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HAMAP rule MF_01977

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General rule information [?]

Accession MF_01977
Dates 16-MAY-2014 (Created)
19-NOV-2022 (Last updated, Version 11)
Name Phosphofructokinase_II_P
Templates P29495 (PFP_PROFC); G4STG9 (PFP_META2); Q3KSV5 (PFP_METMH); Q9NGP6 (PFP_MASBA): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Pyrophosphate--fructose 6-phosphate 1-phosphotransferase;
AltName: Full=6-phosphofructokinase, pyrophosphate dependent;
AltName: Full=PPi-dependent phosphofructokinase;
AltName: Full=Pyrophosphate-dependent 6-phosphofructose-1-kinase;
case <OC:Bacteria>
Gene name
end case

Comments [?]

Function Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions.
Catalytic activity RHEA:13613: beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose 1,6-bisphosphate + H(+) + phosphate
Cofactor Mg(2+)
Activity regulation Non-allosteric.
Pathway Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit Homodimer or homotetramer.
Subcellular location Cytoplasm.
Similarity Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'P' sub-subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003872; Molecular function: 6-phosphofructokinase activity.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00365; PFK; 1;
PIRSF PIRSF036484; PPi-PFK_SMc01852; 1;

Features [?]

From: PFP_PROFC (P29495)
Key     From     To       Description   Tag   Condition   FTGroup
SITE     122     122       Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP     D  
BINDING     149     151       /ligand="substrate     T-x-D  
BINDING     194     196       /ligand="substrate     M-G-R  
BINDING     323     326       /ligand="substrate     [HY]-x(2)-R  
ACT_SITE     151     151       Proton acceptor     D  
BINDING     121     121       /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="catalytic     [DE]  
BINDING     266     266       /ligand="substrate     E  
BINDING     12     12       /ligand="diphosphate" /ligand_id="ChEBI:CHEBI:33019     G  
SITE     148     148       Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi     K  

Additional information [?]

Size range 400-436 amino acids
Related rules None
Fusion None
Comments Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511).