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Annotation rule MF_01981
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General rule information [?]

Accession MF_01981
Dates 16-MAY-2014 (Created)
19-NOV-2019 (Last updated, Version 7)
Name Phosphofructokinase_II_X
Scope
Bacteria
Template Q8VU09 (PFKA_AMYME)
case <OC:Bacteria>
end case


Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
PFKA
Protein name
RecName: Full=ATP-dependent 6-phosphofructokinase;
Short=ATP-PFK;
Short=Phosphofructokinase;
EC 2.7.1.11;
AltName: Full=Phosphohexokinase;
Gene name
pfkA

Comments [?]

Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Catalytic activity RHEA:16109: ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)
EC 2.7.1.11
Cofactor Mg(2+)
Pathway Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily.

Keywords [?]


Gene Ontology [?]

GO:0003872; Molecular function: 6-phosphofructokinase activity.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00365; PFK; 1;
PIRSF PIRSF000534; PPi_PFK_TP0108; 1;
PRINTS PR00476; PHFRCTKINASE; 1;

Features [?]

From: PFKA_AMYME (Q8VU09)
Key     From     To       Description   Tag   Condition   FTGroup
SITE (Optional)     181     181       Important for substrate specificity; cannot use PPi as phosphoryl donor     G  
NP_BIND     154     155       ATP     [RK]-x  
NP_BIND     179     182       ATP     G-[DE]-G-[STG]  
BINDING     89     89       ATP; via amide nitrogen     G  
REGION     208     210       Substrate binding     T-x-D  
REGION     253     255       Substrate binding     M-G-[RH]  
REGION     368     371       Substrate binding     [HY]-x(2)-R  
ACT_SITE     210     210       Proton acceptor     D  
METAL     180     180       Magnesium; catalytic     [DE]  
BINDING     309     309       Substrate     E  

Additional information [?]

Size range 431-461 amino acids
Related rules MF_03186 (PFKA)
Fusion None
Comments Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511).