HAMAP rule MF_01981

General rule information [?]

Accession	MF_01981
Dates	16-MAY-2014 (Created) 19-NOV-2022 (Last updated, Version 10)

Name	Phosphofructokinase_II_X

Scope

Bacteria

Template

Q8VU09 (PFKA_AMYME)

case <OC:Bacteria>

Triggered by

HAMAP; MF_01981 (Get profile general information and statistics)

end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier

PFKA

Protein name

RecName:		Full=ATP-dependent 6-phosphofructokinase;
		Short=ATP-PFK;
		Short=Phosphofructokinase;
		EC 2.7.1.11;
AltName:		Full=Phosphohexokinase;

Gene name

pfkA

Comments [?]

Function	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity	RHEA:16109: ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+) EC 2.7.1.11
Cofactor	Mg(2+)
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit	Homodimer.
Subcellular location	Cytoplasm.
Similarity	Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.

Keywords [?]

Cytoplasm
Kinase
Transferase
Glycolysis
ATP-binding
Nucleotide-binding
Magnesium
Metal-binding

Gene Ontology [?]

GO:0003872; Molecular function: 6-phosphofructokinase activity.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam	PF00365; PFK; 1;
PIRSF	PIRSF000534; PPi_PFK_TP0108; 1;
PRINTS	PR00476; PHFRCTKINASE; 1;

Features [?]

From: PFKA_AMYME (Q8VU09)

Key From To Description Tag Condition FTGroup

SITE (Optional) 181 181 Important for substrate specificity; cannot use PPi as phosphoryl donor G

BINDING 154 155 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 [RK]-x

BINDING 179 182 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G-[DE]-G-[STG]

BINDING 89 89 /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 G

BINDING 208 210 /ligand="substrate T-x-D

BINDING 253 255 /ligand="substrate M-G-[RH]

BINDING 368 371 /ligand="substrate [HY]-x(2)-R

ACT_SITE 210 210 Proton acceptor D

BINDING 180 180 /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="catalytic [DE]

BINDING 309 309 /ligand="substrate E

Additional information [?]

Size range	431-461 amino acids
Related rules	MF_03186 (PFKA)
Fusion	None
Comments	Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511).

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