HAMAP rule MF_02002
General rule information
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| Accession | MF_02002 |
| Dates | 5-DEC-2005 (Created)
1-JUN-2023 (Last updated, Version 22) |
| Name | Ile_tRNA_synth_type1 |
| Scope(s) |
Bacteria |
| Template(s) | P41972 (SYI1_STAAU); P00956 (SYI_ECOLI); P18330 (SYI1_PSEFL); [ Recover all ] |
| Triggered by |
HAMAP; MF_02002 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | SYI |
| Protein name | RecName: Full=Isoleucine--tRNA ligase; EC=6.1.1.5; AltName: Full=Isoleucyl-tRNA synthetase; Short=IleRS; |
| Gene name | Name=ileS; |
Comments
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| FUNCTION | Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile). |
| CATALYTIC ACTIVITY | Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L- isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666, Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528, ChEBI:CHEBI:456215; EC=6.1.1.5; |
| case <FTGroup:1> | |
| COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per subunit.; |
| end case | |
| SUBUNIT | Monomer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| DOMAIN | IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val- tRNA(Ile)). |
| SIMILARITY | Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. |
Keywords
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| case <FT:9> | |
| Acetylation | |
| end case | |
| Aminoacyl-tRNA synthetase | |
| ATP-binding | |
| Cytoplasm | |
| Ligase | |
| Nucleotide-binding | |
| Protein biosynthesis | |
| case <FTGroup:1> | |
| Metal-binding | |
| Zinc | |
| end case | |
Gene Ontology
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| GO:0004822; Molecular function:isoleucine-tRNA ligase activity | |
| GO:0005524; Molecular function:ATP binding | |
| case <FTGroup:1> | |
| GO:0008270; Molecular function:zinc ion binding | |
| end case | |
| GO:0006428; Biological process:isoleucyl-tRNA aminoacylation | |
| GO:0005737; Cellular component:cytoplasm | |
Cross-references
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| PROSITE | PS00178; AA_TRNA_LIGASE_I; 1; |
| Pfam | PF00133; tRNA-synt_1; 1; |
| Pfam | PF06827; zf-FPG_IleRS; 1; |
| PRINTS | PR00984; TRNASYNTHILE; 1; |
| NCBIfam | TIGR00392; IleS; 1; |
Features
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| From: SYI1_STAAU (P41972) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| MOTIF | 57 | 67 | /note="'HIGH' region" | |||||||||
| MOTIF | 595 | 599 | /note="'KMSKS' region" | |||||||||
| BINDING | 886 | 886 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 889 | 889 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 906 | 906 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 909 | 909 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | 1 | |||||||
| BINDING | 554 | 554 | /ligand="L-isoleucyl-5'-AMP" /ligand_id="ChEBI:CHEBI:178002" |
E | ||||||||
| BINDING | 598 | 598 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
K | ||||||||
| From: SYI_ECOLI (P00956) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| case <OC:Escherichia> or <OC:Shigella> | ||||||||||||
| MOD_RES | 183 | 183 | /note="N6-acetyllysine" | K | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 861-988 amino acids |
| Related rules |
MF_02003 |
| Fusion | Nter: None Cter: None |
| Comments | Some species have a second copy of IleRS in another subfamily (MF_02003). |