HAMAP rule MF_01931
General rule information
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| Accession | MF_01931 |
| Dates | 4-SEP-2014 (Created)
3-SEP-2024 (Last updated, Version 9) |
| Name | PurF |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P0AG16 (PUR1_ECOLI); P00497 (PUR1_BACSU); [ Recover all ] |
| Triggered by |
HAMAP; MF_01931 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PUR1 |
| Protein name | RecName: Full=Amidophosphoribosyltransferase; Short=ATase; EC=2.4.2.14; AltName: Full=Glutamine phosphoribosylpyrophosphate amidotransferase; Short=GPATase; |
| Gene name | Name=purF; |
Comments
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| FUNCTION | Catalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine. |
| CATALYTIC ACTIVITY | Reaction=5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate = 5- phospho-alpha-D-ribose 1-diphosphate + L-glutamine + H2O; Xref=Rhea:RHEA:14905, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:33019, ChEBI:CHEBI:58017, ChEBI:CHEBI:58359, ChEBI:CHEBI:58681; EC=2.4.2.14; |
| case <FTGroup:1> | |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
| end case | |
| case <FTGroup:2> | |
| COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit.; |
| end case | |
| PATHWAY | Purine metabolism; IMP biosynthesis via de novo pathway; N(1)- (5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1- diphosphate: step 1/2. |
| SIMILARITY | In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. |
Keywords
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| Glutamine amidotransferase | |
| Glycosyltransferase | |
| Purine biosynthesis | |
| Transferase | |
| case <FTGroup:1> | |
| Magnesium | |
| Metal-binding | |
| end case | |
| case <FTGroup:2> | |
| 4Fe-4S | |
| Iron | |
| Iron-sulfur | |
| Metal-binding | |
| end case | |
Gene Ontology
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| GO:0004044; Molecular function:amidophosphoribosyltransferase activity | |
| case <FTGroup:1> | |
| GO:0000287; Molecular function:magnesium ion binding | |
| end case | |
| case <FTGroup:2> | |
| GO:0051536; Molecular function:iron-sulfur cluster binding | |
| end case | |
| GO:0006189; Biological process:'de novo' IMP biosynthetic process | |
Cross-references
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| PROSITE | PS51278; GATASE_TYPE_2; 1; |
| PROSITE | PS00103; PUR_PYR_PR_TRANSFER; 1; |
| Pfam | PF00310; GATase_2; 0-1; |
| Pfam | PF00156; Pribosyltran; 1; |
| Pfam | PF13537; GATase_7; 0-1; |
| NCBIfam | TIGR01134; purF; 1; |
| PIRSF | PIRSF000485; Amd_phspho_trans; 1; |
Features
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| From: PUR1_BACSU (P00497) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 12 | 12 | /note="Nucleophile" | C | ||||||||
| BINDING | 247 | 247 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 2 | |||||||
| BINDING | 294 | 294 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
[ST] | 1 | |||||||
| BINDING | 356 | 356 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 357 | 357 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | 1 | |||||||
| BINDING | 393 | 393 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 2 | |||||||
| BINDING | 448 | 448 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 2 | |||||||
| BINDING | 451 | 451 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" |
C | 2 | |||||||
Additional information
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| Size range | 430-600 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | The N-terminus is cleaved. The mature enzyme starts with cysteine (active site). |