyme requires the presence of 2-methylthio-N6-isopentenyladenosine at position 37 (ms2i6A37) for activity.
else
FUNCTION	S-adenosyl-L-methionine-dependent methyltransferase that probably methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CAA) and tRNA(Leu)(UAA). It leads to the formation of 2'-O-methylcytidine (Cm34) in tRNA(Leu)(CAA) and 5-carboxymethylaminomethyl-2'-O-methyluridine (cmnm5Um34) in tRNA(Leu)(UAA).
end case
CATALYTIC ACTIVITY	Reaction=cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O- methylcytidine(34) in tRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:43084, Rhea:RHEA-COMP:10331, Rhea:RHEA-COMP:10332, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74495, ChEBI:CHEBI:82748; EC=2.1.1.207; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43085;
CATALYTIC ACTIVITY	Reaction=5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S- adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O- methyluridine(34) in tRNA(Leu) + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:43088, Rhea:RHEA-COMP:10333, Rhea:RHEA-COMP:10334, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74508, ChEBI:CHEBI:74511; EC=2.1.1.207; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:43089;
case <OC:Bacilli>
CATALYTIC ACTIVITY	Reaction=guanosine(34) in tRNA + S-adenosyl-L-methionine = 2'-O- methylguanosine(34) in tRNA + S-adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:85171, Rhea:RHEA-COMP:10341, Rhea:RHEA-COMP:20520, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:74269, ChEBI:CHEBI:74445; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:85172;
end case
SUBUNIT	Homodimer.
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.

Keywords [?]

Cytoplasm

Methyltransferase

S-adenosyl-L-methionine

Transferase

tRNA processing

Gene Ontology [?]

GO:0008175; Molecular function:tRNA methyltransferase activity

GO:0008757; Molecular function:S-adenosylmethionine-dependent methyltransferase activity

GO:0030488; Biological process:tRNA methylation

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam	PF00588; SpoU_methylase; 1;
NCBIfam	TIGR00185; RRNA_methyl_2; 1;
PIRSF	PIRSF029256; SpoU_TrmH_prd; 1;

Features [?]

From: TRML_HAEIN (P44868)

Key

From

Description

Tag

Condition

FTGroup

BINDING