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Annotation rule MF_01939
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General rule information [?]

Accession MF_01939
Dates 24-NOV-2014 (Created)
14-MAY-2022 (Last updated, Version 7)
Name PrpB
Scope
Bacteria
Archaea
Templates P77541 (PRPB_ECOLI); Q56062 (PRPB_SALTY); Q937P0 (PRPB_CUPNE): [Recover all]
Triggered by

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PRPB
Protein name
RecName: Full=2-methylisocitrate lyase;
Short=MICL;
Short=2-MIC;
EC 4.1.3.30;
AltName: Full=(2R,3S)-2-methylisocitrate lyase;
Gene name
prpB

Comments [?]

Function Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate via an alpha-carboxy-carbanion intermediate.
Catalytic activity RHEA:16809: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
EC 4.1.3.30
Cofactor Mg(2+)
Pathway Organic acid metabolism; propanoate degradation.
Subunit Homotetramer; dimer of dimers.
Similarity Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.

Keywords [?]

Lyase
Magnesium
Metal-binding

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0046421; Molecular function: methylisocitrate lyase activity.
GO:0019629; Biological process: propionate catabolic process, 2-methylcitrate cycle.

Cross-references [?]

PROSITE PS00161; ISOCITRATE_LYASE; 1;
TIGRFAMs TIGR02317; prpB; 1;

Features [?]

From: PRPB_ECOLI (P77541)
Key     From     To       Description   Tag   Condition   FTGroup
BINDING     45     47       /ligand="substrate     S-x-[GA]  
BINDING     123     124       /ligand="substrate     C-G  
BINDING     210     212       /ligand="substrate     N-x-T  
BINDING     85     85       /ligand="Mg(2+)" /ligand_id="18420     D  
BINDING     87     87       /ligand="Mg(2+)" /ligand_id="18420     D  
BINDING     158     158       /ligand="substrate     R  
BINDING     188     188       /ligand="substrate     E  
BINDING     241     241       /ligand="substrate     R  
BINDING     270     270       /ligand="substrate     R  

Additional information [?]

Size range 267-332 amino acids
Related rules None
Fusion None


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