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Annotation rule MF_01885
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General rule information [?]

Accession MF_01885
Dates 21-OCT-2010 (Created)
19-NOV-2019 (Last updated, Version 8)
Name tRNA_methyltr_TrmL
Templates P0AGJ7 (TRML_ECOLI); P44868 (TRML_HAEIN); Q74Y93 (TRML_YERPE): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Proteobacteria>
Protein name
RecName: Full=tRNA (cytidine(34)-2'-O)-methyltransferase;
AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL;
Gene name
Protein name
RecName: Full=Putative tRNA (cytidine(34)-2'-O)-methyltransferase;
AltName: Full=tRNA (cytidine/uridine-2'-O-)-methyltransferase;
end case

Comments [?]

case <OC:Proteobacteria>
Function Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.
Function Could methylate the ribose at the nucleotide 34 wobble position in tRNA.
end case
Catalytic activity RHEA:43084: cytidine(34) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(34) in tRNA + H(+) + S-adenosyl-L-homocysteine
RHEA:43088: 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu) + H(+) + S-adenosyl-L-homocysteine
case <OC:Proteobacteria>
Subunit Homodimer.
end case
Subcellular location Cytoplasm.
Similarity Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.

Keywords [?]

Gene Ontology [?]

GO:0008175; Molecular function: tRNA methyltransferase activity.
GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.
GO:0030488; Biological process: tRNA methylation.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00588; SpoU_methylase; 1;
TIGRFAMs TIGR00185; RRNA_methyl_2; 1;
PIRSF PIRSF029256; SpoU_TrmH_prd; 1;

Features [?]

From: TRML_HAEIN (P44868)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING (Optional)     78     78       S-adenosyl-L-methionine; via carbonyl oxygen     [ILVM]  
BINDING (Optional)     100     100       S-adenosyl-L-methionine; via amide nitrogen     G  
BINDING (Optional)     122     122       S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen     [ILVM]  
BINDING (Optional)     130     130       S-adenosyl-L-methionine     S  

Additional information [?]

Size range 140-230 amino acids
Related rules None
Fusion None