HAMAP rule MF_01939

General rule information [?]

Accession	MF_01939
Dates	24-NOV-2014 (Created) 1-JUN-2023 (Last updated, Version 9)

Name

PrpB

Scope

Bacteria

Archaea

Templates

P77541 (PRPB_ECOLI); Q56062 (PRPB_SALTY); Q937P0 (PRPB_CUPNE): [Recover all]

Triggered by

HAMAP; MF_01939 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PRPB

Protein name

RecName:		Full=2-methylisocitrate lyase;
		Short=MICL;
		Short=2-MIC;
		EC 4.1.3.30;
AltName:		Full=(2R,3S)-2-methylisocitrate lyase;

Gene name

prpB

Comments [?]

Function	Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate via an alpha-carboxy-carbanion intermediate.
Catalytic activity	RHEA:16809: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate EC 4.1.3.30
Cofactor	Mg(2+)
Pathway	Organic acid metabolism; propanoate degradation.
Subunit	Homotetramer; dimer of dimers.
Similarity	Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.

Keywords [?]

Lyase
Magnesium
Metal-binding

Gene Ontology [?]

GO:0000287; Molecular function: magnesium ion binding.
GO:0046421; Molecular function: methylisocitrate lyase activity.
GO:0019629; Biological process: propionate catabolic process, 2-methylcitrate cycle.

Cross-references [?]

PROSITE	PS00161; ISOCITRATE_LYASE; 1;
NCBIfam	TIGR02317; prpB; 1;

Features [?]

From: PRPB_ECOLI (P77541)

Key From To Description Tag Condition FTGroup

BINDING 45 47 /ligand="substrate S-x-[GA]

BINDING 123 124 /ligand="substrate C-G

BINDING 210 212 /ligand="substrate N-x-T

BINDING 85 85 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 87 87 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420 D

BINDING 158 158 /ligand="substrate R

BINDING 188 188 /ligand="substrate E

BINDING 241 241 /ligand="substrate R

BINDING 270 270 /ligand="substrate R

Additional information [?]

Size range	267-332 amino acids
Related rules	None
Fusion	None

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