HAMAP logo

HAMAP rule MF_01939

Send feedback

General rule information [?]

Accession MF_01939
Dates 24-NOV-2014 (Created)
1-JUN-2023 (Last updated, Version 9)
Name PrpB
Scope(s) Bacteria
Template(s) P77541 (PRPB_ECOLI); Q56062 (PRPB_SALTY); Q937P0 (PRPB_CUPNE); [ Recover all ]
Triggered by HAMAP; MF_01939 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PRPB
Protein name RecName: Full=2-methylisocitrate lyase;
AltName: Full=(2R,3S)-2-methylisocitrate lyase;
Gene name Name=prpB;

Comments [?]

FUNCTIONInvolved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2- methylisocitrate to yield pyruvate and succinate via an alpha-carboxy- carbanion intermediate.
CATALYTIC ACTIVITY Reaction=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate; Xref=Rhea:RHEA:16809, ChEBI:CHEBI:15361, ChEBI:CHEBI:30031, ChEBI:CHEBI:57429; EC=;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
PATHWAYOrganic acid metabolism; propanoate degradation.
SUBUNITHomotetramer; dimer of dimers.
SIMILARITYBelongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.

Keywords [?]

Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0046421; Molecular function:methylisocitrate lyase activity
GO:0019629; Biological process:propionate catabolic process, 2-methylcitrate cycle

Cross-references [?]

NCBIfam TIGR02317; prpB; 1;

Features [?]

From: PRPB_ECOLI (P77541)
Key From To Description Tag Condition FTGroup
BINDING 45 47 /ligand="substrate" S-x-[GA]
BINDING 123 124 /ligand="substrate" C-G
BINDING 210 212 /ligand="substrate" N-x-T
BINDING 85 85 /ligand="Mg(2+)"
BINDING 87 87 /ligand="Mg(2+)"
BINDING 158 158 /ligand="substrate" R
BINDING 188 188 /ligand="substrate" E
BINDING 241 241 /ligand="substrate" R
BINDING 270 270 /ligand="substrate" R

Additional information [?]

Size range 267-332 amino acids
Related rules None
Fusion Nter: None Cter: None

View rule in raw text format (no links)