![]() |
|
Annotation rule MF_01939 |
Accession | MF_01939 |
Dates | 24-NOV-2014 (Created) 19-NOV-2019 (Last updated, Version 5) |
Name | PrpB |
Scope | Bacteria
Archaea |
Templates | P77541 (PRPB_ECOLI); Q56062 (PRPB_SALTY); Q937P0 (PRPB_CUPNE): [Recover all] |
Triggered by |
Identifier |
|
Protein name |
|
Gene name |
|
Function | Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate via an alpha-carboxy-carbanion intermediate. |
Catalytic activity | RHEA:16809: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
EC 4.1.3.30 |
Cofactor | Mg(2+) |
Pathway | Organic acid metabolism; propanoate degradation. |
Subunit | Homotetramer; dimer of dimers. |
Similarity | Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family. |
From: PRPB_ECOLI (P77541) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 45 | 47 | Substrate binding | S-x-[GA] | ||||||||
REGION | 123 | 124 | Substrate binding | C-G | ||||||||
REGION | 210 | 212 | Substrate binding | N-x-T | ||||||||
METAL | 85 | 85 | Magnesium | D | ||||||||
METAL | 87 | 87 | Magnesium | D | ||||||||
BINDING | 158 | 158 | Substrate | R | ||||||||
BINDING | 188 | 188 | Substrate | E | ||||||||
BINDING | 241 | 241 | Substrate | R | ||||||||
BINDING | 270 | 270 | Substrate | R |
Size range | 267-332 amino acids |
Related rules | None |
Fusion | None |