HAMAP rule MF_01926

General rule information [?]

Accession	MF_01926
Dates	2-JUL-2014 (Created) 1-JUN-2023 (Last updated, Version 6)
Name	PurS
Scope(s)	Bacteria Archaea
Template(s)	P12049 (PURS_BACSU); Q9X0X1 (PURS_THEMA); [ Recover all ]
Triggered by	HAMAP; MF_01926 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	PURS
Protein name	RecName: Full=Phosphoribosylformylglycinamidine synthase subunit PurS; Short=FGAM synthase; EC=6.3.5.3; AltName: Full=Formylglycinamide ribonucleotide amidotransferase subunit III; Short=FGAR amidotransferase III; Short=FGAR-AT III; AltName: Full=Phosphoribosylformylglycinamidine synthase subunit III;
Gene name	Name=purS;

Comments [?]

FUNCTION	Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP- dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.
CATALYTIC ACTIVITY	Reaction=ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D- ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D- ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:17129, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:147286, ChEBI:CHEBI:147287, ChEBI:CHEBI:456216; EC=6.3.5.3;
PATHWAY	Purine metabolism; IMP biosynthesis via de novo pathway; 5- amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5- phospho-D-ribosyl)glycinamide: step 1/2.
SUBUNIT	Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
SUBCELLULAR LOCATION	Cytoplasm.
SIMILARITY	Belongs to the PurS family.

Keywords [?]

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding

GO:0004642; Molecular function:phosphoribosylformylglycinamidine synthase activity

GO:0006189; Biological process:'de novo' IMP biosynthetic process

GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam	PF02700; PurS; 1;
NCBIfam	TIGR00302; TIGR00302; 1;

Features [?]

Additional information [?]

Size range	70-130 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).