HAMAP rule MF_01974

General rule information [?]

Accession	MF_01974
Dates	31-MAY-2013 (Created) 1-JUN-2023 (Last updated, Version 11)

Name

MetAP_1

Scope

Bacteria

Templates

P0AE18 (MAP1_ECOLI); P9WK19 (MAP12_MYCTU); Q9ZCD3 (MAP1_RICPR): [Recover all]

case <OC:Bacteria>

Triggered by

HAMAP; MF_01974 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

MAP1

Protein name

RecName:		Full=Methionine aminopeptidase;
		Short=MAP;
		Short=MetAP;
		EC 3.4.11.18;
AltName:		Full=Peptidase M;

Gene name

map

Comments [?]

Function	Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.
Catalytic activity	Reaction=Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.; EC=3.4.11.18;
Cofactor	Co(2+) Zn(2+) Mn(2+) Fe(2+) Note: Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.
Subunit	Monomer.
Similarity	Belongs to the peptidase M24A family. Methionine aminopeptidase type 1 subfamily.

Keywords [?]

Aminopeptidase
Hydrolase
Protease
Metal-binding

Gene Ontology [?]

GO:0046872; Molecular function: metal ion binding.
GO:0070006; Molecular function: metalloaminopeptidase activity.
GO:0004239; Molecular function: initiator methionyl aminopeptidase activity.

Cross-references [?]

PROSITE	PS00680; MAP_1; 1;
Pfam	PF00557; Peptidase_M24; 1;
PRINTS	PR00599; MAPEPTIDASE; 1;
NCBIfam	TIGR00500; met_pdase_I; 1;

Features [?]

From: MAP1_ECOLI (P0AE18)

Key From To Description Tag Condition FTGroup

BINDING 97 97 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1 D 1

BINDING 108 108 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1 D 1

BINDING 108 108 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic D 1

BINDING 171 171 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic H 1

BINDING 204 204 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic E 1

BINDING 235 235 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="1 E 1

BINDING 235 235 /ligand="a divalent metal cation" /ligand_id="ChEBI:CHEBI:60240" /ligand_label="2" /ligand_note="catalytic E 1

BINDING 79 79 /ligand="substrate H

BINDING 178 178 /ligand="substrate H

Additional information [?]

Size range	248-305 amino acids
Related rules	None
Fusion	None

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