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Annotation rule MF_01931
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General rule information [?]

Accession MF_01931
Dates 4-SEP-2014 (Created)
19-NOV-2019 (Last updated, Version 5)
Name PurF
Scope
Bacteria
Archaea
Templates P0AG16 (PUR1_ECOLI); P00497 (PUR1_BACSU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PUR1
Protein name
RecName: Full=Amidophosphoribosyltransferase;
Short=ATase;
EC 2.4.2.14;
AltName: Full=Glutamine phosphoribosylpyrophosphate amidotransferase;
Short=GPATase;
Gene name
purF

Comments [?]

Function Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.
Catalytic activity RHEA:14905: 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5-phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine
EC 2.4.2.14
case <FTGroup:1>
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) ion per subunit.
end case
case <FTGroup:2>
Cofactor [4Fe-4S] cluster
Note: Binds 1 [4Fe-4S] cluster per subunit.
end case
Pathway Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Similarity In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords [?]

case <FTGroup:1>
end case
case <FTGroup:2>
end case

Gene Ontology [?]

GO:0004044; Molecular function: amidophosphoribosyltransferase activity.
case <FTGroup:1>
GO:0000287; Molecular function: magnesium ion binding.
end case
case <FTGroup:2>
GO:0051536; Molecular function: iron-sulfur cluster binding.
end case
GO:0006189; Biological process: 'de novo' IMP biosynthetic process.

Cross-references [?]

PROSITE PS51278; GATASE_TYPE_2; 1; trigger=PRU00609;
PS00103; PUR_PYR_PR_TRANSFER; 1;
Pfam PF00310; GATase_2; 0-1;
PF00156; Pribosyltran; 1;
PF13537; GATase_7; 0-1;
TIGRFAMs TIGR01134; purF; 1;
PIRSF PIRSF000485; Amd_phspho_trans; 1;

Features [?]

From: PUR1_BACSU (P00497)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     12     12       Nucleophile     C  
METAL (Optional)     247     247       Iron-sulfur (4Fe-4S)     C   2
METAL     294     294       Magnesium     [ST]   1
METAL     356     356       Magnesium     D   1
METAL     357     357       Magnesium     D   1
METAL (Optional)     393     393       Iron-sulfur (4Fe-4S)     C   2
METAL (Optional)     448     448       Iron-sulfur (4Fe-4S)     C   2
METAL (Optional)     451     451       Iron-sulfur (4Fe-4S)     C   2

Additional information [?]

Size range 430-600 amino acids
Related rules None
Fusion None
Comments The N-terminus is cleaved. The mature enzyme starts with cysteine (active site).