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HAMAP rule MF_01903

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General rule information [?]

Accession MF_01903
Dates 6-DEC-2005 (Created)
7-MAY-2024 (Last updated, Version 33)
Name XGPRT
Scope(s) Bacteria
Pseudomonadota
Template(s) P0A9M5 (XGPT_ECOLI); Q8ZC05 (XGPT_YERPE); [ Recover all ]
Triggered by HAMAP; MF_01903 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier XGPT
Protein name RecName: Full=Xanthine-guanine phosphoribosyltransferase;
                 Short=XGPRT;
                 EC=2.4.2.-;
                 EC=2.4.2.22;
AltName: Full=Xanthine phosphoribosyltransferase;
Gene name Name=gpt;

Comments [?]

FUNCTIONPurine salvage pathway enzyme that catalyzes the transfer of the ribosyl-5-phosphate group from 5-phospho-alpha-D-ribose 1- diphosphate (PRPP) to the N9 position of the 6-oxopurines guanine and xanthine to form the corresponding ribonucleotides GMP (guanosine 5'- monophosphate) and XMP (xanthosine 5'-monophosphate), with the release of PPi. To a lesser extent, also acts on hypoxanthine.
CATALYTIC ACTIVITY Reaction=diphosphate + GMP = 5-phospho-alpha-D-ribose 1-diphosphate + guanine; Xref=Rhea:RHEA:25424, ChEBI:CHEBI:16235, ChEBI:CHEBI:33019, ChEBI:CHEBI:58017, ChEBI:CHEBI:58115; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:25426;
CATALYTIC ACTIVITY Reaction=diphosphate + XMP = 5-phospho-alpha-D-ribose 1-diphosphate + xanthine; Xref=Rhea:RHEA:10800, ChEBI:CHEBI:17712, ChEBI:CHEBI:33019, ChEBI:CHEBI:57464, ChEBI:CHEBI:58017; EC=2.4.2.22; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:10802;
CATALYTIC ACTIVITY Reaction=diphosphate + IMP = 5-phospho-alpha-D-ribose 1-diphosphate + hypoxanthine; Xref=Rhea:RHEA:17973, ChEBI:CHEBI:17368, ChEBI:CHEBI:33019, ChEBI:CHEBI:58017, ChEBI:CHEBI:58053; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:17975;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYPurine metabolism; GMP biosynthesis via salvage pathway; GMP from guanine: step 1/1.
PATHWAYPurine metabolism; XMP biosynthesis via salvage pathway; XMP from xanthine: step 1/1.
SUBUNITHomotetramer.
case not defined <Property:Membrane> or <Property:Membrane=1>
SUBCELLULAR LOCATIONCell membrane; Peripheral membrane protein.
else case <Property:Membrane=2>
SUBCELLULAR LOCATIONCell inner membrane; Peripheral membrane protein.
end case
SIMILARITYBelongs to the purine/pyrimidine phosphoribosyltransferase family. XGPT subfamily.

Keywords [?]

Cell membrane
case defined <Property:Membrane> and <Property:Membrane=2>
Cell inner membrane
end case
Glycosyltransferase
Magnesium
Metal-binding
Membrane
Purine salvage
Transferase

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0000310; Molecular function:xanthine phosphoribosyltransferase activity
GO:0032263; Biological process:GMP salvage
GO:0032265; Biological process:XMP salvage
GO:0005886; Cellular component:plasma membrane

Cross-references [?]

PROSITE PS00103; PUR_PYR_PR_TRANSFER; 1;
Pfam PF00156; Pribosyltran; 1;

Features [?]

From: XGPT_ECOLI (P0A9M5)
Key From To Description Tag Condition FTGroup
BINDING 92 96 /ligand="GMP"
/ligand_id="ChEBI:CHEBI:58115"		 D-[TS]-G-x-T
BINDING 134 135 /ligand="GMP"
/ligand_id="ChEBI:CHEBI:58115"		 W-[IV]
BINDING 37 38 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"		 R-G
BINDING 88 96 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"
BINDING 89 89 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D
BINDING 69 69 /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
/ligand_id="ChEBI:CHEBI:58017"		 [RKQ]
BINDING 69 69 /ligand="GMP"
/ligand_id="ChEBI:CHEBI:58115"		 [RKQ]
BINDING 92 92 /ligand="guanine"
/ligand_id="ChEBI:CHEBI:16235"		 D
BINDING 92 92 /ligand="xanthine"
/ligand_id="ChEBI:CHEBI:17712"		 D
BINDING 135 135 /ligand="guanine"
/ligand_id="ChEBI:CHEBI:16235"		 [IV]
BINDING 135 135 /ligand="xanthine"
/ligand_id="ChEBI:CHEBI:17712"		 [IV]

Additional information [?]

Size range 152-176 amino acids
Related rules None
Fusion Nter: None Cter: None



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