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HAMAP rule MF_01926

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General rule information [?]

Accession MF_01926
Dates 2-JUL-2014 (Created)
1-JUN-2023 (Last updated, Version 6)
Name PurS
Scope(s) Bacteria
Archaea
Template(s) P12049 (PURS_BACSU); Q9X0X1 (PURS_THEMA); [ Recover all ]
Triggered by HAMAP; MF_01926 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PURS
Protein name RecName: Full=Phosphoribosylformylglycinamidine synthase subunit PurS;
                 Short=FGAM synthase;
                 EC=6.3.5.3;
AltName: Full=Formylglycinamide ribonucleotide amidotransferase subunit III;
                 Short=FGAR amidotransferase III;
                 Short=FGAR-AT III;
AltName: Full=Phosphoribosylformylglycinamidine synthase subunit III;
Gene name Name=purS;

Comments [?]

FUNCTIONPart of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP- dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.
CATALYTIC ACTIVITY Reaction=ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D- ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D- ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate; Xref=Rhea:RHEA:17129, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:147286, ChEBI:CHEBI:147287, ChEBI:CHEBI:456216; EC=6.3.5.3;
PATHWAYPurine metabolism; IMP biosynthesis via de novo pathway; 5- amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5- phospho-D-ribosyl)glycinamide: step 1/2.
SUBUNITPart of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the PurS family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0004642; Molecular function:phosphoribosylformylglycinamidine synthase activity
GO:0006189; Biological process:'de novo' IMP biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF02700; PurS; 1;
NCBIfam TIGR00302; TIGR00302; 1;

Features [?]


Additional information [?]

Size range 70-130 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).



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