HAMAP rule MF_01981
General rule information
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Accession | MF_01981 |
Dates | 16-MAY-2014 (Created) 19-NOV-2022 (Last updated, Version 10) |
Name | Phosphofructokinase_II_X |
Scope | Bacteria |
Template | Q8VU09 (PFKA_AMYME) |
case <OC:Bacteria>
Triggered by |
end case
Propagated annotation
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Identifier, protein and gene names
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Identifier |
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Protein name |
|
Gene name |
|
Comments
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Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. |
Catalytic activity | RHEA:16109: ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate + H(+)
EC 2.7.1.11 |
Cofactor | Mg(2+) |
Pathway | Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. |
Subunit | Homodimer. |
Subcellular location | Cytoplasm. |
Similarity | Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade 'X' sub-subfamily. |
Keywords
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Gene Ontology
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GO:0003872; Molecular function: 6-phosphofructokinase activity.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.
GO:0006096; Biological process: glycolytic process.
GO:0005737; Cellular component: cytoplasm.
Cross-references
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Pfam | PF00365; PFK; 1; |
PIRSF | PIRSF000534; PPi_PFK_TP0108; 1; |
PRINTS | PR00476; PHFRCTKINASE; 1; |
Features
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From: PFKA_AMYME (Q8VU09) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
SITE (Optional) | 181 | 181 | Important for substrate specificity; cannot use PPi as phosphoryl donor | G | ||||||||
BINDING | 154 | 155 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | [RK]-x | ||||||||
BINDING | 179 | 182 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G-[DE]-G-[STG] | ||||||||
BINDING | 89 | 89 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616 | G | ||||||||
BINDING | 208 | 210 | /ligand="substrate | T-x-D | ||||||||
BINDING | 253 | 255 | /ligand="substrate | M-G-[RH] | ||||||||
BINDING | 368 | 371 | /ligand="substrate | [HY]-x(2)-R | ||||||||
ACT_SITE | 210 | 210 | Proton acceptor | D | ||||||||
BINDING | 180 | 180 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="catalytic | [DE] | ||||||||
BINDING | 309 | 309 | /ligand="substrate | E |
Additional information
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Size range | 431-461 amino acids |
Related rules | MF_03186 (PFKA) |
Fusion | None |
Comments | Classification of type A phosphofructokinases into subfamilies was done according to Mueller at al.(2001) (PubMed:11673446) and Bapteste et al.(2003) (PubMed:14585511). |