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HAMAP rule MF_01897

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General rule information [?]

Accession MF_01897
Dates 3-MAY-2011 (Created)
10-OCT-2023 (Last updated, Version 13)
Name GyrA
Scope(s) Bacteria
Template(s) P0AES4 (GYRA_ECOLI); P9WG47 (GYRA_MYCTU); P37411 (GYRA_SALTY); O67108 (TOP4A_AQUAE); [ Recover all ]
Triggered by HAMAP; MF_01897 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier GYRA
Protein name RecName: Full=DNA gyrase subunit A;
Gene name Name=gyrA;

Comments [?]

FUNCTIONA type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.
CATALYTIC ACTIVITY Reaction=ATP-dependent breakage, passage and rejoining of double- stranded DNA.; EC=;
SUBUNITHeterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.
MISCELLANEOUSFew gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.
SIMILARITYBelongs to the type II topoisomerase GyrA/ParC subunit family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0003677; Molecular function:DNA binding
GO:0003918; Molecular function:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0006265; Biological process:DNA topological change
GO:0006261; Biological process:DNA-templated DNA replication
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF03989; DNA_gyraseA_C; 6;
Pfam PF00521; DNA_topoisoIV; 1;
NCBIfam TIGR01063; GyrA; 1;

Features [?]

Key From To Description Tag Condition FTGroup
ACT_SITE 122 122 /note="O-(5'-phospho-DNA)-tyrosine intermediate" Y
MOTIF 560 566 /note="GyrA-box" motif Q-[KNRHGA]-[RIT]-[GA]-[GT]-[KRVIQH]-G

Additional information [?]

Size range 697-1016 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments The degree of DNA negative supercoiling has been studied in very few bacteria, E.coli gyrase is extra efficient compared to other bacteria. The enzyme from Aquifex aeolicus is a topoisomerase IV; the A subunit has lost the GyrA-box and despite its similarity to GyrA does not have the ability to negatively supercoil DNA. Some members of this family contain inteins.

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