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HAMAP rule MF_01931

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General rule information [?]

Accession MF_01931
Dates 4-SEP-2014 (Created)
1-JUN-2023 (Last updated, Version 8)
Name PurF
Scope(s) Bacteria
Archaea
Template(s) P0AG16 (PUR1_ECOLI); P00497 (PUR1_BACSU); [ Recover all ]
Triggered by HAMAP; MF_01931 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PUR1
Protein name RecName: Full=Amidophosphoribosyltransferase;
                 Short=ATase;
                 EC=2.4.2.14;
AltName: Full=Glutamine phosphoribosylpyrophosphate amidotransferase;
                 Short=GPATase;
Gene name Name=purF;

Comments [?]

FUNCTIONCatalyzes the formation of phosphoribosylamine from phosphoribosylpyrophosphate (PRPP) and glutamine.
CATALYTIC ACTIVITY Reaction=5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = 5- phospho-alpha-D-ribose 1-diphosphate + H2O + L-glutamine; Xref=Rhea:RHEA:14905, ChEBI:CHEBI:15377, ChEBI:CHEBI:29985, ChEBI:CHEBI:33019, ChEBI:CHEBI:58017, ChEBI:CHEBI:58359, ChEBI:CHEBI:58681; EC=2.4.2.14;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
case <FTGroup:2>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster per subunit.;
end case
PATHWAYPurine metabolism; IMP biosynthesis via de novo pathway; N(1)- (5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1- diphosphate: step 1/2.
SIMILARITYIn the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords [?]


Gene Ontology [?]

GO:0004044; Molecular function:amidophosphoribosyltransferase activity
case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
case <FTGroup:2>
GO:0051536; Molecular function:iron-sulfur cluster binding
end case
GO:0006189; Biological process:'de novo' IMP biosynthetic process

Cross-references [?]

PROSITE PS51278; GATASE_TYPE_2; 1;
PROSITE PS00103; PUR_PYR_PR_TRANSFER; 1;
Pfam PF00310; GATase_2; 0-1;
Pfam PF00156; Pribosyltran; 1;
Pfam PF13537; GATase_7; 0-1;
NCBIfam TIGR01134; purF; 1;
PIRSF PIRSF000485; Amd_phspho_trans; 1;

Features [?]

From: PUR1_BACSU (P00497)
Key From To Description Tag Condition FTGroup
ACT_SITE 12 12 /note="Nucleophile" C
BINDING 247 247 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
C 2
BINDING 294 294 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
[ST] 1
BINDING 356 356 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D 1
BINDING 357 357 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D 1
BINDING 393 393 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
C 2
BINDING 448 448 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
C 2
BINDING 451 451 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
C 2

Additional information [?]

Size range 430-600 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments The N-terminus is cleaved. The mature enzyme starts with cysteine (active site).



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