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Annotation rule MF_01926
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General rule information [?]

Accession MF_01926
Dates 2-JUL-2014 (Created)
23-MAR-2020 (Last updated, Version 5)
Name PurS
Scope
Bacteria
Archaea
Templates P12049 (PURS_BACSU); Q9X0X1 (PURS_THEMA): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PURS
Protein name
RecName: Full=Phosphoribosylformylglycinamidine synthase subunit PurS;
Short=FGAM synthase;
EC 6.3.5.3;
AltName: Full=Formylglycinamide ribonucleotide amidotransferase subunit III;
Short=FGAR amidotransferase III;
Short=FGAR-AT III;
AltName: Full=Phosphoribosylformylglycinamidine synthase subunit III;
Gene name
purS

Comments [?]

Function Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.
Catalytic activity RHEA:17129: ATP + H2O + L-glutamine + N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ADP + H(+) + L-glutamate + phosphate
EC 6.3.5.3
Pathway Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subunit Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.
Subcellular location Cytoplasm.
Similarity Belongs to the PurS family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0004642; Molecular function: phosphoribosylformylglycinamidine synthase activity.
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF02700; PurS; 1;
TIGRFAMs TIGR00302; TIGR00302; 1;

Additional information [?]

Size range 70-130 amino acids
Related rules None
Fusion None
Comments FGAM synthase can be composed of a single polypeptide (large PurL, MF_00419), or it can be composed of three separate proteins: PurL (small PurL, MF_00420), PurQ (MF_00421) and PurS (MF_01926).