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Annotation rule MF_01978 |
Accession | MF_01978 |
Dates | 16-MAY-2014 (Created) 19-NOV-2019 (Last updated, Version 8) |
Name | Phosphofructokinase_II_B2 |
Scope | Bacteria |
Templates | Q609I3 (PFP_METCA); Q2RNU4 (PFP_RHORT); B0RP51 (PFP_XANCB): [Recover all] |
Triggered by |
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Protein name |
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Gene name |
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Function | Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis. Consistently, PPi-PFK can replace the enzymes of both the forward (ATP-PFK) and reverse (fructose-bisphosphatase (FBPase)) reactions. |
Catalytic activity | RHEA:13613: beta-D-fructose 6-phosphate + diphosphate = beta-D-fructose 1,6-bisphosphate + H(+) + phosphate
EC 2.7.1.90 |
Cofactor | Mg(2+) |
Activity regulation | Non-allosteric. |
Pathway | Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. |
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Clade 'B2' sub-subfamily. |
Pfam | PF00365; PFK; 1; |
PIRSF | PIRSF036483; PFK_XF0274; 1; |
PRINTS | PR00476; PHFRCTKINASE; 1; |
From: PFP_METCA (Q609I3) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
SITE | 115 | 115 | Important for catalytic activity and substrate specificity; stabilizes the transition state when the phosphoryl donor is PPi; prevents ATP from binding by mimicking the alpha-phosphate group of ATP | D | ||||||||
REGION | 142 | 144 | Substrate binding | T-x-D | ||||||||
REGION | 190 | 192 | Substrate binding | [MLV]-G-R | ||||||||
REGION | 297 | 300 | Substrate binding | Y-x(2)-R | ||||||||
ACT_SITE | 144 | 144 | Proton acceptor | D | ||||||||
METAL | 114 | 114 | Magnesium; catalytic | N | ||||||||
BINDING | 247 | 247 | Substrate | E | ||||||||
BINDING | 13 | 13 | Diphosphate; via amide nitrogen | G | ||||||||
SITE | 141 | 141 | Important for catalytic activity; stabilizes the transition state when the phosphoryl donor is PPi | K |
Size range | 384-432 amino acids |
Related rules | None |
Fusion | None |
Comments | Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511). |