HAMAP rule MF_01939
General rule information
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| Accession | MF_01939 |
| Dates | 24-NOV-2014 (Created)
1-JUN-2023 (Last updated, Version 9) |
| Name | PrpB |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P77541 (PRPB_ECOLI); Q56062 (PRPB_SALTY); Q937P0 (PRPB_CUPNE); [ Recover all ] |
| Triggered by |
HAMAP; MF_01939 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | PRPB |
| Protein name | RecName: Full=2-methylisocitrate lyase; Short=MICL; Short=2-MIC; EC=4.1.3.30; AltName: Full=(2R,3S)-2-methylisocitrate lyase; |
| Gene name | Name=prpB; |
Comments
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| FUNCTION | Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2- methylisocitrate to yield pyruvate and succinate via an alpha-carboxy- carbanion intermediate. |
| CATALYTIC ACTIVITY | Reaction=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate; Xref=Rhea:RHEA:16809, ChEBI:CHEBI:15361, ChEBI:CHEBI:30031, ChEBI:CHEBI:57429; EC=4.1.3.30; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| PATHWAY | Organic acid metabolism; propanoate degradation. |
| SUBUNIT | Homotetramer; dimer of dimers. |
| SIMILARITY | Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family. |
Keywords
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Gene Ontology
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| GO:0000287; Molecular function:magnesium ion binding |
| GO:0046421; Molecular function:methylisocitrate lyase activity |
| GO:0019629; Biological process:propionate catabolic process, 2-methylcitrate cycle |
Cross-references
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Features
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| From: PRPB_ECOLI (P77541) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 45 | 47 | /ligand="substrate" | S-x-[GA] | ||||||||
| BINDING | 123 | 124 | /ligand="substrate" | C-G | ||||||||
| BINDING | 210 | 212 | /ligand="substrate" | N-x-T | ||||||||
| BINDING | 85 | 85 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 87 | 87 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
| BINDING | 158 | 158 | /ligand="substrate" | R | ||||||||
| BINDING | 188 | 188 | /ligand="substrate" | E | ||||||||
| BINDING | 241 | 241 | /ligand="substrate" | R | ||||||||
| BINDING | 270 | 270 | /ligand="substrate" | R | ||||||||
Additional information
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| Size range | 267-332 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |